BRENDA - Enzyme Database

Four deoxynucleoside kinase activities from Drosophila melanogaster are contained within a single monomeric enzyme, a new multifunctional deoxynucleoside kinase

Munch-Petersen, B.; Piskur, J.; Sondergaard, L.; J. Biol. Chem. 273, 3926-3931 (1998)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.145
2'-deoxyadenosine
reaction with thymidine or 2'-deoxyguanosine
Drosophila melanogaster
2.7.1.145
2'-deoxycytidine
competitive with thymidine
Drosophila melanogaster
2.7.1.145
2'-deoxyguanosine
reaction with thymidine or 2'-deoxyadenosine
Drosophila melanogaster
2.7.1.145
thymidine
strong inhibitor of reaction with 2'-deoxycytidine, 2'-deoxyguanosine or 2'-deoxyadenosine
Drosophila melanogaster
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.145
0.0009
-
thymidine
pH 8.0, 22°C
Drosophila melanogaster
2.7.1.145
0.001
-
2'-deoxycytidine
pH 8.0, 22°C
Drosophila melanogaster
2.7.1.145
0.028
-
1-beta-D-arabinofuranosylcytosine
-
Drosophila melanogaster
2.7.1.145
0.109
-
2'-deoxyadenosine
pH 8.0, 22°C
Drosophila melanogaster
2.7.1.145
0.654
-
2'-deoxyguanosine
pH 8.0, 22°C
Drosophila melanogaster
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.145
30000
-
1 * 30000, SDS-PAGE
Drosophila melanogaster
2.7.1.145
33000
-
gel filtration
Drosophila melanogaster
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.145
Drosophila melanogaster
-
S-2
-
2.7.1.145
Drosophila melanogaster S-2
-
S-2
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.145
-
Drosophila melanogaster
Reaction
EC Number
Reaction
Commentary
Organism
2.7.1.145
ATP + a 2'-deoxyribonucleoside = ADP + a 2'-deoxyribonucleoside 5'-phosphate
compulsory ordered steady-state reaction mechanism with formation of a ternary complex with the phosphate donor and acceptor
Drosophila melanogaster
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.145
embryo
-
Drosophila melanogaster
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.145
29.4
-
-
Drosophila melanogaster
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.145
1-beta-D-arabinofuranosylcytosine + ATP
10% of the activity with thymidine and 2'-deoxycytidine
636840
Drosophila melanogaster
1-beta-D-arabinofuranosylcytosine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
1-beta-D-arabinofuranosylthymine + ATP
15% of the activity with thymidine and 2'-deoxycytidine
636840
Drosophila melanogaster
1-beta-D-arabinofuranosylthymine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2',3'-dideoxycytidine + ATP
0.02% of the activity with thymidine and 2'-deoxycytidine
636840
Drosophila melanogaster
2',3'-dideoxycytidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxyadenosine + ATP
5% of the activity with thymidine and 2'-deoxycytidine
636840
Drosophila melanogaster
2'-deoxyadenosine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxycytidine + ATP
-
636840
Drosophila melanogaster
2'-deoxycytidine 5'-phosphate + ADP
-
636840
Drosophila melanogaster
?
2.7.1.145
2'-deoxycytidine + ATP
-
636840
Drosophila melanogaster S-2
2'-deoxycytidine 5'-phosphate + ADP
-
636840
Drosophila melanogaster S-2
?
2.7.1.145
2'-deoxycytidine + CTP
-
636840
Drosophila melanogaster
2'-deoxycytidine 5'-phosphate + CDP
-
-
-
?
2.7.1.145
2'-deoxyguanosine + ATP
1% of the activity with thymidine and 2'-deoxycytidine
636840
Drosophila melanogaster
2'-deoxyguanosine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxyuridine + ATP
90% of the activity with thymidine and 2'-deoxycytidine
636840
Drosophila melanogaster
2'-deoxyuridine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
3'-azidothymidine + ATP
0.5% of the activity with thymidine and 2'-deoxycytidine
636840
Drosophila melanogaster
3'-azidothymidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
3'-fluoro-2',3'-dideoxythymidine + ATP
0.03% of the activity with thymidine and 2'-deoxycytidine
636840
Drosophila melanogaster
3'-fluoro-2',3'-dideoxythymidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
5-fluoro-2'-deoxyuridine + ATP
activity is 10% higher than with thymidine and 2'-deoxycytidine
636840
Drosophila melanogaster
5-fluoro-2'-deoxyuridine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
additional information
no activity with acyclovir
636840
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
additional information
activity with CTP is 110% of the activity ATP
636840
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
additional information
no activity with dTMP
636840
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
additional information
no activity with acyclovir
636840
Drosophila melanogaster S-2
?
