BRENDA - Enzyme Database

pH Modulation of aminoglycoside resistance in Staphylococcus epidermidis harboring 6'-N-aminoglycoside acetyltransferase

Culebras, E.; Martinez, J.L.; Baquero, F.; Perez-Diaz, J.C.; J. Antimicrob. Chemother. 37, 881-889 (1996)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.82
amikacin
substrate inhibition
Staphylococcus epidermidis
2.3.1.82
gentamicin
substrate inhibition
Staphylococcus epidermidis
2.3.1.82
netilmicin
substrate inhibition
Staphylococcus epidermidis
2.3.1.82
tobramycin
substrate inhibition
Staphylococcus epidermidis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.82
0.026
-
tobramycin
pH 7.5, 37°C
Staphylococcus epidermidis
2.3.1.82
0.036
-
amikacin
pH 7.5, 37°C
Staphylococcus epidermidis
2.3.1.82
0.077
-
gentamicin
pH 7.5, 37°C
Staphylococcus epidermidis
2.3.1.82
0.077
-
tobramycin
pH 6.5, 37°C
Staphylococcus epidermidis
2.3.1.82
0.216
-
tobramicin
pH 5.5, 37°C
Staphylococcus epidermidis
2.3.1.82
0.259
-
netilmicin
pH 7.5, 37°C
Staphylococcus epidermidis
2.3.1.82
0.297
-
gentamicin
pH 6.5, 37°C
Staphylococcus epidermidis
2.3.1.82
0.364
-
amikacin
pH 6.5, 37°C
Staphylococcus epidermidis
2.3.1.82
0.571
-
amikacin
pH 5.5, 37°C
Staphylococcus epidermidis
2.3.1.82
0.653
-
gentamicin
pH 5.5, 37°C
Staphylococcus epidermidis
2.3.1.82
2.44
-
netilmicin
pH 6.5, 37°C
Staphylococcus epidermidis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.82
additional information
Staphylococcus epidermidis
involved in resistance to antibiotics
?
-
-
-
2.3.1.82
additional information
Staphylococcus epidermidis RYC 13036
involved in resistance to antibiotics
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.82
Staphylococcus epidermidis
-
RYC 13036
-
2.3.1.82
Staphylococcus epidermidis RYC 13036
-
RYC 13036
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.82
acetyl-CoA + amikacin
-
636905
Staphylococcus epidermidis
CoA + N6'-acetylamikacin
-
-
-
?
2.3.1.82
acetyl-CoA + amikacin
-
636905
Staphylococcus epidermidis RYC 13036
CoA + N6'-acetylamikacin
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin
-
636905
Staphylococcus epidermidis
CoA + N6'-acetylgentamicin
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin
-
636905
Staphylococcus epidermidis RYC 13036
CoA + N6'-acetylgentamicin
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin C
-
636905
Staphylococcus epidermidis
CoA + N6'-acetylgentamicin C
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin C
-
636905
Staphylococcus epidermidis RYC 13036
CoA + N6'-acetylgentamicin C
-
-
-
?
2.3.1.82
acetyl-CoA + netilmicin
-
636905
Staphylococcus epidermidis
CoA + N6'-acetylnetilmicin
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
636905
Staphylococcus epidermidis
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
636905
Staphylococcus epidermidis RYC 13036
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
additional information
involved in resistance to antibiotics
636905
Staphylococcus epidermidis
?
-
-
-
-
2.3.1.82
additional information
involved in resistance to antibiotics
636905
Staphylococcus epidermidis RYC 13036
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.82
6.5
-
reaction with tobramycin, amikacin or gentamicin
Staphylococcus epidermidis
2.3.1.82
7.5
-
activity with netilmicin
Staphylococcus epidermidis
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.3.1.82
0.00059
-
tobramycin
pH 7.5, 37°C, substrate inhibition
Staphylococcus epidermidis
2.3.1.82
0.0023
-
tobramycin
pH 6.5, 37°C, substrate inhibition
Staphylococcus epidermidis
2.3.1.82
0.00499
-
amikacin
pH 7.5, 37°C, substrate inhibition
Staphylococcus epidermidis
2.3.1.82
0.00647
-
tobramycin
pH 5.5, 37°C, substrate inhibition
Staphylococcus epidermidis
2.3.1.82
0.0087
-
amikacin
pH 6.5, 37°C, substrate inhibition
Staphylococcus epidermidis
2.3.1.82
0.02206
-
amikacin
pH 5.5, 37°C, substrate inhibition
Staphylococcus epidermidis
2.3.1.82
0.02256
-
gentamicin
pH 5.5, 37°C, substrate inhibition
Staphylococcus epidermidis
2.3.1.82
0.0482
-
gentamicin
pH 6.5, 37°C, substrate inhibition
Staphylococcus epidermidis
2.3.1.82
0.06446
-
netilmicin
pH 5.5, 37°C, substrate inhibition
Staphylococcus epidermidis
2.3.1.82
0.075
-
netilmicin
pH 6.5, 37°C, substrate inhibition
