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Literature summary extracted from

  • McCleary, B.V.; Chick, B.F.
    The purification and properties of a thiaminase I enzyme from nardoo (Marsilea Drumondii) (1977), Phytochemistry, 16, 207-213.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.2 2-mercaptoethanol complete reactivation at 3.3 mM but inhibition at 33 mM Marsilea drummondii
2.5.1.2 cysteine complete reactivation at 3.3 mM but inhibition at 33 mM Marsilea drummondii
2.5.1.2 Thioglycollate greater reactivation at 33 mM than at 3.3 mM Marsilea drummondii
2.5.1.2 thiosulphate greater reactivation at 33 mM than at 3.3 mM Marsilea drummondii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.2 Ag+ 100% inhibition at 1 mM Marsilea drummondii
2.5.1.2 Cu2+ 50% of inhibition at 1 mM Marsilea drummondii
2.5.1.2 Fe2+ 70% of inhibition at 1 mM Marsilea drummondii
2.5.1.2 HgCl2 potent inhibitor at 0.001 mM Cheilanthes sieberi
2.5.1.2 HgCl2 potent inhibitor at 0.001 mM Marsilea drummondii
2.5.1.2 HgCl2 potent inhibitor at 0.001 mM Pteridium esculentum
2.5.1.2 HgCl2 potent inhibitor at 0.001 mM Velesunio ambiguus
2.5.1.2 Mersalyl acid potent inhibitor at 0.001 mM Cheilanthes sieberi
2.5.1.2 Mersalyl acid potent inhibitor at 0.001 mM Marsilea drummondii
2.5.1.2 Mersalyl acid potent inhibitor at 0.001 mM Pteridium esculentum
2.5.1.2 Mersalyl acid potent inhibitor at 0.001 mM Velesunio ambiguus
2.5.1.2 Mn2+ no inhibition Marsilea drummondii
2.5.1.2 additional information not inhibited by EDTA Marsilea drummondii
2.5.1.2 p-chloromercuribenzoate complete inhibition at 0.001 mM Cheilanthes sieberi
2.5.1.2 p-chloromercuribenzoate complete inhibition at 0.001 mM Marsilea drummondii
2.5.1.2 p-chloromercuribenzoate complete inhibition at 0.001 mM Pteridium esculentum
2.5.1.2 p-chloromercuribenzoate complete inhibition at 0.001 mM Velesunio ambiguus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.2 0.003
-
 thiamine measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate Marsilea drummondii
2.5.1.2 0.003
-
 thiamine measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate Pteridium esculentum
2.5.1.2 0.003
-
 thiamine measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate Cheilanthes sieberi
2.5.1.2 0.003
-
 thiamine measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate Velesunio ambiguus
2.5.1.2 0.5
-
 beta-picoline
-
 Marsilea drummondii
2.5.1.2 0.7
-
 Pyridine
-
 Pteridium esculentum
2.5.1.2 0.7
-
 Pyridine
-
 Velesunio ambiguus
2.5.1.2 0.9
-
 nicotinic acid
-
 Velesunio ambiguus
2.5.1.2 0.9
-
 gamma-Picoline
-
 Marsilea drummondii
2.5.1.2 1.4
-
 aniline lower maximal activity than pyridine and nicotinic acid Marsilea drummondii
2.5.1.2 1.4
-
 aniline lower maximal activity than pyridine and nicotinic acid Pteridium esculentum
2.5.1.2 1.7
-
 m-phenylene diamine
-
 Marsilea drummondii
2.5.1.2 1.7
-
 o-phenylene diamine
-
 Marsilea drummondii
2.5.1.2 2
-
 aniline lower maximal activity than pyridine and nicotinic acid Velesunio ambiguus
2.5.1.2 2.4
-
 m-Toluidine
-
 Marsilea drummondii
2.5.1.2 2.5
-
 Pyridine
-
 Marsilea drummondii
2.5.1.2 2.8
-
 3-hydroxyaniline
-
 Marsilea drummondii
2.5.1.2 2.9
-
 3-aminopyridine
-
 Marsilea drummondii
2.5.1.2 3.3
-
 Quinoline
-
 Marsilea drummondii
2.5.1.2 3.3
-
 alpha-picoline
-
 Marsilea drummondii
2.5.1.2 3.3
-
 p-phenylene diamine
-
 Marsilea drummondii
2.5.1.2 3.4
-
 gamma-picolinic acid
-
 Marsilea drummondii
2.5.1.2 4.2
-
 2-hydroxyaniline
-
 Marsilea drummondii
2.5.1.2 5
-
 Pyridine
-
 Cheilanthes sieberi
2.5.1.2 5
-
 o-mercaptobenzoic acid best cosubstrate Marsilea drummondii
2.5.1.2 5.1
-
 aniline lower maximal activity than pyridine and nicotinic acid Cheilanthes sieberi
2.5.1.2 6.4
-
 o-toluidine
-
 Marsilea drummondii
2.5.1.2 9.5
-
 adenine
-
 Marsilea drummondii
2.5.1.2 12.5
-
 2-aminopyridine
-
 Marsilea drummondii
2.5.1.2 15.4
-
 4-aminopyridine
-
