BRENDA - Enzyme Database

Resistance to aminosides induced by an isoenzyme, kanamycin acetyltransferase

Le Goffic, F.; Martel, A.; Biochimie 56, 893-897 (1974)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.82
gentamicin C1
-
Moraxella sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.82
0.004
-
tobramycin
-
Escherichia coli
2.3.1.82
0.004
-
tobramycin
-
Moraxella sp.
2.3.1.82
0.008
-
kanamycin
-
Moraxella sp.
2.3.1.82
0.022
-
tobramycin
-
Escherichia coli
2.3.1.82
0.057
-
amikacin
-
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.3.1.82
additional information
Escherichia coli
involved in resistance to antibiotics
?
-
-
?
2.3.1.82
additional information
Moraxella sp.
involved in resistance to antibiotics
?
-
-
?
2.3.1.82
additional information
Escherichia coli CS2R2
involved in resistance to antibiotics
?
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.3.1.82
Escherichia coli
-
CS2R2
-
2.3.1.82
Escherichia coli CS2R2
-
CS2R2
-
2.3.1.82
Moraxella sp.
-
strains 2513 and 731
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.3.1.82
-
Moraxella sp.
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.82
-30°C, stable
Moraxella sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.3.1.82
acetyl-CoA + amikacin
-
636899
Escherichia coli
CoA + N6'-acetylamikacin
-
-
-
?
2.3.1.82
acetyl-CoA + amikacin
-
636899
Moraxella sp.
CoA + N6'-acetylamikacin
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin C1a
-
636899
Escherichia coli
CoA + N6'-acetylgentamicin C1a
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin C1a
-
636899
Moraxella sp.
CoA + N6'-acetylgentamicin C1a
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin A
-
636899
Escherichia coli
CoA + N6'-acetylkanamycin A
-
636899
Escherichia coli
?
2.3.1.82
acetyl-CoA + kanamycin A
-
636899
Moraxella sp.
CoA + N6'-acetylkanamycin A
-
636899
Moraxella sp.
?
2.3.1.82
acetyl-CoA + kanamycin A
-
636899
Escherichia coli CS2R2
CoA + N6'-acetylkanamycin A
-
636899
Escherichia coli CS2R2
?
2.3.1.82
acetyl-CoA + kanamycin B
-
636899
Escherichia coli
CoA + N6'-acetylkanamycin B
-
636899
Escherichia coli
?
2.3.1.82
acetyl-CoA + kanamycin B
-
636899
Moraxella sp.
CoA + N6'-acetylkanamycin B
-
636899
Moraxella sp.
?
2.3.1.82
acetyl-CoA + kanamycin B
-
636899
Escherichia coli CS2R2
CoA + N6'-acetylkanamycin B
-
636899
Escherichia coli CS2R2
?
2.3.1.82
acetyl-CoA + neamine
-
636899
Escherichia coli
CoA + 6'-N-acetylneamine
-
-
-
?
2.3.1.82
acetyl-CoA + neamine
-
636899
Moraxella sp.
CoA + 6'-N-acetylneamine
-
-
-
?
2.3.1.82
acetyl-CoA + neamine
-
636899
Escherichia coli CS2R2
CoA + 6'-N-acetylneamine
-
-
-
?
2.3.1.82
acetyl-CoA + neamine
-
636899
Moraxella sp.
CoA + N6'-acetylneamine
-
-
-
?
2.3.1.82
acetyl-CoA + neomycin C
-
636899
Escherichia coli
CoA + N6'-acetylneomycin C
-
-
-
?
2.3.1.82
acetyl-CoA + neomycin C
-
636899
Moraxella sp.
CoA + N6'-acetylneomycin C
-
-
-
?
2.3.1.82
acetyl-CoA + neomycin C
-
636899
Escherichia coli CS2R2
CoA + N6'-acetylneomycin C
-
-
-
?
2.3.1.82
acetyl-CoA + sisomicin
-
636899
Escherichia coli
CoA + N6'-acetylsisomicin
-
636899
Escherichia coli
?
2.3.1.82
acetyl-CoA + sisomicin
-
636899
Moraxella sp.
CoA + N6'-acetylsisomicin
-
636899
Moraxella sp.
?
2.3.1.82
acetyl-CoA + tobramycin
-
636899
Escherichia coli
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
636899
Moraxella sp.
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
additional information
involved in resistance to antibiotics
636899
Escherichia coli
?
-
-
-
?
2.3.1.82
additional information
involved in resistance to antibiotics
636899
Moraxella sp.
?
-
-
-
?
2.3.1.82
additional information
involved in resistance to antibiotics
636899
Escherichia coli CS2R2
?
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.82
5.3
-
reaction with tobramycin, gentamicin C1a or neomycin
Moraxella sp.
