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Literature summary extracted from
- Biochemical studies on pollen. Part XXXIV. UDP-glucose:4-(beta-D-glucopyranosyloxy)benzoic acid glucosyltransferase from the pollen of Pinus densiflora (1989), Phytochemistry, 28, 359-362.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.194 | EDTA | 60% increase of activity at 0.1 mM, probably due to removal of heavy metal ions | Pinus densiflora |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.194 | 2-hydroxybenzoic acid | - |
Pinus densiflora | |
| 2.4.1.194 | 3-Hydroxybenzoic acid | competitive inhibition | Pinus densiflora | |
| 2.4.1.194 | 4-coumaric acid | - |
Pinus densiflora | |
| 2.4.1.194 | 4-hydroxybenzoic acid | - |
Pinus densiflora | |
| 2.4.1.194 | gallic acid | competitive inhibition | Pinus densiflora | |
| 2.4.1.194 | vanillic acid | competitive inhibition | Pinus densiflora | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.194 | 0.24 | - |
UDPglucose | - |
Pinus densiflora | |
| 2.4.1.194 | 2.9 | - |
4-hydroxybenzoate | - |
Pinus densiflora | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.194 | Ca2+ | 50% increase of activity at 0.3 mM | Pinus densiflora | |
| 2.4.1.194 | additional information | Mg2+ has no effect | Pinus densiflora | |
| 2.4.1.194 | additional information | no metal ions required | Pinus densiflora |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.194 | 33000 | - |
apparent, from gel filtration | Pinus densiflora |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.194 | additional information | Pinus densiflora | probably control of activity during pine pollen germination | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.194 | Pinus densiflora | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.194 | partial | Pinus densiflora |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.4.1.194 | pollen | - |
Pinus densiflora | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.194 | additional information | probably control of activity during pine pollen germination | Pinus densiflora | ? | - |
? | |
| 2.4.1.194 | UDPglucose + 4-hydroxybenzoate | - |
Pinus densiflora | UDP + 4-(beta-D-glucosyloxy)benzoate | - |
ir | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.194 | 30 | - |
assay at | Pinus densiflora |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.194 | 7.5 | - |
- |
Pinus densiflora |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.194 | 0.4 | - |
3-Hydroxybenzoic acid | - |
Pinus densiflora | |
| 2.4.1.194 | 0.7 | - |
gallic acid | - |
Pinus densiflora | |
| 2.4.1.194 | 1.3 | - |
vanillic acid | - |
Pinus densiflora | |
| 2.4.1.194 | 3.1 | - |
2-hydroxybenzoic acid | - |
Pinus densiflora | |
| 2.4.1.194 | 5 | - |
4-coumaric acid | - |
Pinus densiflora | |
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