BRENDA - Enzyme Database

Characterization of aminoglycoside 6-N-acetyltransferases [AAC(6)] from gram-negative bacteria and Streptomyces kanamyceticus

Meyer, J.F.; Wiedemann, B.; J. Antimicrob. Chemother. 15, 271-282 (1985)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
2.3.1.82
Pseudomonas aeruginosa 141, gene transfer via plasmid pBP30 to Escherichia coli Hb101
Escherichia coli
2.3.1.82
Pseudomonas aeruginosa 141, gene transfer via plasmid pBP30 to Escherichia coli Hb101
Pseudomonas aeruginosa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.82
0.001
-
kanamycin A
pH 6.0
Pseudomonas aeruginosa
2.3.1.82
0.001
-
kanamycin A
pH 7.5
Serratia marcescens
2.3.1.82
0.005
-
kanamycin A
pH 6.0
Pseudomonas aeruginosa
2.3.1.82
0.006
-
kanamycin A
pH 7.5
Serratia marcescens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.82
55000
-
x * 55000, strain GN315, SDS-PAGE
Serratia marcescens
2.3.1.82
57000
-
x * 57000, SDS-PAGE
Serratia marcescens
2.3.1.82
59000
-
x * 59000, strain 141, SDS-PAGE
Pseudomonas aeruginosa
2.3.1.82
75000
-
x * 75000, SDS-PAGE
Streptomyces kanamyceticus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.3.1.82
additional information
Escherichia coli
involved in resistance to antibiotics
?
-
-
-
2.3.1.82
additional information
Pseudomonas aeruginosa
involved in resistance to antibiotics
?
-
-
-
2.3.1.82
additional information
Serratia marcescens
involved in resistance to antibiotics
?
-
-
-
2.3.1.82
additional information
Streptomyces kanamyceticus
involved in resistance to antibiotics
?
-
-
-
2.3.1.82
additional information
Escherichia coli NR79
involved in resistance to antibiotics
?
-
-
-
2.3.1.82
additional information
Serratia marcescens VU12944/77
involved in resistance to antibiotics
?
-
-
-
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.3.1.82
Escherichia coli
-
NR79
-
2.3.1.82
Escherichia coli NR79
-
NR79
-
2.3.1.82
Pseudomonas aeruginosa
-
strains GN315 and 141
-
2.3.1.82
Serratia marcescens
-
-
-
2.3.1.82
Serratia marcescens VU12944/77
-
-
-
2.3.1.82
Streptomyces kanamyceticus
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.3.1.82
-
Escherichia coli
2.3.1.82
-
Serratia marcescens
2.3.1.82
-
Streptomyces kanamyceticus
2.3.1.82
strain GN315
Pseudomonas aeruginosa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.3.1.82
acetyl-CoA + amikacin
-
636901
Escherichia coli
CoA + N6'-acetylamikacin
-
-
-
?
2.3.1.82
acetyl-CoA + amikacin
-
636901
Pseudomonas aeruginosa
CoA + N6'-acetylamikacin
-
-
-
?
2.3.1.82
acetyl-CoA + amikacin
-
636901
Serratia marcescens
CoA + N6'-acetylamikacin
-
-
-
?
2.3.1.82
acetyl-CoA + amikacin
-
636901
Streptomyces kanamyceticus
CoA + N6'-acetylamikacin
-
-
-
?
2.3.1.82
acetyl-CoA + dibekacin
-
636901
Escherichia coli
CoA + N6'-acetyldibekacin
-
-
-
?
2.3.1.82
acetyl-CoA + dibekacin
-
636901
Pseudomonas aeruginosa
CoA + N6'-acetyldibekacin
-
-
-
?
2.3.1.82
acetyl-CoA + dibekacin
-
636901
Serratia marcescens
CoA + N6'-acetyldibekacin
-
-
-
?
2.3.1.82
acetyl-CoA + dibekacin
-
636901
Streptomyces kanamyceticus
CoA + N6'-acetyldibekacin
-
-
-
?
2.3.1.82
acetyl-CoA + dibekacin
-
636901
Escherichia coli NR79
CoA + N6'-acetyldibekacin
-
-
-
?