2.3.1.82
6.5
-
reaction with kanamycin, sisomicin, neamine, amikacin
Moraxella sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.82
gentamicin C1
-
Moraxella sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.82
0.004
-
tobramycin
-
Escherichia coli
2.3.1.82
0.004
-
tobramycin
-
Moraxella sp.
2.3.1.82
0.008
-
kanamycin
-
Moraxella sp.
2.3.1.82
0.022
-
tobramycin
-
Escherichia coli
2.3.1.82
0.057
-
amikacin
-
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.3.1.82
additional information
Escherichia coli
involved in resistance to antibiotics
?
-
-
?
2.3.1.82
additional information
Moraxella sp.
involved in resistance to antibiotics
?
-
-
?
2.3.1.82
additional information
Escherichia coli CS2R2
involved in resistance to antibiotics
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.82
-
Moraxella sp.
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.82
-30°C, stable
Moraxella sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.3.1.82
acetyl-CoA + amikacin
-
636899
Escherichia coli
CoA + N6'-acetylamikacin
-
-
-
?
2.3.1.82
acetyl-CoA + amikacin
-
636899
Moraxella sp.
CoA + N6'-acetylamikacin
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin C1a
-
636899
Escherichia coli
CoA + N6'-acetylgentamicin C1a
-
-
-
?
2.3.1.82
acetyl-CoA + gentamicin C1a
-
636899
Moraxella sp.
CoA + N6'-acetylgentamicin C1a
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin A
-
636899
Escherichia coli
CoA + N6'-acetylkanamycin A
-
636899
Escherichia coli
?
2.3.1.82
acetyl-CoA + kanamycin A
-
636899
Moraxella sp.
CoA + N6'-acetylkanamycin A
-
636899
Moraxella sp.
?
2.3.1.82
acetyl-CoA + kanamycin A
-
636899
Escherichia coli CS2R2
CoA + N6'-acetylkanamycin A
-
636899
Escherichia coli CS2R2
?
2.3.1.82
acetyl-CoA + kanamycin B
-
636899
Escherichia coli
CoA + N6'-acetylkanamycin B
-
636899
Escherichia coli
?
2.3.1.82
acetyl-CoA + kanamycin B
-
636899
Moraxella sp.
CoA + N6'-acetylkanamycin B
-
636899
Moraxella sp.
?
2.3.1.82
acetyl-CoA + kanamycin B
-
636899
Escherichia coli CS2R2
CoA + N6'-acetylkanamycin B
-
636899
Escherichia coli CS2R2
?
2.3.1.82
acetyl-CoA + neamine
-
636899
Escherichia coli
CoA + 6'-N-acetylneamine
-
-
-
?
2.3.1.82
acetyl-CoA + neamine
-
636899
Moraxella sp.
CoA + 6'-N-acetylneamine
-
-
-
?
2.3.1.82
acetyl-CoA + neamine
-
636899
Escherichia coli CS2R2
CoA + 6'-N-acetylneamine
-
-
-
?
2.3.1.82
acetyl-CoA + neamine
-
636899
Moraxella sp.
CoA + N6'-acetylneamine
-
-
-
?
2.3.1.82
acetyl-CoA + neomycin C
-
636899
Escherichia coli
CoA + N6'-acetylneomycin C
-
-
-
?
2.3.1.82
acetyl-CoA + neomycin C
-
636899
Moraxella sp.
CoA + N6'-acetylneomycin C
-
-
-
?
2.3.1.82
acetyl-CoA + neomycin C
-
636899
Escherichia coli CS2R2
CoA + N6'-acetylneomycin C
-
-
-
?
2.3.1.82
acetyl-CoA + sisomicin
-
636899
Escherichia coli
CoA + N6'-acetylsisomicin
-
636899
Escherichia coli
?
2.3.1.82
acetyl-CoA + sisomicin
-
636899
Moraxella sp.
CoA + N6'-acetylsisomicin
-
636899
Moraxella sp.
?
2.3.1.82
acetyl-CoA + tobramycin
-
636899
Escherichia coli
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
acetyl-CoA + tobramycin
-
636899
Moraxella sp.
CoA + N6'-acetyltobramycin
-
-
-
?
2.3.1.82
additional information
involved in resistance to antibiotics
636899
Escherichia coli
?
-
-
-
?
2.3.1.82
additional information
involved in resistance to antibiotics
636899
Moraxella sp.
?
-
-
-
?
2.3.1.82
additional information
involved in resistance to antibiotics
636899
Escherichia coli CS2R2
?
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.82
5.3
-
reaction with tobramycin, gentamicin C1a or neomycin
Moraxella sp.
2.3.1.82
6.5
-
reaction with kanamycin, sisomicin, neamine, amikacin
Moraxella sp.