-
-
-
-
2.7.1.145
additional information
activity with CTP is 110% of the activity ATP
636840
Drosophila melanogaster S-2
?
-
-
-
-
2.7.1.145
additional information
no activity with dTMP
636840
Drosophila melanogaster S-2
?
-
-
-
-
2.7.1.145
thymidine + ATP
-
636840
Drosophila melanogaster
thymidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
thymidine + ATP
-
636840
Drosophila melanogaster S-2
thymidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
thymidine + CTP
-
636840
Drosophila melanogaster
thymidine 5'-phosphate + CDP
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.145
monomer
1 * 30000, SDS-PAGE
Drosophila melanogaster
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.145
40
60
-
Drosophila melanogaster
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.145
15
-
2'-deoxycytidine
pH 8.0, 22°C
Drosophila melanogaster
2.7.1.145
15
-
thymidine
pH 8.0, 22°C
Drosophila melanogaster
2.7.1.145
18
19
2'-deoxyadenosine
pH 8.0, 22°C
Drosophila melanogaster
2.7.1.145
18
19
2'-deoxyguanosine
pH 8.0, 22°C
Drosophila melanogaster
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.145
0.001
-
2'-deoxycytidine
pH 8.0, 22°C, competitive with thymidine
Drosophila melanogaster
2.7.1.145
0.001
-
thymidine
pH 8.0, 22°C, competitive with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
0.002
-
thymidine
pH 8.0, 22°C, reaction with 2'-deoxyadenosine
Drosophila melanogaster
2.7.1.145
0.003
-
thymidine
pH 8.0, 22°C, reaction with 2'-deoxyguanosine
Drosophila melanogaster
2.7.1.145
0.02
-
2'-deoxyadenosine
pH 8.0, 22°C, reaction with thymidine
Drosophila melanogaster
2.7.1.145
0.1
-
2'-deoxyguanosine
pH 8.0, 22°C, reaction with thymidine
Drosophila melanogaster
2.7.1.145
0.16
-
2'-deoxyadenosine
pH 8.0, 22°C, reaction with 2'-deoxyguanosine
Drosophila melanogaster
2.7.1.145
0.4
-
2'-deoxyguanosine
pH 8.0, 22°C, reaction with 2'-deoxyadenosine
Drosophila melanogaster
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.145
2'-deoxyadenosine
reaction with thymidine or 2'-deoxyguanosine
Drosophila melanogaster
2.7.1.145
2'-deoxycytidine
competitive with thymidine
Drosophila melanogaster
2.7.1.145
2'-deoxyguanosine
reaction with thymidine or 2'-deoxyadenosine
Drosophila melanogaster
2.7.1.145
thymidine
strong inhibitor of reaction with 2'-deoxycytidine, 2'-deoxyguanosine or 2'-deoxyadenosine
Drosophila melanogaster
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.145
0.001
-
2'-deoxycytidine
pH 8.0, 22°C, competitive with thymidine
Drosophila melanogaster
2.7.1.145
0.001
-
thymidine
pH 8.0, 22°C, competitive with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
0.002
-
thymidine
pH 8.0, 22°C, reaction with 2'-deoxyadenosine
Drosophila melanogaster
2.7.1.145
0.003
-
thymidine
pH 8.0, 22°C, reaction with 2'-deoxyguanosine
Drosophila melanogaster
2.7.1.145
0.02
-
2'-deoxyadenosine
pH 8.0, 22°C, reaction with thymidine
Drosophila melanogaster
2.7.1.145
0.1
-
2'-deoxyguanosine
pH 8.0, 22°C, reaction with thymidine
Drosophila melanogaster
2.7.1.145
0.16
-
2'-deoxyadenosine
pH 8.0, 22°C, reaction with 2'-deoxyguanosine
Drosophila melanogaster
2.7.1.145
0.4
-
2'-deoxyguanosine
pH 8.0, 22°C, reaction with 2'-deoxyadenosine
Drosophila melanogaster
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.145
0.0009
-
thymidine
pH 8.0, 22°C
Drosophila melanogaster
2.7.1.145
0.001
-
2'-deoxycytidine
pH 8.0, 22°C
Drosophila melanogaster
2.7.1.145
0.028
-
1-beta-D-arabinofuranosylcytosine
-
Drosophila melanogaster
2.7.1.145
0.109
-
2'-deoxyadenosine
pH 8.0, 22°C
Drosophila melanogaster
2.7.1.145
0.654
-
2'-deoxyguanosine
pH 8.0, 22°C
Drosophila melanogaster
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.145
30000
-
1 * 30000, SDS-PAGE
Drosophila melanogaster
2.7.1.145
33000
-
gel filtration
Drosophila melanogaster
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.145
-
Drosophila melanogaster
