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Literature summary extracted from

  • Nozaki, K.; Hamada, S.; Nakamori, T.; Ito, H.; Sagisaka, S.; Yoshida, H.; Takeda, Y.; Honma, M.; Matsui, H.
    Major isoforms of starch branching enzymes in premature seeds of kidney bean (Phaseolus vulgaris L.) (2001), Biosci. Biotechnol. Biochem., 65, 1141-1148.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.18 citrate activation Phaseolus vulgaris
2.4.1.18 citrate 3.3fold activation at 0.3 M, enzyme form KBE1 and KBE2 Phaseolus vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.18 CaCl2 10 mM, 50% inhibition. 100 mM, complete inactivation Phaseolus vulgaris
2.4.1.18 CuSO4 1 mM, complete inactivation Phaseolus vulgaris
2.4.1.18 HgCl2 1 mM, complete inactivation Phaseolus vulgaris
2.4.1.18 MgCl2 1 mM, complete inactivation Phaseolus vulgaris
2.4.1.18 MnCl2 1 mM, complete inactivation Phaseolus vulgaris
2.4.1.18 ZnCl2 1 mM, complete inactivation Phaseolus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.18 additional information
-
 additional information Km-value for amylose, enzyme form KBE1: 1.27 mg/ml. Km-value for amylose, enzyme form KBE2: 0.74 mg/ml Phaseolus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.18 77000
-
 1 * 77000, enzyme KBE2, SDS-PAGE Phaseolus vulgaris
2.4.1.18 80000
-
 1 * 80000, enzyme KBE1, SDS-PAGE Phaseolus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.18 Phaseolus vulgaris
-
 L. Toramame
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.18
-
 Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.18 seed developing seeds contain several isoforms. The major forms are KBE1 and KBE2 Phaseolus vulgaris
-

Subunits

EC Number Subunits Comment Organism
2.4.1.18 monomer 1 * 77000, enzyme KBE2, SDS-PAGE Phaseolus vulgaris
2.4.1.18 monomer 1 * 80000, enzyme KBE1, SDS-PAGE Phaseolus vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.18 25 30
-
 Phaseolus vulgaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.18 40
-
 pH 8.0, 15 min, stable up to, enzyme form KBE1 Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.18 7
-
-
 Phaseolus vulgaris

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.18 7 9.5 stable Phaseolus vulgaris
2.4.1.18 7 9.5 4°C, 16 h, less than 10% loss of activity, enzyme form KBE1 and KBE2 Phaseolus vulgaris