BRENDA - Enzyme Database

Enzymatic acetylation of aminoglycoside antibiotics by Escherichia coli carrying an R factor

Benveniste, R.; Davies, J.; Biochemistry 10, 1787-1796 (1971)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.3.1.82
dialysis in the absence of Mg2+ causes irreversible loss of activity
Escherichia coli
2.3.1.82
Mg2+ stabilizes
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.82
gentamicin A
-
Escherichia coli
2.3.1.82
gentamine A
-
Escherichia coli
2.3.1.82
N6'-Acetylgentamicin C1a
-
Escherichia coli
2.3.1.82
N6'-acetylneomycin B
-
Escherichia coli
2.3.1.82
paromamine
-
Escherichia coli
2.3.1.82
paromomycin
-
Escherichia coli
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.82
Mg2+
required
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.3.1.82
additional information
Escherichia coli
constitutive enzyme
?
-
-
-
2.3.1.82
additional information
Escherichia coli
involved in resistance to antibiotics
?
-
-
-
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.3.1.82
Escherichia coli
-
strains W677 carrying either R factor R-5 or R-79
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.3.1.82
partial
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.82
-20°C, several months
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.3.1.82
acetyl-CoA + gentamicin C1a
-
636898
Escherichia coli
CoA + N6'-acetylgentamicin C1a
-
636898
Escherichia coli
?
2.3.1.82
acetyl-CoA + gentamicin C2
poor substrate, not gentamicin A and C1
636898
Escherichia coli
CoA + N6'-acetylgentgamicin C2
-
-
-
?
2.3.1.82
acetyl-CoA + hybrimycin A1
-
636898
Escherichia coli
CoA + N6'-acetylhybrimycin A1
-
-
-
?
2.3.1.82
acetyl-CoA + hybrimycin A2
-
636898
Escherichia coli
CoA + N6'-acetylhybrimycin A2
-
-
-
?
2.3.1.82
acetyl-CoA + hybrimycin A3
-
636898
Escherichia coli
CoA + N6'-acetylhybrimycin A3
-
-
-
?
2.3.1.82
acetyl-CoA + hybrimycin B1
-
636898
Escherichia coli
CoA + N6'-acetylhybrimycin B1
-
-
-
?
2.3.1.82
acetyl-CoA + hybrimycin B2
-
636898
Escherichia coli
CoA + N6'-acetylhybrimycin B2
-
-
-
?
2.3.1.82
acetyl-CoA + hybrimycin B3
-
636898
Escherichia coli
CoA + N6'-acetylhybrimycin B3
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin A
-
636898
Escherichia coli
CoA + N6'-acetylkanamycin A
-
636898
Escherichia coli
?
2.3.1.82
acetyl-CoA + kanamycin B
-
636898
Escherichia coli
CoA + N6'-acetylkanamycin B
-
636898
Escherichia coli
?
2.3.1.82
acetyl-CoA + neamine
-
636898
Escherichia coli
CoA + 6'-N-acetylneamine
-
-
-
?
2.3.1.82
acetyl-CoA + nebramycin factor 4
no activity with factor 2
636898
Escherichia coli
CoA + N6'-acetylnebramycin factor 4
-
-
-
?
2.3.1.82
acetyl-CoA + nebramycin factor 6
no activity with factor 2
636898
Escherichia coli
CoA + N6'-acetylnebramycin factor 6
-
-
-
?
2.3.1.82
acetyl-CoA + neomycin B
-
636898
Escherichia coli
CoA + N6'-acetylneomycin B
-
636898
Escherichia coli
?
2.3.1.82
acetyl-CoA + neomycin C
-
636898
Escherichia coli
CoA + N6'-acetylneomycin C
-
-
-
?
2.3.1.82
additional information
the smallest antibiotic moiety required for recognition as a substrate by the acetylating enzyme is a 6-amino-6-deoxy-hexose glycosidically linked to a streptamine or deoxystreptamine ring
636898
Escherichia coli
?
-
-
-
-
2.3.1.82
additional information
constitutive enzyme
636898
Escherichia coli
?
-
-
-
-
2.3.1.82
additional information
involved in resistance to antibiotics
636898
Escherichia coli
?
