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Literature summary extracted from
- gamma-Aminobutyrate:alpha-ketoglutarate aminotransferase from Pseudomonas sp. F-126: purification, crystallization, and enzymologic properties (1980), Arch. Biochem. Biophys., 200, 156-164.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 2.6.1.19 | - |
Pseudomonas sp. |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 2.6.1.19 | pyridoxal phosphate protects against heat inactivation | Pseudomonas sp. |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.6.1.19 | 3-Methyl-2-benzothiazolone hydrazone hydrochloride | - |
Pseudomonas sp. |  |
| 2.6.1.19 | Aminooxyacetate | - |
Pseudomonas sp. | |
| 2.6.1.19 | carbonyl reagents | - |
Pseudomonas sp. | |
| 2.6.1.19 | Cu2+ | - |
Pseudomonas sp. | |
| 2.6.1.19 | D-cycloserine | - |
Pseudomonas sp. | |
| 2.6.1.19 | D-penicillamine | - |
Pseudomonas sp. | |
| 2.6.1.19 | DL-cysteine | - |
Pseudomonas sp. | |
| 2.6.1.19 | gabaculine | - |
Pseudomonas sp. | |
| 2.6.1.19 | HgCl2 | - |
Pseudomonas sp. | |
| 2.6.1.19 | hydrazine | - |
Pseudomonas sp. | |
| 2.6.1.19 | hydroxylamine | - |
Pseudomonas sp. | |
| 2.6.1.19 | monoiodoacetate | not | Pseudomonas sp. | |
| 2.6.1.19 | additional information | no inhibition by chelating agents, non-substrate L- or D-amino acids, metal ions | Pseudomonas sp. | |
| 2.6.1.19 | p-chloromercuribenzoate | - |
Pseudomonas sp. | |
| 2.6.1.19 | phenylhydrazine | - |
Pseudomonas sp. | |
| 2.6.1.19 | SH-group reagents | 2-mercaptoethanol or DTT reactivates | Pseudomonas sp. |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.6.1.19 | 2.8 | - |
2-oxoglutarate | pH 8.0, 37°C | Pseudomonas sp. | |
| 2.6.1.19 | 3.3 | 4.8 | 4-aminobutanoate | pH 8.0, 37°C | Pseudomonas sp. | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.6.1.19 | soluble | - |
Pseudomonas sp. | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.6.1.19 | 176000 | 178000 | gel filtration, sedimentation equilibrium centrifugation | Pseudomonas sp. |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.6.1.19 | 4-aminobutanoate + 2-oxoglutarate | Pseudomonas sp. | key-reaction of gamma-aminobutyrate(GABA)-shunt or bypass | 4-oxobutanoate + L-glutamate | - |
? | |
| 2.6.1.19 | 4-aminobutanoate + 2-oxoglutarate | Pseudomonas sp. F-126 | key-reaction of gamma-aminobutyrate(GABA)-shunt or bypass | 4-oxobutanoate + L-glutamate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.6.1.19 | Pseudomonas sp. | - |
F-126 | - |
| 2.6.1.19 | Pseudomonas sp. F-126 | - |
F-126 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.6.1.19 | F-126 | Pseudomonas sp. |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 2.6.1.19 | after inhibition by carbonyl reagents or sulfhydryl reagents enzyme may be reactivated by incubation with pyridoxal 5-phosphate or thiol reagents | Pseudomonas sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.6.1.19 | 46.8 | - |
- |
Pseudomonas sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.6.1.19 | 4-aminobutanoate + 2-oxoglutarate | - |
Pseudomonas sp. | 4-oxobutanoate + L-glutamate | - |
r | |
| 2.6.1.19 | 4-aminobutanoate + 2-oxoglutarate | key-reaction of gamma-aminobutyrate(GABA)-shunt or bypass | Pseudomonas sp. | 4-oxobutanoate + L-glutamate | - |
? | |
| 2.6.1.19 | 4-aminobutanoate + 2-oxoglutarate | - |
Pseudomonas sp. F-126 | 4-oxobutanoate + L-glutamate | - |
r | |
| 2.6.1.19 | 4-aminobutanoate + 2-oxoglutarate | key-reaction of gamma-aminobutyrate(GABA)-shunt or bypass | Pseudomonas sp. F-126 | 4-oxobutanoate + L-glutamate | - |
? | |
| 2.6.1.19 | 4-aminobutanoate + glyoxylate | at 16% of the activity of 2-oxoglutarate | Pseudomonas sp. | 4-oxobutanoate + glycine | - |
r | |
| 2.6.1.19 | 4-aminobutanoate + glyoxylate | at 16% of the activity of 2-oxoglutarate | Pseudomonas sp. F-126 | 4-oxobutanoate + glycine | - |
r | |
| 2.6.1.19 | 4-aminobutanoate + pyruvate | at 7% of the activity of 2-oxoglutarate | Pseudomonas sp. | 4-oxobutanoate + alanine | - |
r | |
| 2.6.1.19 | 4-aminobutanoate + pyruvate | at 7% of the activity of 2-oxoglutarate | Pseudomonas sp. F-126 | 4-oxobutanoate + alanine | - |
r | |
| 2.6.1.19 | cadaverine + 2-oxoglutarate | poor amino donor | Pseudomonas sp. | L-glutamate + ? | - |
r | |
| 2.6.1.19 | D-lysine + 2-oxoglutarate | poor amino donor | Pseudomonas sp. | L-glutamate + 5-aminopentanal | - |
r | |
| 2.6.1.19 | DL-ornithine + 2-oxoglutarate | poor amino donor | Pseudomonas sp. | L-glutamate + 4-methyl-2-oxopentanoate | - |
? | |
| 2.6.1.19 | hypotaurine + 2-oxoglutarate | poor amino donor | Pseudomonas sp. | L-glutamate + 2-oxoethanesulfinic acid | - |
r | |
| 2.6.1.19 | additional information | no substrates are taurine, 3-aminopropane sulfonate, glycine | Pseudomonas sp. | ? | - |
? | |
| 2.6.1.19 | additional information | no substrates are taurine, 3-aminopropane sulfonate, glycine | Pseudomonas sp. F-126 | ? | - |
? | |
| 2.6.1.19 | putrescine + 2-oxoglutarate | poor amino donor | Pseudomonas sp. | L-glutamate + 4-aminobutanal | - |
r | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.6.1.19 | 60 | - |
- |
Pseudomonas sp. |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.6.1.19 | 30 | 60 | linear increase, above 65°C rapid decrease | Pseudomonas sp. |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.6.1.19 | additional information | - |
pyridoxal phosphate protects against heat inactivation | Pseudomonas sp. |
| 2.6.1.19 | 37 | - |
15 min stable, pH 6-10 | Pseudomonas sp. |
| 2.6.1.19 | 60 | - |
up to, at least 15 min stable, pH 8 | Pseudomonas sp. |
| 2.6.1.19 | 65 | - |
above, 15 min, inactivation | Pseudomonas sp. |
| 2.6.1.19 | 70 | - |
15 min stable in the presence of pyridoxal phosphate | Pseudomonas sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.6.1.19 | 8.5 | 9 | - |
Pseudomonas sp. |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.6.1.19 | 6.5 | 11 | no activity above or below | Pseudomonas sp. |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.6.1.19 | 6 | 10 | 15 min stable, 37°C | Pseudomonas sp. |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.6.1.19 | pyridoxal 5'-phosphate | - |
Pseudomonas sp. | |
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