BRENDA - Enzyme Database

Kinetic and mutagenic characterization of the chromosomally encoded Salmonella enterica AAC(6')-Iy aminoglycoside N-acetyltransferase

Magnet, S.; Lambert, T.; Courvalin, P.; Blanchard, J.S.; Biochemistry 40, 3700-3709 (2001)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.3.1.82
-
Salmonella enterica
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.3.1.82
C109A
mutation neither abolishes activity nor alters the biphasic inactivation by iodoacetamide
Salmonella enterica
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.82
dithio-CoA
-
Salmonella enterica
2.3.1.82
iodoacetamide
inactivation in a biphasic manner, half of the activity is lost rapidly and the other half more slowly, tobramycin but not acetyl-CoA protects
Salmonella enterica
2.3.1.82
lividomycin
-
Salmonella enterica
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.82
0.006
-
netilmicin
mutant enzyme C109A
Salmonella enterica
2.3.1.82
0.008
-
netilmicin
wild-type enzyme
Salmonella enterica
2.3.1.82
0.01
-
acetyl-CoA
reaction with tobramycin
Salmonella enterica
2.3.1.82
0.012
-
sisomicin
wild-type enzyme
Salmonella enterica
2.3.1.82
0.014
-
neomycin C
wild-type enzyme
Salmonella enterica
2.3.1.82
0.017
-
acetyl-CoA
reaction with tobramycin, mutant enzyme C109A
Salmonella enterica
2.3.1.82
0.017
-
kanamycin B
mutant enzyme C109A
Salmonella enterica
2.3.1.82
0.026
-
tobramycin
mutant enzyme C109A
Salmonella enterica
2.3.1.82
0.03
-
dibekacin
wild-type enzyme
Salmonella enterica
2.3.1.82
0.05
-
amikacin
wild-type enzyme
Salmonella enterica
2.3.1.82
0.05
-
ribostamycin
wild-type enzyme
Salmonella enterica
2.3.1.82
0.066
-
tobramycin
wild-type enzyme
Salmonella enterica
2.3.1.82
0.07
-
acetyl-CoA
reaction with tobramycin, mutant enzyme C109A/C70A
Salmonella enterica
2.3.1.82
0.075
-
amikacin
mutant enzyme C109A
Salmonella enterica
2.3.1.82
0.079
-
kanamycin B
wild-type enzyme
Salmonella enterica
2.3.1.82
0.105
-
kanamycin A
wild-type enzyme
Salmonella enterica
2.3.1.82
0.25
-
neomycin C
mutant enzyme C109A/C70A
Salmonella enterica
2.3.1.82
0.27
-
propionyl-CoA
reaction with tobramycin, wild-type enzyme
Salmonella enterica
2.3.1.82
0.46
-
netilmicin
mutant enzyme C109A/C70A
Salmonella enterica
2.3.1.82
0.53
-
neomycin A
-
Salmonella enterica
2.3.1.82
0.54
-
tobramycin
mutant enzyme C109A/C70A
Salmonella enterica
2.3.1.82
0.82
-
malonyl-CoA
reaction with tobramycin, wild-type enzyme
Salmonella enterica
2.3.1.82
0.9
-
kanamycin B
mutant enzyme C109A/C70A
Salmonella enterica
2.3.1.82
9.3
-
butyryl-CoA
reaction with tobramycin, wild-type enzyme
Salmonella enterica
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.82
17000
-
2 * 17000, SDS-PAGE
Salmonella enterica
2.3.1.82
17173
-
2 * 17173, calculation from nucleotide sequence
Salmonella enterica
2.3.1.82
17184
-
2 * 17184, electrospray ionization mass spectrometry
Salmonella enterica
2.3.1.82
35000
-
gel filtration
Salmonella enterica
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.82
additional information
Salmonella enterica
involved in resistance to antibiotics
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.82
Salmonella enterica
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.82
acetyl-CoA + amikacin
-
636913
Salmonella enterica
CoA + N6'-acetylamikacin
-
-
-
?
