BRENDA - Enzyme Database

Solubilization of 4-hydroxybenzoate-polyprenyltransferase from cell membrane of Pseudomonas putida and its properties

Uchida, K.; Koizumi, N.; Kawaji, H.; Kawahara, K.; Aida, K.; Agric. Biol. Chem. 55, 2299-2305 (1991)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.5.1.39
cardiolipin
-
Pseudomonas putida
2.5.1.39
dipalmitoylphosphatidylcholine
-
Pseudomonas putida
2.5.1.39
phosphatidylethanolamine
-
Pseudomonas putida
2.5.1.39
phosphatidylglycerol
-
Pseudomonas putida
2.5.1.39
Phospholipid
strongly activated by phospholipid extracted from the bacterial cell, phosphatidylethanolamine is most effective
Pseudomonas putida
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.5.1.39
2,4-dinitrophenol
16% inhibition at 1 mM
Pseudomonas putida
2.5.1.39
Ag+
complete inhibition at 0.1 mM
Pseudomonas putida
2.5.1.39
Ca2+
46% inhibition at 1 mM
Pseudomonas putida
2.5.1.39
Cd2+
complete inhibition at 0.1 mM
Pseudomonas putida
2.5.1.39
Co2+
28% inhibition at 0.1 mM
Pseudomonas putida
2.5.1.39
Cu2+
complete inhibition at 0.1 mM
Pseudomonas putida
2.5.1.39
Fe2+
68% inhibition at 0.1 mM
Pseudomonas putida
2.5.1.39
Hg2+
complete inhibition at 0.1 mM
Pseudomonas putida
2.5.1.39
Mn2+
68% inhibition at 0.1 mM
Pseudomonas putida
2.5.1.39
N-ethylmaleimide
67% inhibition at 1 mM
Pseudomonas putida
2.5.1.39
p-chloromercuribenzoate
95% inhibition at 0.01 mM
Pseudomonas putida
2.5.1.39
Zn2+
complete inhibition at 0.1 mM
Pseudomonas putida
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.39
0.054
-
4-hydroxybenzoate
pH 7, 37°C
Pseudomonas putida
2.5.1.39
0.14
-
nonaprenyl diphosphate
pH 7, 37°C
Pseudomonas putida
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.5.1.39
membrane
-
Pseudomonas putida
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.5.1.39
Mg2+
required
Pseudomonas putida
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.5.1.39
Pseudomonas putida
-
IAM 1219
-
2.5.1.39
Pseudomonas putida IAM 1219
-
IAM 1219
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.5.1.39
additional information
-
-
Pseudomonas putida
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.39
solanesyl diphosphate + 4-hydroxybenzoate
i.e. nonaprenyl diphosphate
637001
Pseudomonas putida
diphosphate + 3-solanesyl-4-hydroxybenzoate
-
637001
Pseudomonas putida
?
2.5.1.39
solanesyl diphosphate + 4-hydroxybenzoate
i.e. nonaprenyl diphosphate
637001
Pseudomonas putida IAM 1219
diphosphate + 3-solanesyl-4-hydroxybenzoate
-
637001
Pseudomonas putida IAM 1219
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.5.1.39
37
-
assay at
Pseudomonas putida
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.39
7
7.2
-
Pseudomonas putida
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.5.1.39
cardiolipin
-
Pseudomonas putida
2.5.1.39
dipalmitoylphosphatidylcholine
-
Pseudomonas putida
2.5.1.39
phosphatidylethanolamine
-
Pseudomonas putida
2.5.1.39
phosphatidylglycerol
-
Pseudomonas putida
2.5.1.39
Phospholipid
strongly activated by phospholipid extracted from the bacterial cell, phosphatidylethanolamine is most effective
Pseudomonas putida
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.5.1.39
2,4-dinitrophenol
16% inhibition at 1 mM
Pseudomonas putida
2.5.1.39
Ag+
complete inhibition at 0.1 mM
Pseudomonas putida
2.5.1.39
Ca2+
46% inhibition at 1 mM
Pseudomonas putida
2.5.1.39
Cd2+
complete inhibition at 0.1 mM
Pseudomonas putida
2.5.1.39
Co2+
28% inhibition at 0.1 mM
Pseudomonas putida
2.5.1.39
Cu2+
complete inhibition at 0.1 mM
Pseudomonas putida
2.5.1.39
Fe2+
68% inhibition at 0.1 mM
Pseudomonas putida
2.5.1.39
Hg2+
complete inhibition at 0.1 mM
Pseudomonas putida
2.5.1.39
Mn2+
68% inhibition at 0.1 mM
Pseudomonas putida
2.5.1.39
N-ethylmaleimide
67% inhibition at 1 mM
Pseudomonas putida
2.5.1.39
p-chloromercuribenzoate
95% inhibition at 0.01 mM
Pseudomonas putida
2.5.1.39
Zn2+
complete inhibition at 0.1 mM
Pseudomonas putida
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.39
0.054
-
4-hydroxybenzoate
pH 7, 37°C
Pseudomonas putida
2.5.1.39
0.14
-
nonaprenyl diphosphate
pH 7, 37°C
Pseudomonas putida
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.5.1.39
membrane
-
Pseudomonas putida
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.5.1.39
Mg2+
required
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.5.1.39
additional information
-
-
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.39
solanesyl diphosphate + 4-hydroxybenzoate
i.e. nonaprenyl diphosphate
637001
Pseudomonas putida
diphosphate + 3-solanesyl-4-hydroxybenzoate
-
637001
Pseudomonas putida
?
2.5.1.39
solanesyl diphosphate + 4-hydroxybenzoate
i.e. nonaprenyl diphosphate
637001
Pseudomonas putida IAM 1219
diphosphate + 3-solanesyl-4-hydroxybenzoate
-
637001
Pseudomonas putida IAM 1219
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.5.1.39
37
-
assay at
Pseudomonas putida
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.39
7
7.2
-
Pseudomonas putida