BRENDA - Enzyme Database

The biosynthesis of oligosaccharide-lipids. Activation of mannosyltransferase II by specific phospholipids

Jensen, J.W.; Schutzbach, J.S.; J. Biol. Chem. 257, 9025-9029 (1982)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.132
cardiolipin
activation, 35% as effective as phosphatidylethanolamine
Oryctolagus cuniculus
2.4.1.132
phosphatidylethanolamine
activation; and mixtures with other phospholipids forming non-bilayer phospholipid phases e.g. hexagonal phases; enzyme-phospholipid-complex formation; from various eukaryotic and prokaryotic sources; in the absence of detergent; no activation by phosphatidylcholine, phosphatidylinositol, phosphatidylglycerol, sphingomyelin, lysophosphocholine or lysophosphoethanolamine; phosphoethanolamine containing unsaturated acylchains
Oryctolagus cuniculus
2.4.1.132
phosphatidylserine
activation, 16-43% as effective as phosphatidylethanolamine
Oryctolagus cuniculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.132
0.00096
-
tetrasaccharide-diphosphoryl-lipid
-
Oryctolagus cuniculus
2.4.1.132
0.00142
-
GDPmannose
-
Oryctolagus cuniculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.132
microsome
-
Oryctolagus cuniculus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.132
Mg2+
requirement
Oryctolagus cuniculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.132
Oryctolagus cuniculus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.132
liver
-
Oryctolagus cuniculus
-
Storage Stability
EC Number
Storage Stability
Organism
2.4.1.132
-20°C, in 0.1% v/v Nonidet P-40 and 10% v/v glycerol, at least 3 months
Oryctolagus cuniculus
2.4.1.132
0-4°C, in 0.1% v/v Nonidet P-40 and 10% v/v glycerol, 24 h
Oryctolagus cuniculus
2.4.1.132
glycerol, 10% v/v, stabilizes during storage
Oryctolagus cuniculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.132
GDPmannose + tetrasaccharide-diphosphoryl-lipid
-
636754
Oryctolagus cuniculus
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
636754
Oryctolagus cuniculus
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.132
37
-
assay at
Oryctolagus cuniculus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.4.1.132
additional information
-
phosphatidylethanolamine increases heat-stability
Oryctolagus cuniculus
2.4.1.132
37
-
5 min, in 0.0225% Nonidet P-40 without substrate, about 80% loss of activity. In phosphatidylethanolamine without substrate: at least 5 min stable, after 25 min about 15% loss of activity
Oryctolagus cuniculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.132
cardiolipin
activation, 35% as effective as phosphatidylethanolamine
Oryctolagus cuniculus
2.4.1.132
phosphatidylethanolamine
activation; and mixtures with other phospholipids forming non-bilayer phospholipid phases e.g. hexagonal phases; enzyme-phospholipid-complex formation; from various eukaryotic and prokaryotic sources; in the absence of detergent; no activation by phosphatidylcholine, phosphatidylinositol, phosphatidylglycerol, sphingomyelin, lysophosphocholine or lysophosphoethanolamine; phosphoethanolamine containing unsaturated acylchains
Oryctolagus cuniculus
2.4.1.132
phosphatidylserine
activation, 16-43% as effective as phosphatidylethanolamine
Oryctolagus cuniculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.132
0.00096
-
tetrasaccharide-diphosphoryl-lipid
-
Oryctolagus cuniculus
2.4.1.132
0.00142
-
GDPmannose
-
Oryctolagus cuniculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.132
microsome
-
Oryctolagus cuniculus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.132
Mg2+
requirement
Oryctolagus cuniculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.132
liver
-
Oryctolagus cuniculus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.4.1.132
-20°C, in 0.1% v/v Nonidet P-40 and 10% v/v glycerol, at least 3 months
Oryctolagus cuniculus
2.4.1.132
0-4°C, in 0.1% v/v Nonidet P-40 and 10% v/v glycerol, 24 h
Oryctolagus cuniculus
2.4.1.132
glycerol, 10% v/v, stabilizes during storage
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.132
GDPmannose + tetrasaccharide-diphosphoryl-lipid
-
636754
Oryctolagus cuniculus
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
636754
Oryctolagus cuniculus
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.132
37
-
assay at
Oryctolagus cuniculus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.4.1.132
additional information
-
phosphatidylethanolamine increases heat-stability
Oryctolagus cuniculus
2.4.1.132
37
-
5 min, in 0.0225% Nonidet P-40 without substrate, about 80% loss of activity. In phosphatidylethanolamine without substrate: at least 5 min stable, after 25 min about 15% loss of activity
Oryctolagus cuniculus