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Literature summary extracted from

  • Orzechowski, S.; Socha-Hanc, J.; Paszkowski, A.
    Alanine aminotransferase and glycine aminotransferase from maize (Zea mays L.) leaves (1999), Acta Biochim. Pol., 46, 447-457.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.2 3-chloro-L-alanine 0.1 mM, 78% inhibition Zea mays
2.6.1.2 Cd2+ 0.1 mM, complete inhibition Zea mays
2.6.1.2 cycloserine 0.1 mM, 98% inhibition Zea mays
2.6.1.2 p-hydroxymercuribenzoate 0.1 mM, 72% inhibition Zea mays
2.6.1.2 Zn2+ 1 mM, 75% inhibition Zea mays
2.6.1.4 3-chloro-L-alanine
-
 Zea mays
2.6.1.4 Aminooxyacetate
-
 Zea mays
2.6.1.4 Cd2+ 85% inhibition at 1 mM Zea mays
2.6.1.4 L-alanine competitive Zea mays
2.6.1.4 additional information p-hydroxymercuribenzoate and cycloserine are poor inhibitors Zea mays
2.6.1.4 Zn2+ weak, 25% inhibition at 1 mM Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.4 50000
-
 5 isoforms, gel filtration Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.4 glyoxylate + L-glutamate Zea mays
-
 glycine + 2-oxoglutarate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.2 Zea mays
-
-
-
2.6.1.4 Zea mays
-
 5 isoforms with varying substrate specificities
-
2.6.1.4 Zea mays
-
 var. Duet
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.2 ammonium sulfate, hydroxylapatite, preparative electrophoresis Zea mays
2.6.1.4 partial, 5 isoforms Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.4 leaf
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.2 1.39
-
-
 Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.4 glyoxylate + L-alanine
-
 Zea mays glycine + pyruvate
-
 ?
2.6.1.4 glyoxylate + L-glutamate
-
 Zea mays glycine + 2-oxoglutarate
-
 ?
2.6.1.4 additional information substrate specificities of isoforms Zea mays ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.4 GlyAT
-
 Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.4 30
-
 assay at Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.4 7.5
-
 assay at Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.4 pyridoxal 5'-phosphate
-
 Zea mays