2.3.1.82
acetyl-CoA + dibekacin
-
636901
Serratia marcescens VU12944/77
CoA + N6'-acetyldibekacin
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin C1a
-
636901
Escherichia coli
CoA + N6'-acetylgentamicin C1a
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin C1a
-
636901
Pseudomonas aeruginosa
CoA + N6'-acetylgentamicin C1a
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin C1a
-
636901
Serratia marcescens
CoA + N6'-acetylgentamicin C1a
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin C1a
-
636901
Streptomyces kanamyceticus
CoA + N6'-acetylgentamicin C1a
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin C1a
-
636901
Escherichia coli NR79
CoA + N6'-acetylgentamicin C1a
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin C1a
-
636901
Serratia marcescens VU12944/77
CoA + N6'-acetylgentamicin C1a
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin A
-
636901
Escherichia coli
CoA + N6'-acetylkanamycin A
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin A
-
636901
Pseudomonas aeruginosa
CoA + N6'-acetylkanamycin A
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin A
-
636901
Serratia marcescens
CoA + N6'-acetylkanamycin A
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin A
-
636901
Streptomyces kanamyceticus
CoA + N6'-acetylkanamycin A
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin A
-
636901
Escherichia coli NR79
CoA + N6'-acetylkanamycin A
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin A
-
636901
Serratia marcescens VU12944/77
CoA + N6'-acetylkanamycin A
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
636901
Escherichia coli
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
636901
Serratia marcescens
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
636901
Streptomyces kanamyceticus
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
636901
Escherichia coli NR79
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
636901
Serratia marcescens VU12944/77
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
additional information
involved in resistance to antibiotics
636901
Escherichia coli
?
-
-
-
-
2.3.1.82
additional information
involved in resistance to antibiotics
636901
Pseudomonas aeruginosa
?
-
-
-
-
2.3.1.82
additional information
involved in resistance to antibiotics
636901
Serratia marcescens
?
-
-
-
-
2.3.1.82
additional information
involved in resistance to antibiotics
636901
Streptomyces kanamyceticus
?
-
-
-
-
2.3.1.82
additional information
involved in resistance to antibiotics
636901
Escherichia coli NR79
?
-
-
-
-
2.3.1.82
additional information
involved in resistance to antibiotics
636901
Serratia marcescens VU12944/77
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.82
?
x * 59000, strain 141, SDS-PAGE
Pseudomonas aeruginosa
2.3.1.82
?
x * 55000, strain GN315, SDS-PAGE; x * 57000, SDS-PAGE
Serratia marcescens
2.3.1.82
?
x * 75000, SDS-PAGE
Streptomyces kanamyceticus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.82
6
-
-
Escherichia coli
2.3.1.82
6
-
-
Pseudomonas aeruginosa
2.3.1.82
6.5
-
-
Pseudomonas aeruginosa
2.3.1.82
7
-
-
Streptomyces kanamyceticus
2.3.1.82
7.5
-
-
Serratia marcescens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.82
Pseudomonas aeruginosa 141, gene transfer via plasmid pBP30 to Escherichia coli Hb101
Escherichia coli
2.3.1.82
Pseudomonas aeruginosa 141, gene transfer via plasmid pBP30 to Escherichia coli Hb101
Pseudomonas aeruginosa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.82
0.001
-
kanamycin A
pH 6.0
Pseudomonas aeruginosa
2.3.1.82
0.001
-
kanamycin A
pH 7.5
Serratia marcescens
2.3.1.82
0.005
-
kanamycin A
pH 6.0
Pseudomonas aeruginosa
2.3.1.82
0.006
-
kanamycin A
pH 7.5
Serratia marcescens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.82
55000
-
x * 55000, strain GN315, SDS-PAGE
Serratia marcescens
2.3.1.82
57000
-
x * 57000, SDS-PAGE
Serratia marcescens
2.3.1.82
59000
-
x * 59000, strain 141, SDS-PAGE
Pseudomonas aeruginosa
2.3.1.82
75000
-
x * 75000, SDS-PAGE
Streptomyces kanamyceticus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.3.1.82
additional information
Escherichia coli
involved in resistance to antibiotics
?
-
-
-
2.3.1.82
additional information
Pseudomonas aeruginosa
involved in resistance to antibiotics
?
-
-
-
2.3.1.82
additional information
Serratia marcescens
involved in resistance to antibiotics
?
-
-
-
2.3.1.82
additional information
Streptomyces kanamyceticus
involved in resistance to antibiotics
?
-
-
-
2.3.1.82
additional information
Escherichia coli NR79
involved in resistance to antibiotics
?