 Marsilea drummondii
2.5.1.2 20
-
 4-Hydroxyaniline
-
 Marsilea drummondii
2.5.1.2 21.2
-
 alpha-picolinic acid
-
 Marsilea drummondii
2.5.1.2 25
-
 nicotinic acid
-
 Marsilea drummondii
2.5.1.2 25
-
 beta-picolinic acid
-
 Marsilea drummondii
2.5.1.2 29
-
 nicotinic acid
-
 Pteridium esculentum
2.5.1.2 32
-
 methylamine
-
 Marsilea drummondii
2.5.1.2 33
-
 L-cysteine
-
 Marsilea drummondii
2.5.1.2 37
-
 o-aminobenzoic acid
-
 Marsilea drummondii
2.5.1.2 37
-
 m-aminobenzoic acid
-
 Marsilea drummondii
2.5.1.2 38
-
 6-aminohexanoic acid
-
 Marsilea drummondii
2.5.1.2 50
-
 Sulfanilic acid
-
 Marsilea drummondii
2.5.1.2 50
-
 3-nitroaniline
-
 Marsilea drummondii
2.5.1.2 55
-
 nicotinic acid
-
 Cheilanthes sieberi
2.5.1.2 77
-
 sulfanilamide
-
 Marsilea drummondii
2.5.1.2 111
-
 proline
-
 Marsilea drummondii
2.5.1.2 125
-
 imidazole
-
 Marsilea drummondii
2.5.1.2 200
-
 Tris
-
 Marsilea drummondii
2.5.1.2 200
-
 p-Aminobenzoic acid
-
 Marsilea drummondii
2.5.1.2 400
-
 hydroxyproline
-
 Marsilea drummondii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.2 additional information Ca2+, Mo+, Mn2+, Zn2+, Mg2+ have no effect on activity Marsilea drummondii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.2 93000
-
 gel filtration Pteridium esculentum
2.5.1.2 107000
-
 gel filtration Cheilanthes sieberi
2.5.1.2 110000
-
 gel filtration Velesunio ambiguus
2.5.1.2 115000
-
 gel filtration Marsilea drummondii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.2 thiamine + adenine Marsilea drummondii
-
 ?
-
 ?
2.5.1.2 thiamine + hydroxyproline Marsilea drummondii
-
 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid
-
 ?
2.5.1.2 thiamine + hydroxyproline Pteridium esculentum
-
 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid
-
 ?
2.5.1.2 thiamine + proline Marsilea drummondii
-
 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid
-
 ?
2.5.1.2 thiamine + proline Pteridium esculentum
-
 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.2 Cheilanthes sieberi
-
 rock fern
-
2.5.1.2 Marsilea angustifolia
-
-
-
2.5.1.2 Marsilea drummondii
-
 nardoo
-
2.5.1.2 Marsilea mutica
-
-
-
2.5.1.2 Pteridium esculentum
-
 bracken fern
-
2.5.1.2 Velesunio ambiguus
-
 freshwater mussel
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.2 by DEAE-cellulose chromatrography, ultrogel AcA 34 column, Sephadex G-100 column and phosphocellulose column Marsilea drummondii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.2 frond extremely high levels of the enzyme, lush fronds have much more enzyme than old fronds Marsilea drummondii
-
2.5.1.2 frond activity found in lush fronds Marsilea mutica
-
2.5.1.2 frond activity found in lush fronds Cheilanthes sieberi
-
2.5.1.2 frond very low activity in lush fronds Marsilea angustifolia
-
2.5.1.2 frond very low activity in youngs and old fronds Pteridium esculentum
-
2.5.1.2 root lush growth Marsilea drummondii
-
2.5.1.2 tuber very low activity Pteridium esculentum
-
2.5.1.2 whole body activity found Velesunio ambiguus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.2 thiamine + 2-aminopyridine
-
 Marsilea drummondii ?
-
 ?
2.5.1.2 thiamine + 2-hydroxyaniline
-
 Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-hydroxyphenyl)amine
-
 ?
2.5.1.2 thiamine + 2-mercaptobenzoic acid
-
 Marsilea drummondii ?
-
 ?
2.5.1.2 thiamine + 2-methylbenzeneamine i.e. o-toluidine Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-methylphenyl)amine
-
 ?
2.5.1.2 thiamine + 2-pyridinecarboxylic acid i.e. alpha-picolinic acid Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxypyridinium
-
 ?
2.5.1.2 thiamine + 3-aminopyridine
-
 Marsilea drummondii ?
-
 ?
2.5.1.2 thiamine + 3-hydroxyaniline
-
 Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-hydroxyphenyl)amine
-
 ?
2.5.1.2 thiamine + 3-methylbenzeneamine i.e. m-toluidine Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-methylphenyl)amine
-
 ?