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.145
embryo
-
Drosophila melanogaster
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.145
29.4
-
-
Drosophila melanogaster
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.145
1-beta-D-arabinofuranosylcytosine + ATP
10% of the activity with thymidine and 2'-deoxycytidine
636840
Drosophila melanogaster
1-beta-D-arabinofuranosylcytosine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
1-beta-D-arabinofuranosylthymine + ATP
15% of the activity with thymidine and 2'-deoxycytidine
636840
Drosophila melanogaster
1-beta-D-arabinofuranosylthymine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2',3'-dideoxycytidine + ATP
0.02% of the activity with thymidine and 2'-deoxycytidine
636840
Drosophila melanogaster
2',3'-dideoxycytidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxyadenosine + ATP
5% of the activity with thymidine and 2'-deoxycytidine
636840
Drosophila melanogaster
2'-deoxyadenosine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxycytidine + ATP
-
636840
Drosophila melanogaster
2'-deoxycytidine 5'-phosphate + ADP
-
636840
Drosophila melanogaster
?
2.7.1.145
2'-deoxycytidine + ATP
-
636840
Drosophila melanogaster S-2
2'-deoxycytidine 5'-phosphate + ADP
-
636840
Drosophila melanogaster S-2
?
2.7.1.145
2'-deoxycytidine + CTP
-
636840
Drosophila melanogaster
2'-deoxycytidine 5'-phosphate + CDP
-
-
-
?
2.7.1.145
2'-deoxyguanosine + ATP
1% of the activity with thymidine and 2'-deoxycytidine
636840
Drosophila melanogaster
2'-deoxyguanosine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxyuridine + ATP
90% of the activity with thymidine and 2'-deoxycytidine
636840
Drosophila melanogaster
2'-deoxyuridine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
3'-azidothymidine + ATP
0.5% of the activity with thymidine and 2'-deoxycytidine
636840
Drosophila melanogaster
3'-azidothymidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
3'-fluoro-2',3'-dideoxythymidine + ATP
0.03% of the activity with thymidine and 2'-deoxycytidine
636840
Drosophila melanogaster
3'-fluoro-2',3'-dideoxythymidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
5-fluoro-2'-deoxyuridine + ATP
activity is 10% higher than with thymidine and 2'-deoxycytidine
636840
Drosophila melanogaster
5-fluoro-2'-deoxyuridine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
additional information
no activity with acyclovir
636840
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
additional information
activity with CTP is 110% of the activity ATP
636840
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
additional information
no activity with dTMP
636840
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
additional information
no activity with acyclovir
636840
Drosophila melanogaster S-2
?
-
-
-
-
2.7.1.145
additional information
activity with CTP is 110% of the activity ATP
636840
Drosophila melanogaster S-2
?
-
-
-
-
2.7.1.145
additional information
no activity with dTMP
636840
Drosophila melanogaster S-2
?
-
-
-
-
2.7.1.145
thymidine + ATP
-
636840
Drosophila melanogaster
thymidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
thymidine + ATP
-
636840
Drosophila melanogaster S-2
thymidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
thymidine + CTP
-
636840
Drosophila melanogaster
thymidine 5'-phosphate + CDP
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.145
monomer
1 * 30000, SDS-PAGE
Drosophila melanogaster
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.145
40
60
-
Drosophila melanogaster
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.145
15
-
2'-deoxycytidine
pH 8.0, 22°C
Drosophila melanogaster
2.7.1.145
15
-
thymidine
pH 8.0, 22°C
Drosophila melanogaster
2.7.1.145
18
19
2'-deoxyadenosine
pH 8.0, 22°C
Drosophila melanogaster
2.7.1.145
18
19
2'-deoxyguanosine
pH 8.0, 22°C
Drosophila melanogaster