EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.3.3 | Golgi membrane | less than 4% of sialyltransferase activity | Oncorhynchus mykiss | 139 | - |
2.4.3.3 | Golgi vesicle | in ovaries 96% of sialyltransferase activity is found in the Golgi-derived immature cortical vesicles or as soluble enzyme released from the cortical vesicles | Oncorhynchus mykiss | - |
- |
2.4.3.3 | soluble | in ovaries 96% of sialyltransferase activity is found in the Golgi-derived immature cortical vesicles or as soluble enzyme released from the cortical vesicles | Oncorhynchus mykiss | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.3.3 | CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | Oncorhynchus mykiss | required for synthesis of polySia side chains in trout egg polysialoglycoproteins, developmental expression pattern of enzyme in cortical vesicles during oogenesis, activity increases during oogenesis | CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.3.3 | Oncorhynchus mykiss | - |
rainbow trout | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.3.3 | egg | unfertilized mature eggs | Oncorhynchus mykiss | - |
2.4.3.3 | ovary | - |
Oncorhynchus mykiss | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.3.3 | CMP-N-acetylneuraminate + asialo-polysialoglycoprotein | from egg, Sia residue is alpha-2,6-linked to the proximal GalNAc residue in asialo-polysialoglycoprotein, acceptor carbohydrate unit: Galbeta(1-3)GalNAcalpha(1-O)-Ser/Thr, but not GalNAc1 to O-Ser/Thr | Oncorhynchus mykiss | CMP + alpha-sialosyl-(2-6)-asialo-polysialoglycoprotein | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | required for synthesis of polySia side chains in trout egg polysialoglycoproteins, developmental expression pattern of enzyme in cortical vesicles during oogenesis, activity increases during oogenesis | Oncorhynchus mykiss | CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + native polysialoglycoprotein | from egg, almost identical amount of Neu5Ac is incorporated into native and asialo-polysialoglycoprotein acceptor, acceptor carbohydrate unit: Galbeta(1-3)GalNAcalpha(1-O)-Ser/Thr, but not GalNAc1 to O-Ser/Thr | Oncorhynchus mykiss | ? | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | acceptor substrate specificity | Oncorhynchus mykiss | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | native and asialo-polysialoglycoprotein, acceptor carbohydrate unit: Galbeta(1-3)GalNAcalpha(1-O)-Ser/Thr, but not GalNAc1 to O-Ser/Thr | Oncorhynchus mykiss | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | CMP-N-glycolylneuraminate can replace CMP-N-acetylneuraminate | Oncorhynchus mykiss | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
2.4.3.3 | CMP-N-glycolylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | - |
Oncorhynchus mykiss | CMP + R-(2,6-alpha-N-glycolylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.3.3 | 22 | - |
assay at | Oncorhynchus mykiss |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.3.3 | 6 | 7 | acceptor: asialo-polysialoglycoprotein | Oncorhynchus mykiss |