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Literature summary extracted from

  • Gupta, A.; Rudney, H.
    4-Hydroxybenzoate polyprenyltransferase from rat liver (1985), Methods Enzymol., 110, 327-334.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.39 Brij W1 inhibition at 0.5% or more, activation at 0.05% Rattus norvegicus
2.5.1.39 Nonidet P-40 inhibition at 0.5% or more, activation at 0.05% Rattus norvegicus
2.5.1.39 sodium deoxycholate inhibition at 0.5% or more, activation at 0.05% Rattus norvegicus
2.5.1.39 Triton X-100 inhibition at 0.5% or more, activation at 0.01% Rattus norvegicus
2.5.1.39 Tween 80 inhibition at 0.5% or more, activation at 0.01% Rattus norvegicus

General Stability

EC Number General Stability Organism
2.5.1.39 isolated mitochondria can be stored frozen for 2-3 weeks prior to preparation of membrane fragments without any loss of activity, preparation of mitochondrial fragments loses activity after 2 cycles of freezing and thawing Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.39 4-Aminobenzoic acid
-
Rattus norvegicus
2.5.1.39 4-Chlorobenzoic acid
-
Rattus norvegicus
2.5.1.39 bacitracin strong inhibitor Rattus norvegicus
2.5.1.39 Brij W1 inhibition at 0.5% or more, activation at 0.05% Rattus norvegicus
2.5.1.39 Ca2+ Mg2+ can overcome the inhibition Rattus norvegicus
2.5.1.39 Diphenylamine
-
Rattus norvegicus
2.5.1.39 iodoacetamide
-
Rattus norvegicus
2.5.1.39 KCN
-
Rattus norvegicus
2.5.1.39 NaF
-
Rattus norvegicus
2.5.1.39 Nonidet P-40 inhibition at 0.5% or more, activation at 0.05% Rattus norvegicus
2.5.1.39 p-chloromercuribenzoate
-
Rattus norvegicus
2.5.1.39 sodium deoxycholate inhibition at 0.5% or more, activation at 0.05% Rattus norvegicus
2.5.1.39 Triton X-100 inhibition at 0.5% or more, activation at 0.01% Rattus norvegicus
2.5.1.39 Tween 80 inhibition at 0.5% or more, activation at 0.01% Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.39 0.000833
-
nonaprenyl diphosphate pH 7.4, 25°C or 37°C Rattus norvegicus
2.5.1.39 0.00114
-
4-hydroxybenzoate pH 7.4, 25°C or 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.39 mitochondrial inner membrane
-
Rattus norvegicus 5743
-
2.5.1.39 mitochondrion
-
Rattus norvegicus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.39 Mg2+ activation Rattus norvegicus
2.5.1.39 Mg2+ maximal activation: 10 mM Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.39 solanesyl diphosphate + 4-hydroxybenzoate Rattus norvegicus key enzyme in ubiquinone synthesis, it catalyzes the step which brings together the precursor of the benzoquinone ring and the polyisoprenoid side chain, in rat liver the major product is 3-nonaprenyl-4-hydroxybenzoate, in human and guinea pig it is 3-decaprenyl-4-hydroxybenzoate diphosphate + 3-solanesyl-4-hydroxybenzoate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.39 Rattus norvegicus
-
male Sprague-Dawley
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.39 heart
-
Rattus norvegicus
-
2.5.1.39 kidney
-
Rattus norvegicus
-
2.5.1.39 liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.39 solanesyl diphosphate + 4-hydroxybenzoate i.e. nonaprenyl diphosphate Rattus norvegicus diphosphate + 3-solanesyl-4-hydroxybenzoate small amounts of decaprenyl-4-hydroxybenzoate and octaprenylhydroxybenzoate can be found, depending on source of enzyme and substrate used to provide polyprenyl side chains ?
2.5.1.39 solanesyl diphosphate + 4-hydroxybenzoate key enzyme in ubiquinone synthesis, it catalyzes the step which brings together the precursor of the benzoquinone ring and the polyisoprenoid side chain, in rat liver the major product is 3-nonaprenyl-4-hydroxybenzoate, in human and guinea pig it is 3-decaprenyl-4-hydroxybenzoate Rattus norvegicus diphosphate + 3-solanesyl-4-hydroxybenzoate
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.39 7.4
-
-
Rattus norvegicus