Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gross, H.J.; Rose, U.; Krause, J.M.; Paulson, J.C.; Schmid, K.; Feeney, R.E.; Brossmer, R.
    Transfer of synthetic sialic acid analogues to N- and O-linked glycoprotein glycans using four different mammalian sialyltransferases (1989), Biochemistry, 28, 7386-7392.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.1 0.03
-
 CMP-9-benzamido-N-acetylneuraminate
-
 Rattus norvegicus
2.4.3.1 0.05
-
 CMP-N-acetylneuraminate with asialo-alpha1-acid glycoprotein Rattus norvegicus
2.4.3.1 0.12
-
 CMP-9-acetamido-N-acetylneuraminate
-
 Rattus norvegicus
2.4.3.1 0.72
-
 CMP-9-amino-N-acetylneuraminate
-
 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.1 Rattus norvegicus
-
 purified enzyme
-
2.4.3.3 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.1 liver
-
 Rattus norvegicus
-
2.4.3.3 submaxillary gland
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.3.1 additional information
-
 kinetics Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.1 CMP-9-acetamido-N-acetylneuraminate + asialo-alpha1-acid glycoprotein
-
 Rattus norvegicus ?
-
 ?
2.4.3.1 CMP-9-amino-N-acetylneuraminate + asialo-alpha1-acid glycoprotein
-
 Rattus norvegicus ?
-
 ?
2.4.3.1 CMP-9-azido-N-acetylneuraminate + asialo-alpha1-acid glycoprotein
-
 Rattus norvegicus ?
-
 ?
2.4.3.1 CMP-9-benzamido-N-acetylneuraminate + asialo-alpha1-acid glycoprotein
-
 Rattus norvegicus ?
-
 ?
2.4.3.1 CMP-9-hexanoylamido-N-acetylneuraminate + asialo-alpha1-acid glycoprotein
-
 Rattus norvegicus ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-ceruloplasmin
-
 Rattus norvegicus CMP + ceruloplasmin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-orosomucoid
-
 Rattus norvegicus CMP + orosomucoid
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R acceptor substrate specificity Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R transfers sialic acid to terminal positions on N-linked glycans Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R donor substrate specificity Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.3 CMP-9-acetamidoneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein less effective than CMP-N-acetylneuraminate Sus scrofa CMP + R-(2,6-9-acetamidoneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
2.4.3.3 CMP-9-aminoacetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein CMP-9-aminoacetylneuraminate can replace CMP-N-acetylneuraminate only with asialofetuin as acceptor Sus scrofa CMP + R-(2,6-9-aminoacetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
2.4.3.3 CMP-9-azido-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein CMP-9-azido-N-acetylneuraminate can replace CMP-N-acetylneuraminate with asialofetuin or antifreeze glycoprotein as acceptor Sus scrofa CMP + R-(2,6-9-azido-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
2.4.3.3 CMP-9-benzamidoneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein less effective than CMP-N-acetylneuraminate Sus scrofa CMP + R-(2,6-9-benzamidoneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
2.4.3.3 CMP-9-hexanoylamidoneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein less effective than CMP-N-acetylneuraminate Sus scrofa CMP + R-(2,6-9-hexanoylamidoneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
2.4.3.3 CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein asialo-fetuin Sus scrofa CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
2.4.3.3 CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein transfers sialic acid to the core region of O-linked glycans Sus scrofa CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
2.4.3.3 CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein CMP-9-acetamidoneuraminate, CMP-9-benzamidoneuraminate and CMP-9-hexanoylamidoneuraminate are less effective, CMP-9-azido-N-acetylneuraminate can replace CMP-N-acetylneuraminate with asialofetuin or antifreeze glycoprotein as acceptor, CMP-9-aminoacetylneuraminate only with asialofetuin as acceptor Sus scrofa CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
2.4.3.3 CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein antifreeze glycoprotein Sus scrofa CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
2.4.3.3 additional information no donor: CMP-9-amino-acetylneuraminate Sus scrofa ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.3.1 37
-
 assay at Rattus norvegicus