2.3.1.82
acetyl-CoA + dibekacin
-
636913
Salmonella enterica
CoA + N6'-acetyldibekacin
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin A
-
636913
Salmonella enterica
CoA + N6'-acetylkanamycin A
-
636913
Salmonella enterica
?
2.3.1.82
acetyl-CoA + kanamycin B
-
636913
Salmonella enterica
CoA + N6'-acetylkanamycin B
-
-
-
?
2.3.1.82
acetyl-CoA + neomycin A
-
636913
Salmonella enterica
CoA + N6'-acetylneomycin A
-
-
-
?
2.3.1.82
acetyl-CoA + neomycin C
-
636913
Salmonella enterica
CoA + N6'-acetylneomycin C
-
-
-
?
2.3.1.82
acetyl-CoA + netilmicin
-
636913
Salmonella enterica
CoA + N6'-acetylnetilmicin
-
-
-
?
2.3.1.82
acetyl-CoA + ribostamycin
-
636913
Salmonella enterica
CoA + N6'-acetylribostamycin
-
-
-
?
2.3.1.82
acetyl-CoA + sisomicin
-
636913
Salmonella enterica
CoA + N6'-acetylsisomicin
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
636913
Salmonella enterica
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
butyryl-CoA + tobramycin
-
636913
Salmonella enterica
CoA + N6'-butyryltobramycin
-
-
-
?
2.3.1.82
malonyl-CoA + tobramycin
-
636913
Salmonella enterica
CoA + N6'-malonyltobramycin
-
-
-
?
2.3.1.82
additional information
involved in resistance to antibiotics
636913
Salmonella enterica
?
-
-
-
-
2.3.1.82
propionyl-CoA + tobramycin
tobramycin exhibits a rapid, tobramycin-independent rate of hydrolysis that is linearly proportional to enzyme
636913
Salmonella enterica
CoA + N6'-propionyltobramycin
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.82
dimer
2 * 17000, SDS-PAGE; 2 * 17173, calculation from nucleotide sequence; 2 * 17184, electrospray ionization mass spectrometry
Salmonella enterica
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.82
-
Salmonella enterica
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.3.1.82
C109A
mutation neither abolishes activity nor alters the biphasic inactivation by iodoacetamide
Salmonella enterica
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.82
dithio-CoA
-
Salmonella enterica
2.3.1.82
iodoacetamide
inactivation in a biphasic manner, half of the activity is lost rapidly and the other half more slowly, tobramycin but not acetyl-CoA protects
Salmonella enterica
2.3.1.82
lividomycin
-
Salmonella enterica
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.82
0.006
-
netilmicin
mutant enzyme C109A
Salmonella enterica
2.3.1.82
0.008
-
netilmicin
wild-type enzyme
Salmonella enterica
2.3.1.82
0.01
-
acetyl-CoA
reaction with tobramycin
Salmonella enterica
2.3.1.82
0.012
-
sisomicin
wild-type enzyme
Salmonella enterica
2.3.1.82
0.014
-
neomycin C
wild-type enzyme
Salmonella enterica
2.3.1.82
0.017
-
acetyl-CoA
reaction with tobramycin, mutant enzyme C109A
Salmonella enterica
2.3.1.82
0.017
-
kanamycin B
mutant enzyme C109A
Salmonella enterica
2.3.1.82
0.026
-
tobramycin
mutant enzyme C109A
Salmonella enterica
2.3.1.82
0.03
-
dibekacin
wild-type enzyme
Salmonella enterica
2.3.1.82
0.05
-
amikacin
wild-type enzyme
Salmonella enterica
2.3.1.82
0.05
-
ribostamycin
wild-type enzyme
Salmonella enterica
2.3.1.82
0.066
-
tobramycin
wild-type enzyme
Salmonella enterica
2.3.1.82
0.07
-
acetyl-CoA
