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Literature summary extracted from

  • Jensen, J.W.; Schutzbach, J.S.
    The biosynthesis of oligosaccharide-lipids. Activation of mannosyltransferase II by specific phospholipids (1982), J. Biol. Chem., 257, 9025-9029.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.132 cardiolipin activation, 35% as effective as phosphatidylethanolamine Oryctolagus cuniculus
2.4.1.132 phosphatidylethanolamine activation Oryctolagus cuniculus
2.4.1.132 phosphatidylethanolamine and mixtures with other phospholipids forming non-bilayer phospholipid phases e.g. hexagonal phases Oryctolagus cuniculus
2.4.1.132 phosphatidylethanolamine no activation by phosphatidylcholine, phosphatidylinositol, phosphatidylglycerol, sphingomyelin, lysophosphocholine or lysophosphoethanolamine Oryctolagus cuniculus
2.4.1.132 phosphatidylethanolamine phosphoethanolamine containing unsaturated acylchains Oryctolagus cuniculus
2.4.1.132 phosphatidylethanolamine in the absence of detergent Oryctolagus cuniculus
2.4.1.132 phosphatidylethanolamine enzyme-phospholipid-complex formation Oryctolagus cuniculus
2.4.1.132 phosphatidylethanolamine from various eukaryotic and prokaryotic sources Oryctolagus cuniculus
2.4.1.132 phosphatidylserine activation, 16-43% as effective as phosphatidylethanolamine Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.132 0.00096
-
 tetrasaccharide-diphosphoryl-lipid
-
 Oryctolagus cuniculus
2.4.1.132 0.00142
-
 GDPmannose
-
 Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.132 microsome
-
 Oryctolagus cuniculus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.132 Mg2+ requirement Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.132 Oryctolagus cuniculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.132 liver
-
 Oryctolagus cuniculus
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.132 -20°C, in 0.1% v/v Nonidet P-40 and 10% v/v glycerol, at least 3 months Oryctolagus cuniculus
2.4.1.132 0-4°C, in 0.1% v/v Nonidet P-40 and 10% v/v glycerol, 24 h Oryctolagus cuniculus
2.4.1.132 glycerol, 10% v/v, stabilizes during storage Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.132 GDPmannose + tetrasaccharide-diphosphoryl-lipid
-
 Oryctolagus cuniculus GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.132 37
-
 assay at Oryctolagus cuniculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.132 additional information
-
 phosphatidylethanolamine increases heat-stability Oryctolagus cuniculus
2.4.1.132 37
-
 5 min, in 0.0225% Nonidet P-40 without substrate, about 80% loss of activity. In phosphatidylethanolamine without substrate: at least 5 min stable, after 25 min about 15% loss of activity Oryctolagus cuniculus