BRENDA - Enzyme Database

Kinetic studies of aminoglycoside acetyltransferase and phosphotransferase from Staphylococcus aureus RPAL. Relationship between the two activities

Martel, A.; Masson, M.; Moreau, N.; Le Goffic, F.; Eur. J. Biochem. 133, 515-521 (1983)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.3.1.82
GTP, CoA, gentamicin C, neamine protect against thermal inactivation
Staphylococcus aureus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.82
6'-N-acetylneamine
product inhibition
Staphylococcus aureus
2.3.1.82
AMP
-
Staphylococcus aureus
2.3.1.82
CoA
product inhibition
Staphylococcus aureus
2.3.1.82
gentamicin C1
competitive inhibitor of neamine, non-competitive inhibitor of acetyl-CoA
Staphylococcus aureus
2.3.1.82
additional information
no inhibition by GTP
Staphylococcus aureus
2.3.1.82
paromomycin
inhibits reaction with neamine
Staphylococcus aureus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.82
55000
-
sucrose density gradient centrifugation
Staphylococcus aureus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.3.1.82
additional information
Staphylococcus aureus
involved in resistance to antibiotics
?
-
-
-
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.3.1.82
Staphylococcus aureus
-
harbouring plasmid RPAL
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.3.1.82
unseparable from 2-O-aminoglycoside phosphotransferase by affinity chromatography or sucrose density gradient ultracentrifugation
Staphylococcus aureus
Reaction
EC Number
Reaction
Commentary
Organism
Reaction ID
2.3.1.82
acetyl-CoA + kanamycin-B = CoA + N6'-acetylkanamycin-B
mechanism
Staphylococcus aureus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.82
0.304
-
-
Staphylococcus aureus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.3.1.82
acetyl-CoA + gentamicin C1a
-
636902
Staphylococcus aureus
CoA + N6'-acetylgentamicin C1a
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin B
-
636902
Staphylococcus aureus
CoA + N6'-acetylkanamycin B
-
-
-
?
2.3.1.82
acetyl-CoA + neamine
-
636902
Staphylococcus aureus
CoA + 6'-N-acetylneamine
-
636902
Staphylococcus aureus
?
2.3.1.82
acetyl-CoA + neamine
-
636902
Staphylococcus aureus
CoA + N6'-acetylneamine
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
636902
Staphylococcus aureus
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
additional information
involved in resistance to antibiotics
636902
Staphylococcus aureus
?
-
-
-
-
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.3.1.82
additional information
-
GTP, CoA, gentamicin C, neamine protect against thermal inactivation
Staphylococcus aureus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.3.1.82
0.0036
-
CoA
pH 6.9, 37°C
Staphylococcus aureus
2.3.1.82
4
-
gentamicin C1
pH 6.9, 37°C, inhibition of the reaction with neamine
Staphylococcus aureus
2.3.1.82
6
-
AMP
pH 6.9, 37°C, inhibition of the reaction with neamine
Staphylococcus aureus
2.3.1.82
7.7
-
paromomycin
pH 6.9, 37°C, inhibition of the reaction with neamine
Staphylococcus aureus
General Stability (protein specific)
EC Number
General Stability
Organism
2.3.1.82
GTP, CoA, gentamicin C, neamine protect against thermal inactivation
Staphylococcus aureus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.82
6'-N-acetylneamine
product inhibition
Staphylococcus aureus
2.3.1.82
AMP
-
Staphylococcus aureus
2.3.1.82
CoA
product inhibition
Staphylococcus aureus
2.3.1.82
gentamicin C1
competitive inhibitor of neamine, non-competitive inhibitor of acetyl-CoA
Staphylococcus aureus
2.3.1.82
additional information
no inhibition by GTP
Staphylococcus aureus
2.3.1.82
paromomycin
inhibits reaction with neamine
Staphylococcus aureus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.3.1.82
0.0036
-
CoA
pH 6.9, 37°C
Staphylococcus aureus
2.3.1.82
4
-
gentamicin C1
pH 6.9, 37°C, inhibition of the reaction with neamine
Staphylococcus aureus
2.3.1.82
6
-
AMP
pH 6.9, 37°C, inhibition of the reaction with neamine
Staphylococcus aureus
2.3.1.82
7.7
-
paromomycin
pH 6.9, 37°C, inhibition of the reaction with neamine
Staphylococcus aureus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.82
55000
-
sucrose density gradient centrifugation
Staphylococcus aureus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.3.1.82
additional information
Staphylococcus aureus
involved in resistance to antibiotics
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.82
unseparable from 2-O-aminoglycoside phosphotransferase by affinity chromatography or sucrose density gradient ultracentrifugation
Staphylococcus aureus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.82
0.304
-
-
Staphylococcus aureus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.3.1.82
acetyl-CoA + gentamicin C1a
-
636902
Staphylococcus aureus
CoA + N6'-acetylgentamicin C1a
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin B
-
636902
Staphylococcus aureus
CoA + N6'-acetylkanamycin B
-
-
-
?
2.3.1.82
acetyl-CoA + neamine
-
636902
Staphylococcus aureus
CoA + 6'-N-acetylneamine
-
636902
Staphylococcus aureus
?
2.3.1.82
acetyl-CoA + neamine
-
636902
Staphylococcus aureus
CoA + N6'-acetylneamine
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
636902
Staphylococcus aureus
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
additional information
involved in resistance to antibiotics
636902
Staphylococcus aureus
?
-
-
-
-
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.3.1.82
additional information
-
GTP, CoA, gentamicin C, neamine protect against thermal inactivation
Staphylococcus aureus