-
-
-
-
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.3.1.82
37
-
pH 7.4, 10 mM MgCl2, 0.6 mM 2-mercaptoethanol, about 35% loss of activity after 10 min, acetyl-CoA prevents, kanamycin protects slightly
Escherichia coli
2.3.1.82
42
-
pH 7.4, 10 mM MgCl2, 0.6 mM 2-mercaptoethanol, about 70% loss of activity after 10 min, acetyl-CoA prevents, kanamycin protects slightly
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.82
5.8
-
reaction with kanamycin, neomycin, hybrimycin, nebramycin factor 4 and 6
Escherichia coli
2.3.1.82
7.6
-
-
Escherichia coli
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.82
5.3
7.6
pH 5.3: about 50% of maximal activity, pH 7.6: about 20% of maximal activity, kanamycin A
Escherichia coli
General Stability (protein specific)
EC Number
General Stability
Organism
2.3.1.82
dialysis in the absence of Mg2+ causes irreversible loss of activity
Escherichia coli
2.3.1.82
Mg2+ stabilizes
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.82
gentamicin A
-
Escherichia coli
2.3.1.82
gentamine A
-
Escherichia coli
2.3.1.82
N6'-Acetylgentamicin C1a
-
Escherichia coli
2.3.1.82
N6'-acetylneomycin B
-
Escherichia coli
2.3.1.82
paromamine
-
Escherichia coli
2.3.1.82
paromomycin
-
Escherichia coli
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.82
Mg2+
required
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.3.1.82
additional information
Escherichia coli
constitutive enzyme
?
-
-
-
2.3.1.82
additional information
Escherichia coli
involved in resistance to antibiotics
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.82
partial
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.82
-20°C, several months
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.3.1.82
acetyl-CoA + gentamicin C1a
-
636898
Escherichia coli
CoA + N6'-acetylgentamicin C1a
-
636898
Escherichia coli
?
2.3.1.82
acetyl-CoA + gentamicin C2
poor substrate, not gentamicin A and C1
636898
Escherichia coli
CoA + N6'-acetylgentgamicin C2
-
-
-
?
2.3.1.82
acetyl-CoA + hybrimycin A1
-
636898
Escherichia coli
CoA + N6'-acetylhybrimycin A1
-
-
-
?
2.3.1.82
acetyl-CoA + hybrimycin A2
-
636898
Escherichia coli
CoA + N6'-acetylhybrimycin A2
-
-
-
?
2.3.1.82
acetyl-CoA + hybrimycin A3
-
636898
Escherichia coli
CoA + N6'-acetylhybrimycin A3
-
-
-
?
2.3.1.82
acetyl-CoA + hybrimycin B1
-
636898
Escherichia coli
CoA + N6'-acetylhybrimycin B1
-
-
-
?
2.3.1.82
acetyl-CoA + hybrimycin B2
-
636898
Escherichia coli
CoA + N6'-acetylhybrimycin B2
-
-
-
?
2.3.1.82
acetyl-CoA + hybrimycin B3
-
636898
Escherichia coli
CoA + N6'-acetylhybrimycin B3
-
-
-
?
2.3.1.82
acetyl-CoA + kanamycin A
-
636898
Escherichia coli
CoA + N6'-acetylkanamycin A
-
636898
Escherichia coli
?
2.3.1.82
acetyl-CoA + kanamycin B
-
636898
Escherichia coli
CoA + N6'-acetylkanamycin B
-
636898
Escherichia coli
?
2.3.1.82
acetyl-CoA + neamine
-
636898
Escherichia coli
CoA + 6'-N-acetylneamine
-
-
-
?
2.3.1.82
acetyl-CoA + nebramycin factor 4
no activity with factor 2
636898
Escherichia coli
CoA + N6'-acetylnebramycin factor 4
-
-
-
?
2.3.1.82
acetyl-CoA + nebramycin factor 6
no activity with factor 2
636898
Escherichia coli
CoA + N6'-acetylnebramycin factor 6
-
-
-
?
2.3.1.82
acetyl-CoA + neomycin B
-
636898
Escherichia coli
CoA + N6'-acetylneomycin B
-
636898
Escherichia coli
?
2.3.1.82
acetyl-CoA + neomycin C
-
636898
Escherichia coli
CoA + N6'-acetylneomycin C
-
-
-
?
2.3.1.82
additional information
the smallest antibiotic moiety required for recognition as a substrate by the acetylating enzyme is a 6-amino-6-deoxy-hexose glycosidically linked to a streptamine or deoxystreptamine ring
636898
Escherichia coli
?
-
-
-
-
2.3.1.82
additional information
constitutive enzyme
636898
Escherichia coli
?
-
-
-
-
2.3.1.82
additional information
involved in resistance to antibiotics
636898
Escherichia coli
?
-
-
-
-
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.3.1.82
37
-
pH 7.4, 10 mM MgCl2, 0.6 mM 2-mercaptoethanol, about 35% loss of activity after 10 min, acetyl-CoA prevents, kanamycin protects slightly
Escherichia coli
2.3.1.82
42
-
pH 7.4, 10 mM MgCl2, 0.6 mM 2-mercaptoethanol, about 70% loss of activity after 10 min, acetyl-CoA prevents, kanamycin protects slightly
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.82
5.8
-
reaction with kanamycin, neomycin, hybrimycin, nebramycin factor 4 and 6
Escherichia coli
2.3.1.82
7.6
-
-
Escherichia coli
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.82
5.3
7.6
pH 5.3: about 50% of maximal activity, pH 7.6: about 20% of maximal activity, kanamycin A
Escherichia coli