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Literature summary extracted from

  • Munch-Petersen, B.; Knecht, W.; Lenz, C.; Sondergaard, L.; Piskur, J.
    Functional expression of a multisubstrate deoxyribonucleoside kinase from Drosophila melanogaster and its C-terminal deletion mutants (2000), J. Biol. Chem., 275, 6673-6679.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.145 expression in Escherichia coli Drosophila melanogaster
2.7.1.145 wild-type and truncated enzyme forms with removal of 10, 20 or 30 C-terminal amino acids, expression as a fusion protein with glutathione S-transferase Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.145 additional information the deletion mutants rDm-dNKDELTAC10 and rDm-dNKDELTAC20 show the same substrate activity pattern as the recombinant wild-type enzyme. Relative phosphorylation of 2'-deoxycytidine and 2-chloro-2'-deoxyadenosine increases with increasing C-terminal truncation. The relative activities of rDm-dNKDELTAC10 and rDm-dNKDELTAC20 with deoxyribonucleosides remains largely unchanged, whereas there is a substantial decrease in the phosphorylation of the purine ribonucleosides adenosine and guanosine, as well as of all dideoxyribonucleosides and 3'-azido-2',3'-dideoxythymidine. The relative activities with the pyrimidine ribonucleosides and 1-beta-D-arabinofuranosylcytosine and 1-beta-D-arabinofuranosylthymine are not affected by the C-terminal deletions Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.145 0.0012
-
thymidine pH 7.8, 22°C, recombinant enzyme Drosophila melanogaster
2.7.1.145 0.0023
-
2'-deoxycytidine pH 7.8, 22°C, recombinant enzyme Drosophila melanogaster
2.7.1.145 0.225
-
2'-deoxyadenosine pH 7.8, 22°C, recombinant enzyme Drosophila melanogaster
2.7.1.145 0.665
-
2'-deoxyguanosine pH 7.8, 22°C, recombinant enzyme Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.145 29000
-
1 * 29000, SDS-PAGE Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.145 Drosophila melanogaster Q9XZT6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.145 recombinant enzyme Drosophila melanogaster

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.145 0.35
-
C-terminal truncated enzyme rDm-dNKDELTAC30 Drosophila melanogaster
2.7.1.145 23.8
-
C-terminal truncated enzyme rDm-dNKDELTAC10 Drosophila melanogaster
2.7.1.145 36.5
-
recombinant enzyme Drosophila melanogaster
2.7.1.145 51.6
-
C-terminal truncated enzyme rDm-dNKDELTAC20 Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.145 (E)-5-(2-bromovinyl)-2'-deoxyuridine + ATP 54% of the activity with thymidine Drosophila melanogaster (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate + ADP
-
?
2.7.1.145 1-beta-D-arabinofuranosylcytosine + ATP 58.2% of the activity with thymidine Drosophila melanogaster 1-beta-D-arabinofuranosylcytosine 5'-phosphate + ADP
-
?
2.7.1.145 1-beta-D-arabinofuranosylthymine + ATP 53.9% of the activity with thymidine Drosophila melanogaster 1-beta-D-arabinofuranosylthymine 5'-phosphate + ADP
-
?
2.7.1.145 2',3'-dideoxyadenosine + ATP 0.9% of the activity with thymidine Drosophila melanogaster 2',3'-dideoxyadenosine 5'-phosphate + ADP
-
?
2.7.1.145 2',3'-dideoxycytidine + ATP 11.1% of the activity with thymidine Drosophila melanogaster 2',3'-dideoxycytidine 5'-phosphate + ADP
-
?
2.7.1.145 2',3'-dideoxythymidine + ATP 4.44% of the activity with thymidine Drosophila melanogaster 2',3'-dideoxythymidine 5'-phosphate + ADP
-
?
2.7.1.145 2'-deoxyadenosine + ATP 96% of the activity with thymidine Drosophila melanogaster 2'-deoxyadenosine 5'-phosphate + ADP
-
?
2.7.1.145 2'-deoxycytidine + ATP 134% of the activity with thymidine Drosophila melanogaster 2'-deoxycytidine 5'-phosphate + ADP
-
?
2.7.1.145 2'-deoxyguanosine + ATP 53% of the activity with thymidine Drosophila melanogaster 2'-deoxyguanosine 5'-phosphate + ADP
-
?
2.7.1.145 2'-deoxyuridine + ATP 113% of the activity with thymidine Drosophila melanogaster 2'-deoxyuridine 5'-phosphate + ADP
-
?
2.7.1.145 2-chloro-2'-deoxyadenosine + ATP 126% of the activity with thymidine Drosophila melanogaster 2-chloro-2'-deoxyadenosine 5'-phosphate + ADP
-
?
2.7.1.145 3'-azido-2',3'-dideoxythymidine + ATP 2% of the activity with thymidine Drosophila melanogaster 3'-azido-2',3'-dideoxythymidine 5'-phosphate + ADP
-
?
2.7.1.145 5-fluoro-2'-deoxyuridine + ATP 92% of the activity with thymidine Drosophila melanogaster 5-fluoro-2'-deoxyuridine 5'-phosphate + ADP
-
?
2.7.1.145 adenosine + ATP 0.82% of the activity with thymidine Drosophila melanogaster adenosine 5'-phosphate + ADP
-
?
2.7.1.145 cytidine + ATP 16% of the activity with thymidine Drosophila melanogaster cytidine 5'-phosphate + ADP
-
?
2.7.1.145 guanosine + ATP 1.18% of the activity with thymidine Drosophila melanogaster guanosine 5'-phosphate + ADP
-
?
2.7.1.145 thymidine + ATP
-
Drosophila melanogaster thymidine 5'-phosphate + ADP
-
?
2.7.1.145 uridine + ATP 6.45% of the activity with thymidine Drosophila melanogaster uridine 5'-phosphate + ADP
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.145 monomer 1 * 29000, SDS-PAGE Drosophila melanogaster

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.145 14.2
-
thymidine pH 7.8, 22°C, recombinant enzyme Drosophila melanogaster
2.7.1.145 15.1
-
2'-deoxyguanosine pH 7.8, 22°C, recombinant enzyme Drosophila melanogaster
2.7.1.145 16.5
-
2'-deoxycytidine pH 7.8, 22°C, recombinant enzyme Drosophila melanogaster
2.7.1.145 20.6
-
2'-deoxyadenosine pH 7.8, 22°C, recombinant enzyme Drosophila melanogaster