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Literature summary extracted from

  • Harada, I.; Noguchi, T.; Kido, R.
    Purification and characterization of aromatic-amino-acid-glyoxylate aminotransferase from monkey and rat liver (1978), Hoppe-Seyler's Z. Physiol. Chem., 359, 481-488.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.60 hydroxylamine
-
Macaca fuscata
2.6.1.60 hydroxylamine
-
Rattus norvegicus
2.6.1.60 Isonicotinic hydrazide
-
Macaca fuscata
2.6.1.60 Isonicotinic hydrazide
-
Rattus norvegicus
2.6.1.60 KCN
-
Macaca fuscata
2.6.1.60 KCN
-
Rattus norvegicus
2.6.1.60 Semicarbazide strong Macaca fuscata
2.6.1.60 Semicarbazide strong Rattus norvegicus
2.6.1.63 hydroxylamine
-
Macaca fuscata
2.6.1.63 hydroxylamine
-
Rattus norvegicus
2.6.1.63 Isonicotinic hydrazide
-
Macaca fuscata
2.6.1.63 Isonicotinic hydrazide
-
Rattus norvegicus
2.6.1.63 KCN
-
Macaca fuscata
2.6.1.63 KCN
-
Rattus norvegicus
2.6.1.63 Semicarbazide
-
Macaca fuscata
2.6.1.63 Semicarbazide
-
Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.60 0.4
-
glyoxylate cosubstrate L-kynurenine, pH 8.0, 37°C Macaca fuscata
2.6.1.60 0.5
-
glyoxylate L-kynurenine, pH 8.0, 37°C Rattus norvegicus
2.6.1.60 1.2
-
L-kynurenine glyoxylate, pH 8.0, 37°C Rattus norvegicus
2.6.1.60 1.5
-
L-kynurenine cosubstrate glyoxylate, pH 8.0, 37°C Macaca fuscata
2.6.1.63 0.4
-
glyoxylate
-
Macaca fuscata
2.6.1.63 0.5
-
glyoxylate
-
Rattus norvegicus
2.6.1.63 1.2
-
L-kynurenine
-
Rattus norvegicus
2.6.1.63 1.5
-
L-kynurenine
-
Macaca fuscata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.60 mitochondrion
-
Rattus norvegicus 5739
-
2.6.1.60 mitochondrion
-
Macaca fuscata 5739
-
2.6.1.63 mitochondrion
-
Rattus norvegicus 5739
-
2.6.1.63 mitochondrion
-
Macaca fuscata 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.60 40000
-
2 * 40000, SDS-PAGE Rattus norvegicus
2.6.1.60 40000
-
2 * 40000, SDS-PAGE Macaca fuscata
2.6.1.60 80000
-
gel filtration Rattus norvegicus
2.6.1.60 80000
-
gel filtration Macaca fuscata
2.6.1.63 40000
-
2 * 40000, SDS-PAGE Rattus norvegicus
2.6.1.63 40000
-
2 * 40000, SDS-PAGE Macaca fuscata
2.6.1.63 80000
-
gel filtration Rattus norvegicus
2.6.1.63 80000
-
gel filtration Macaca fuscata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.60 an aromatic amino acid + glyoxylate Rattus norvegicus
-
an aromatic oxo acid + glycine
-
?
2.6.1.63 L-kynurenine + glyoxylate Rattus norvegicus glucagon inducible enzyme 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
-
?
2.6.1.63 L-kynurenine + glyoxylate Macaca fuscata glucagon inducible enzyme 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
-
?
2.6.1.63 L-kynurenine + glyoxylate Rattus norvegicus Donryu glucagon inducible enzyme 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.60 Macaca fuscata
-
Blyth monkey
-
2.6.1.60 Rattus norvegicus
-
-
-
2.6.1.63 Macaca fuscata
-
Blyth monkey
-
2.6.1.63 Rattus norvegicus
-
Donryu strain
-
2.6.1.63 Rattus norvegicus Donryu
-
Donryu strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.60
-
Rattus norvegicus
2.6.1.60 partial Macaca fuscata
2.6.1.63
-
Rattus norvegicus
2.6.1.63
-
Macaca fuscata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.60 liver
-
Rattus norvegicus
-
2.6.1.60 liver
-
Macaca fuscata
-
2.6.1.63 liver
-
Rattus norvegicus
-
2.6.1.63 liver
-
Macaca fuscata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.60 0.105
-
pH 7.5, 37°C Macaca fuscata
2.6.1.60 0.223
-
pH 7.5, 37°C Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.6.1.60 -20°C, at least 4 weeks Macaca fuscata
2.6.1.60 -20°C, at least 7 weeks Rattus norvegicus
2.6.1.60 0-4°C, 2 weeks with little or no loss of activity Macaca fuscata
2.6.1.60 0-4°C, 3 weeks with little or no loss of activity respectively Rattus norvegicus
2.6.1.63 -20°C, in 50 mM potassium phosphate buffer, pH 7.5, 0.2 mM pyridoxal 5’-phosphate, 5 mM 2-mercaptoethanol, 20% glycerol, at least 4 weeks Macaca fuscata
2.6.1.63 -20°C, in 50 mM potassium phosphate buffer, pH 7.5, 0.2 mM pyridoxal 5’-phosphate, 5 mM 2-mercaptoethanol, 20% glycerol, at least 7 weeks Rattus norvegicus
2.6.1.63 0-4°C, in 50 mM potassium phosphate buffer, pH 7.5, 0.2 mM pyridoxal 5’-phosphate, 5 mM 2-mercaptoethanol, 20% glycerol, 2 weeks Macaca fuscata
2.6.1.63 0-4°C, in 50 mM potassium phosphate buffer, pH 7.5, 0.2 mM pyridoxal phosphate, 5 mM mercaptoethanol, 20% glycerol, 3 weeks Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.60 5-hydroxytryptophan + glyoxylate less efficient amino group donor than L-phenylalanine Rattus norvegicus 3-(5-hydroxyindole)-2-oxopropanoate + glycine
-
?