-
-
-
2.3.1.82
additional information
Serratia marcescens VU12944/77
involved in resistance to antibiotics
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.82
-
Escherichia coli
2.3.1.82
-
Serratia marcescens
2.3.1.82
-
Streptomyces kanamyceticus
2.3.1.82
strain GN315
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.3.1.82
acetyl-CoA + amikacin
-
636901
Escherichia coli
CoA + N6'-acetylamikacin
-
-
-
?
2.3.1.82
acetyl-CoA + amikacin
-
636901
Pseudomonas aeruginosa
CoA + N6'-acetylamikacin
-
-
-
?
2.3.1.82
acetyl-CoA + amikacin
-
636901
Serratia marcescens
CoA + N6'-acetylamikacin
-
-
-
?
2.3.1.82
acetyl-CoA + amikacin
-
636901
Streptomyces kanamyceticus
CoA + N6'-acetylamikacin
-
-
-
?
2.3.1.82
acetyl-CoA + dibekacin
-
636901
Escherichia coli
CoA + N6'-acetyldibekacin
-
-
-
?
2.3.1.82
acetyl-CoA + dibekacin
-
636901
Pseudomonas aeruginosa
CoA + N6'-acetyldibekacin
-
-
-
?
2.3.1.82
acetyl-CoA + dibekacin
-
636901
Serratia marcescens
CoA + N6'-acetyldibekacin
-
-
-
?
2.3.1.82
acetyl-CoA + dibekacin
-
636901
Streptomyces kanamyceticus
CoA + N6'-acetyldibekacin
-
-
-
?
2.3.1.82
acetyl-CoA + dibekacin
-
636901
Escherichia coli NR79
CoA + N6'-acetyldibekacin
-
-
-
?
2.3.1.82
acetyl-CoA + dibekacin
-
636901
Serratia marcescens VU12944/77
CoA + N6'-acetyldibekacin
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin C1a
-
636901
Escherichia coli
CoA + N6'-acetylgentamicin C1a
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin C1a
-
636901
Pseudomonas aeruginosa
CoA + N6'-acetylgentamicin C1a
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin C1a
-
636901
Serratia marcescens
CoA + N6'-acetylgentamicin C1a
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin C1a
-
636901
Streptomyces kanamyceticus
CoA + N6'-acetylgentamicin C1a
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin C1a
-
636901
Escherichia coli NR79
CoA + N6'-acetylgentamicin C1a
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin C1a
-
636901
Serratia marcescens VU12944/77
CoA + N6'-acetylgentamicin C1a
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin A
-
636901
Escherichia coli
CoA + N6'-acetylkanamycin A
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin A
-
636901
Pseudomonas aeruginosa
CoA + N6'-acetylkanamycin A
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin A
-
636901
Serratia marcescens
CoA + N6'-acetylkanamycin A
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin A
-
636901
Streptomyces kanamyceticus
CoA + N6'-acetylkanamycin A
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin A
-
636901
Escherichia coli NR79
CoA + N6'-acetylkanamycin A
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin A
-
636901
Serratia marcescens VU12944/77
CoA + N6'-acetylkanamycin A
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
636901
Escherichia coli
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
636901
Serratia marcescens
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
636901
Streptomyces kanamyceticus
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
636901
Escherichia coli NR79
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
636901
Serratia marcescens VU12944/77
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
additional information
involved in resistance to antibiotics
636901
Escherichia coli
?
-
-
-
-
2.3.1.82
additional information
involved in resistance to antibiotics
636901
Pseudomonas aeruginosa
?
-
-
-
-
2.3.1.82
additional information
involved in resistance to antibiotics
636901
Serratia marcescens
?
-
-
-
-
2.3.1.82
additional information
involved in resistance to antibiotics
636901
Streptomyces kanamyceticus
?
-
-
-
-
2.3.1.82
additional information
involved in resistance to antibiotics
636901
Escherichia coli NR79
?
-
-
-
-
2.3.1.82
additional information
involved in resistance to antibiotics
636901
Serratia marcescens VU12944/77
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.82
?
x * 59000, strain 141, SDS-PAGE
Pseudomonas aeruginosa
2.3.1.82
?
x * 55000, strain GN315, SDS-PAGE; x * 57000, SDS-PAGE
Serratia marcescens
2.3.1.82
?
x * 75000, SDS-PAGE
Streptomyces kanamyceticus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.82
6
-
-
Escherichia coli
2.3.1.82
6
-
-
Pseudomonas aeruginosa
2.3.1.82
6.5
-
-
Pseudomonas aeruginosa
2.3.1.82
7
-
-
Streptomyces kanamyceticus
2.3.1.82
7.5
-
-
Serratia marcescens