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Literature summary extracted from

  • Kaushal, G.P.; Elbein, A.D.
    Partial purification and characterization of beta-mannosyltransferase from suspension-cultured soybean cells (1987), Biochemistry, 26, 7953-7960.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.142 NP-40
-
 Glycine max
2.4.1.142 phosphatidic acid
-
 Glycine max
2.4.1.142 phosphatidylcholine
-
 Glycine max
2.4.1.142 phosphatidylserine
-
 Glycine max
2.4.1.142 Phospholipid
-
 Glycine max
2.4.1.142 Triton X-100 optimum activity at 0.08-0.1% Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.142 GDP
-
 Glycine max
2.4.1.142 GDP-glucose
-
 Glycine max
2.4.1.142 GMP
-
 Glycine max
2.4.1.142 GTP
-
 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.142 0.0017
-
 GDP-mannose
-
 Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.142 microsome
-
 Glycine max
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.142 Mg2+ requirement for activity Glycine max

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.142 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.142 DEAE-cellulose, GDP-affinity column Glycine max

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.142 additional information
-
-
 Glycine max

Storage Stability

EC Number Storage Stability Organism
2.4.1.142 frozen, Tris-buffer, pH 7.0, 0.5 mM DTT, 20% glycerol, 0.1% detergent, 1 month Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.142 GDP-alpha-D-mannose + chitobiosyldiphosphodolichol
-
 Glycine max GDP + beta-(1->4)-D-mannosylchitobiosyldiphosphodolichol
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.142 6.9 7
-
 Glycine max