2.5.1.2 thiamine + 3-nitroaniline
-
 Marsilea drummondii ?
-
 ?
2.5.1.2 thiamine + 3-pyridinecarboxylic acid i.e. beta-picolinic acid Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-3-carboxypyridinium
-
 ?
2.5.1.2 thiamine + 4-aminopyridine
-
 Marsilea drummondii ?
-
 ?
2.5.1.2 thiamine + 4-hydroxyaniline
-
 Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-hydroxyphenyl)amine
-
 ?
2.5.1.2 thiamine + 4-methylbenzeneamine i.e. p-toluidine Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-methylphenyl)amine
-
 ?
2.5.1.2 thiamine + 4-pyridinecarboxylic acid i.e. gamma-picolinic acid Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-carboxypyridinium
-
 ?
2.5.1.2 thiamine + 6-aminohexanoic acid
-
 Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + 6-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]hexanoic acid
-
 ?
2.5.1.2 thiamine + adenine
-
 Marsilea drummondii ?
-
 ?
2.5.1.2 thiamine + alpha-picoline
-
 Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]alpha-picolinium
-
 ?
2.5.1.2 thiamine + aniline
-
 Marsilea drummondii 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
 ?
2.5.1.2 thiamine + aniline
-
 Pteridium esculentum 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
 ?
2.5.1.2 thiamine + aniline
-
 Cheilanthes sieberi 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
 ?
2.5.1.2 thiamine + aniline
-
 Velesunio ambiguus 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
 ?
2.5.1.2 thiamine + beta-picoline
-
 Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]beta-picolinium
-
 ?
2.5.1.2 thiamine + gamma-picoline
-
 Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]gamma-picolinium
-
 ?
2.5.1.2 thiamine + hydroxyproline
-
 Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid
-
 ?
2.5.1.2 thiamine + hydroxyproline
-
 Pteridium esculentum 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid
-
 ?
2.5.1.2 thiamine + imidazole
-
 Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + 5-(1H-imidazol-1-yl-methyl)-2-methylpyrimidin-4-amine
-
 ?
2.5.1.2 thiamine + L-cysteine
-
 Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-ethylpyrimidin-5-yl)methyl]amino]-3-mercaptopropanoic acid
-
 ?
2.5.1.2 thiamine + m-aminobenzoic acid
-
 Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + 3-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid
-
 ?
2.5.1.2 thiamine + m-phenylene diamine
-
 Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-aminophenyl)amine
-
 ?
2.5.1.2 thiamine + methylamine
-
 Marsilea drummondii ?
-
 ?
2.5.1.2 thiamine + nicotinic acid
-
 Pteridium esculentum ?
-
 ?
2.5.1.2 thiamine + nicotinic acid
-
 Cheilanthes sieberi ?
-
 ?
2.5.1.2 thiamine + nicotinic acid
-
 Velesunio ambiguus ?
-
 ?
2.5.1.2 thiamine + o-aminobenzoic acid
-
 Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid
-
 ?
2.5.1.2 thiamine + o-phenylene diamine
-
 Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-aminophenyl)amine
-
 ?
2.5.1.2 thiamine + p-aminobenzoic acid
-
 Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + 4-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid
-
 ?
2.5.1.2 thiamine + p-phenylene diamine
-
 Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-aminophenyl)amine
-
 ?
2.5.1.2 thiamine + proline
-
 Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid
-
 ?
2.5.1.2 thiamine + proline
-
 Pteridium esculentum 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid
-
 ?
2.5.1.2 thiamine + pyridine
-
 Marsilea drummondii heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
 ?
2.5.1.2 thiamine + pyridine
-
 Pteridium esculentum heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
 ?
2.5.1.2 thiamine + pyridine
-
 Cheilanthes sieberi heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
 ?
2.5.1.2 thiamine + pyridine
-
 Velesunio ambiguus heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
 ?
2.5.1.2 thiamine + quinoline
-
 Marsilea drummondii 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]quinolinium
-
 ?
2.5.1.2 thiamine + sulfanilamide
-
 Marsilea drummondii ?
-
 ?
2.5.1.2 thiamine + sulfanilic acid
-
 Marsilea drummondii ?
-
 ?
2.5.1.2 thiamine + Tris
-
 Marsilea drummondii ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.2 8 9
-
 Marsilea drummondii
2.5.1.2 8 9
-
 Pteridium esculentum
2.5.1.2 8 9
-
 Cheilanthes sieberi
2.5.1.2 8 9
-
 Velesunio ambiguus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.2 3 12
-
 Marsilea drummondii
2.5.1.2 3 12
-
 Pteridium esculentum
2.5.1.2 3 12
-
 Cheilanthes sieberi
2.5.1.2 3 12
-
 Velesunio ambiguus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.2 3 12 stable in this range Marsilea drummondii
2.5.1.2 3 12 stable in this range Pteridium esculentum
2.5.1.2 3 12 stable in this range Cheilanthes sieberi
2.5.1.2 3 12 stable in this range Velesunio ambiguus