reaction with tobramycin, mutant enzyme C109A/C70A
Salmonella enterica
2.3.1.82
0.075
-
amikacin
mutant enzyme C109A
Salmonella enterica
2.3.1.82
0.079
-
kanamycin B
wild-type enzyme
Salmonella enterica
2.3.1.82
0.105
-
kanamycin A
wild-type enzyme
Salmonella enterica
2.3.1.82
0.25
-
neomycin C
mutant enzyme C109A/C70A
Salmonella enterica
2.3.1.82
0.27
-
propionyl-CoA
reaction with tobramycin, wild-type enzyme
Salmonella enterica
2.3.1.82
0.46
-
netilmicin
mutant enzyme C109A/C70A
Salmonella enterica
2.3.1.82
0.53
-
neomycin A
-
Salmonella enterica
2.3.1.82
0.54
-
tobramycin
mutant enzyme C109A/C70A
Salmonella enterica
2.3.1.82
0.82
-
malonyl-CoA
reaction with tobramycin, wild-type enzyme
Salmonella enterica
2.3.1.82
0.9
-
kanamycin B
mutant enzyme C109A/C70A
Salmonella enterica
2.3.1.82
9.3
-
butyryl-CoA
reaction with tobramycin, wild-type enzyme
Salmonella enterica
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.82
17000
-
2 * 17000, SDS-PAGE
Salmonella enterica
2.3.1.82
17173
-
2 * 17173, calculation from nucleotide sequence
Salmonella enterica
2.3.1.82
17184
-
2 * 17184, electrospray ionization mass spectrometry
Salmonella enterica
2.3.1.82
35000
-
gel filtration
Salmonella enterica
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.82
additional information
Salmonella enterica
involved in resistance to antibiotics
?
-
-
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.82
acetyl-CoA + amikacin
-
636913
Salmonella enterica
CoA + N6'-acetylamikacin
-
-
-
?
2.3.1.82
acetyl-CoA + dibekacin
-
636913
Salmonella enterica
CoA + N6'-acetyldibekacin
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin A
-
636913
Salmonella enterica
CoA + N6'-acetylkanamycin A
-
636913
Salmonella enterica
?
2.3.1.82
acetyl-CoA + kanamycin B
-
636913
Salmonella enterica
CoA + N6'-acetylkanamycin B
-
-
-
?
2.3.1.82
acetyl-CoA + neomycin A
-
636913
Salmonella enterica
CoA + N6'-acetylneomycin A
-
-
-
?
2.3.1.82
acetyl-CoA + neomycin C
-
636913
Salmonella enterica
CoA + N6'-acetylneomycin C
-
-
-
?
2.3.1.82
acetyl-CoA + netilmicin
-
636913
Salmonella enterica
CoA + N6'-acetylnetilmicin
-
-
-
?
2.3.1.82
acetyl-CoA + ribostamycin
-
636913
Salmonella enterica
CoA + N6'-acetylribostamycin
-
-
-
?
2.3.1.82
acetyl-CoA + sisomicin
-
636913
Salmonella enterica
CoA + N6'-acetylsisomicin
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
636913
Salmonella enterica
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
butyryl-CoA + tobramycin
-
636913
Salmonella enterica
CoA + N6'-butyryltobramycin
-
-
-
?
2.3.1.82
malonyl-CoA + tobramycin
-
636913
Salmonella enterica
CoA + N6'-malonyltobramycin
-
-
-
?
2.3.1.82
additional information
involved in resistance to antibiotics
636913
Salmonella enterica
?
-
-
-
-
2.3.1.82
propionyl-CoA + tobramycin
tobramycin exhibits a rapid, tobramycin-independent rate of hydrolysis that is linearly proportional to enzyme
636913
Salmonella enterica
CoA + N6'-propionyltobramycin
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.82
dimer
2 * 17000, SDS-PAGE; 2 * 17173, calculation from nucleotide sequence; 2 * 17184, electrospray ionization mass spectrometry
Salmonella enterica