2.6.1.60 5-hydroxytryptophan + glyoxylate less efficient amino group donor than L-phenylalanine Macaca fuscata 3-(5-hydroxyindole)-2-oxopropanoate + glycine
-
?
2.6.1.60 an aromatic amino acid + glyoxylate
-
Rattus norvegicus an aromatic oxo acid + glycine
-
?
2.6.1.60 L-histidine + glyoxylate
-
Rattus norvegicus 3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
-
?
2.6.1.60 L-histidine + glyoxylate
-
Macaca fuscata 3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
-
?
2.6.1.60 L-kynurenine + glyoxylate
-
Macaca fuscata 3-(2-aminophenyl-)2,4-dioxobutanoate + glycine
-
?
2.6.1.60 L-kynurenine + glyoxylate poor amino group donor Rattus norvegicus 3-(2-aminophenyl-)2,4-dioxobutanoate + glycine
-
?
2.6.1.60 L-phenylalanine + 2-oxo-4-methylthiobutyrate
-
Rattus norvegicus phenylpyruvate + L-methionine
-
?
2.6.1.60 L-phenylalanine + 2-oxo-4-methylthiobutyrate
-
Macaca fuscata phenylpyruvate + L-methionine
-
?
2.6.1.60 L-phenylalanine + 2-oxoglutarate
-
Rattus norvegicus phenylpyruvate + L-glutamate
-
?
2.6.1.60 L-phenylalanine + 2-oxoglutarate
-
Macaca fuscata phenylpyruvate + L-glutamate
-
?
2.6.1.60 L-phenylalanine + glyoxylate
-
Rattus norvegicus phenylpyruvate + glycine
-
?
2.6.1.60 L-phenylalanine + glyoxylate
-
Macaca fuscata phenylpyruvate + glycine
-
?
2.6.1.60 L-phenylalanine + hydroxypyruvate
-
Rattus norvegicus phenylpyruvate + L-serine
-
?
2.6.1.60 L-phenylalanine + hydroxypyruvate
-
Macaca fuscata phenylpyruvate + L-serine
-
?
2.6.1.60 L-phenylalanine + pyruvate
-
Rattus norvegicus phenylpyruvate + L-alanine
-
?
2.6.1.60 L-phenylalanine + pyruvate
-
Macaca fuscata phenylpyruvate + L-alanine
-
?
2.6.1.60 tryptophan + glyoxylate less efficient amino group donor than L-phenylalanine Rattus norvegicus 3-indole-2-oxopropanoate + glycine
-
?
2.6.1.60 tryptophan + glyoxylate less efficient amino group donor than L-phenylalanine Macaca fuscata 3-indole-2-oxopropanoate + glycine
-
?
2.6.1.60 tyrosine + glyoxylate
-
Rattus norvegicus 3-(4-hydroxyphenyl)-2-oxopropanoate + glycine
-
?
2.6.1.60 tyrosine + glyoxylate
-
Macaca fuscata 3-(4-hydroxyphenyl)-2-oxopropanoate + glycine
-
?
2.6.1.63 5-hydroxytryptophan + glyoxylate
-
Rattus norvegicus 3-(5-hydroxyindole)-2-oxopropanoate + glycine
-
?
2.6.1.63 5-hydroxytryptophan + glyoxylate
-
Macaca fuscata 3-(5-hydroxyindole)-2-oxopropanoate + glycine
-
?
2.6.1.63 histidine + glyoxylate
-
Rattus norvegicus 3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
-
?
2.6.1.63 histidine + glyoxylate
-
Macaca fuscata 3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
-
?