Staphylococcus epidermidis
2.3.1.82
0.079
-
gentamicin
pH 7.5, 37°C, substrate inhibition
Staphylococcus epidermidis
2.3.1.82
0.168
-
netilmicin
pH 7.5, 37°C, substrate inhibition
Staphylococcus epidermidis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.82
amikacin
substrate inhibition
Staphylococcus epidermidis
2.3.1.82
gentamicin
substrate inhibition
Staphylococcus epidermidis
2.3.1.82
netilmicin
substrate inhibition
Staphylococcus epidermidis
2.3.1.82
tobramycin
substrate inhibition
Staphylococcus epidermidis
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.3.1.82
0.00059
-
tobramycin
pH 7.5, 37°C, substrate inhibition
Staphylococcus epidermidis
2.3.1.82
0.0023
-
tobramycin
pH 6.5, 37°C, substrate inhibition
Staphylococcus epidermidis
2.3.1.82
0.00499
-
amikacin
pH 7.5, 37°C, substrate inhibition
Staphylococcus epidermidis
2.3.1.82
0.00647
-
tobramycin
pH 5.5, 37°C, substrate inhibition
Staphylococcus epidermidis
2.3.1.82
0.0087
-
amikacin
pH 6.5, 37°C, substrate inhibition
Staphylococcus epidermidis
2.3.1.82
0.02206
-
amikacin
pH 5.5, 37°C, substrate inhibition
Staphylococcus epidermidis
2.3.1.82
0.02256
-
gentamicin
pH 5.5, 37°C, substrate inhibition
Staphylococcus epidermidis
2.3.1.82
0.0482
-
gentamicin
pH 6.5, 37°C, substrate inhibition
Staphylococcus epidermidis
2.3.1.82
0.06446
-
netilmicin
pH 5.5, 37°C, substrate inhibition
Staphylococcus epidermidis
2.3.1.82
0.075
-
netilmicin
pH 6.5, 37°C, substrate inhibition
Staphylococcus epidermidis
2.3.1.82
0.079
-
gentamicin
pH 7.5, 37°C, substrate inhibition
Staphylococcus epidermidis
2.3.1.82
0.168
-
netilmicin
pH 7.5, 37°C, substrate inhibition
Staphylococcus epidermidis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.82
0.026
-
tobramycin
pH 7.5, 37°C
Staphylococcus epidermidis
2.3.1.82
0.036
-
amikacin
pH 7.5, 37°C
Staphylococcus epidermidis
2.3.1.82
0.077
-
gentamicin
pH 7.5, 37°C
Staphylococcus epidermidis
2.3.1.82
0.077
-
tobramycin
pH 6.5, 37°C
Staphylococcus epidermidis
2.3.1.82
0.216
-
tobramicin
pH 5.5, 37°C
Staphylococcus epidermidis
2.3.1.82
0.259
-
netilmicin
pH 7.5, 37°C
Staphylococcus epidermidis
2.3.1.82
0.297
-
gentamicin
pH 6.5, 37°C
Staphylococcus epidermidis
2.3.1.82
0.364
-
amikacin
pH 6.5, 37°C
Staphylococcus epidermidis
2.3.1.82
0.571
-
amikacin
pH 5.5, 37°C
Staphylococcus epidermidis
2.3.1.82
0.653
-
gentamicin
pH 5.5, 37°C
Staphylococcus epidermidis
2.3.1.82
2.44
-
netilmicin
pH 6.5, 37°C
Staphylococcus epidermidis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.82
additional information
Staphylococcus epidermidis
involved in resistance to antibiotics
?
-
-
-
2.3.1.82
additional information
Staphylococcus epidermidis RYC 13036
involved in resistance to antibiotics
?
-
-
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.82
acetyl-CoA + amikacin
-
636905
Staphylococcus epidermidis
CoA + N6'-acetylamikacin
-
-
-
?
2.3.1.82
acetyl-CoA + amikacin
-
636905
Staphylococcus epidermidis RYC 13036
CoA + N6'-acetylamikacin
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin
-
636905
Staphylococcus epidermidis
CoA + N6'-acetylgentamicin
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin
-
636905
Staphylococcus epidermidis RYC 13036
CoA + N6'-acetylgentamicin
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin C
-
636905
Staphylococcus epidermidis
CoA + N6'-acetylgentamicin C
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin C
-
636905
Staphylococcus epidermidis RYC 13036
CoA + N6'-acetylgentamicin C
-
-
-
?
2.3.1.82
acetyl-CoA + netilmicin
-
636905
Staphylococcus epidermidis
CoA + N6'-acetylnetilmicin
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
636905
Staphylococcus epidermidis
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
636905
Staphylococcus epidermidis RYC 13036
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
additional information
involved in resistance to antibiotics
636905
Staphylococcus epidermidis
?
-
-
-
-
2.3.1.82
additional information
involved in resistance to antibiotics
636905
Staphylococcus epidermidis RYC 13036
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.82
6.5
-
reaction with tobramycin, amikacin or gentamicin
Staphylococcus epidermidis
2.3.1.82
7.5
-
activity with netilmicin
Staphylococcus epidermidis