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Literature summary extracted from

  • Good, A.G.; Muench, D.G.
    Purification and characterization of an anaerobically induced alanine aminotransferase from barley roots (1992), Plant Physiol., 99, 1520-1525.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.2 Aminooxyacetate 1 mM, 98% inhibition Hordeum vulgare
2.6.1.2 Glutarate 10 mM, 94% inhibition Hordeum vulgare
2.6.1.2 Semicarbazide 10 mM, 97% inhibition Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.2 0.06
-
 pyruvate pH 8.0, 23°C Hordeum vulgare
2.6.1.2 1
-
 glutamate pH 8.0, 23°C Hordeum vulgare
2.6.1.2 5
-
 2-oxoglutarate pH 8.0, 23°C Hordeum vulgare
2.6.1.2 17
-
 alanine pH 8.0, 23°C Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.2 50000
-
 2 * 50000, SDS-PAGE Hordeum vulgare
2.6.1.2 97000
-
 gel filtration Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.2 Hordeum vulgare
-
 4 isoforms
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.2 anaerobically induced isoform, ammonium sulfate, gel filtration, Q-Sepharose, Mono P, Mono Q Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.2 root
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.2 2231
-
-
 Hordeum vulgare

Storage Stability

EC Number Storage Stability Organism
2.6.1.2 -20°C, 10% glycerol, half-life of 2 weeks Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.2 L-alanine + 2-oxoglutarate highly specific for alanine and 2-oxoglutarate Hordeum vulgare pyruvate + L-glutamate
-
 ?
2.6.1.2 L-alanine + 2-oxoglutarate cannot use glyoxylate as keto donor Hordeum vulgare pyruvate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.2 dimer 2 * 50000, SDS-PAGE Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.2 8 8.3 with alanine and 2-oxoglutarate Hordeum vulgare

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.2 additional information anaerobically induced isoform does not require the presence of pyridoxal 5'-phosphate to retain its activity Hordeum vulgare