2.6.1.63 L-kynurenine + 2-oxoglutarate
-
Rattus norvegicus 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate
-
?
2.6.1.63 L-kynurenine + 4-methylsulfanyl-2-oxobutanoate
-
Rattus norvegicus 4-(2-aminophenyl)-2,4-dioxobutanoate + L-methionine
-
?
2.6.1.63 L-kynurenine + 4-methylsulfanyl-2-oxobutanoate
-
Macaca fuscata 4-(2-aminophenyl)-2,4-dioxobutanoate + L-methionine
-
?
2.6.1.63 L-kynurenine + 4-methylsulfanyl-2-oxobutanoate
-
Rattus norvegicus Donryu 4-(2-aminophenyl)-2,4-dioxobutanoate + L-methionine
-
?
2.6.1.63 L-kynurenine + glyoxylate
-
Rattus norvegicus 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
-
?
2.6.1.63 L-kynurenine + glyoxylate
-
Macaca fuscata 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
-
?
2.6.1.63 L-kynurenine + glyoxylate glucagon inducible enzyme Rattus norvegicus 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
-
?
2.6.1.63 L-kynurenine + glyoxylate glucagon inducible enzyme Macaca fuscata 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
-
?
2.6.1.63 L-kynurenine + glyoxylate
-
Rattus norvegicus Donryu 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
-
?
2.6.1.63 L-kynurenine + glyoxylate glucagon inducible enzyme Rattus norvegicus Donryu 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
-
?
2.6.1.63 L-kynurenine + hydroxypyruvate
-
Rattus norvegicus 4-(2-aminophenyl)-2,4-dioxobutanoate + L-serine
-
?
2.6.1.63 L-kynurenine + hydroxypyruvate
-
Macaca fuscata 4-(2-aminophenyl)-2,4-dioxobutanoate + L-serine
-
?
2.6.1.63 L-kynurenine + hydroxypyruvate
-
Rattus norvegicus Donryu 4-(2-aminophenyl)-2,4-dioxobutanoate + L-serine
-
?
2.6.1.63 L-kynurenine + pyruvate
-
Rattus norvegicus 4-(2-aminophenyl)-2,4-dioxobutanoate + L-alanine
-
?
2.6.1.63 L-kynurenine + pyruvate
-
Macaca fuscata 4-(2-aminophenyl)-2,4-dioxobutanoate + L-alanine
-
?
2.6.1.63 L-kynurenine + pyruvate
-
Rattus norvegicus Donryu 4-(2-aminophenyl)-2,4-dioxobutanoate + L-alanine
-
?
2.6.1.63 phenylalanine + glyoxylate
-
Rattus norvegicus phenylpyruvate + glycine
-
?
2.6.1.63 phenylalanine + glyoxylate
-
Macaca fuscata phenylpyruvate + glycine
-
?
2.6.1.63 tryptophan + glyoxylate
-
Rattus norvegicus 3-indole-2-oxopropanoate + glycine
-
?
2.6.1.63 tryptophan + glyoxylate
-
Macaca fuscata 3-indole-2-oxopropanoate + glycine
-
?
2.6.1.63 tyrosine + glyoxylate
-
Rattus norvegicus 3-(4-hydroxyphenyl)-2-oxopropanoate + glycine
-
?
2.6.1.63 tyrosine + glyoxylate
-
Macaca fuscata 3-(4-hydroxyphenyl)-2-oxopropanoate + glycine
-
?

Subunits

EC Number Subunits Comment Organism
2.6.1.60 dimer 2 * 40000, SDS-PAGE Rattus norvegicus
2.6.1.60 dimer 2 * 40000, SDS-PAGE Macaca fuscata
2.6.1.63 dimer 2 * 40000, SDS-PAGE Rattus norvegicus
2.6.1.63 dimer 2 * 40000, SDS-PAGE Macaca fuscata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.60 37
-
assay at Rattus norvegicus
2.6.1.60 37
-
assay at Macaca fuscata
2.6.1.63 37
-
assay at Rattus norvegicus
2.6.1.63 37
-
assay at Macaca fuscata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.63 70
-
t1/2: 25 min Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.60 additional information
-
pI: 8 Rattus norvegicus
2.6.1.60 additional information
-
pI: 8 Macaca fuscata
2.6.1.60 8 8.5
-
Rattus norvegicus
2.6.1.60 8 8.5
-
Macaca fuscata

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.60 pyridoxal 5'-phosphate requirement Rattus norvegicus
2.6.1.60 pyridoxal 5'-phosphate requirement Macaca fuscata
2.6.1.63 pyridoxal 5'-phosphate requirement, tightly bound Rattus norvegicus
2.6.1.63 pyridoxal 5'-phosphate requirement, tightly bound Macaca fuscata