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Literature summary extracted from

  • Lain-Guelbenzu, B.; Cardenas, J.; Munoz-Blanco, J.
    Purification and properties of L-alanine aminotransferase from Chlamydomonas reinhardtii (1991), Eur. J. Biochem., 202, 881-887.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.2 pyridoxal 5'-phosphate, L-alanine or 2-oxoglutarate protects against heat inactivation Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.2 1,10-phenanthroline 2 mM, 51% inhibition Chlamydomonas reinhardtii
2.6.1.2 Aminooxyacetate 1 mM, complete inhibition Chlamydomonas reinhardtii
2.6.1.2 hydroxylamine 2 mM, 96% inhibition Chlamydomonas reinhardtii
2.6.1.2 L-ascorbic acid 2 mM, 25% inhibition Chlamydomonas reinhardtii
2.6.1.2 p-hydroxymercuribenzoate 1 mM, complete inhibition Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.2 0.05
-
 2-oxoglutarate pH 7.3, 40°C Chlamydomonas reinhardtii
2.6.1.2 0.24
-
 pyruvate pH 7.3, 40°C Chlamydomonas reinhardtii
2.6.1.2 0.52
-
 L-glutamate pH 7.3, 40°C Chlamydomonas reinhardtii
2.6.1.2 2.7
-
 L-alanine pH 7.3, 40°C Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.2 45000
-
 2 * 45000, SDS-PAGE Chlamydomonas reinhardtii
2.6.1.2 105000
-
 gel filtration Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.2 Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.2
-
 Chlamydomonas reinhardtii

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.2 L-alanine + 2-oxoglutarate = pyruvate + L-glutamate ping pong bi bi mechanism Chlamydomonas reinhardtii
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.2 18.66
-
-
 Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.2 glyoxylate + alanine
-
 Chlamydomonas reinhardtii glycine + pyruvate
-
 ?
2.6.1.2 glyoxylate + glutamate
-
 Chlamydomonas reinhardtii glycine + 2-oxoglutarate
-
 ?
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Chlamydomonas reinhardtii pyruvate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.2 dimer 2 * 45000, SDS-PAGE Chlamydomonas reinhardtii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.2 50
-
-
 Chlamydomonas reinhardtii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.2 additional information
-
 pyridoxal 5-phosphate, L-alanine or 2-oxoglutarate protect against heat inactivation Chlamydomonas reinhardtii
2.6.1.2 50
-
 50% loss of activity after 10 min Chlamydomonas reinhardtii
2.6.1.2 60
-
 more than 85% loss of activity after 5 min Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.2 7.3
-
-
 Chlamydomonas reinhardtii

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.2 pyridoxal 5'-phosphate a pyridoxal phosphate protein, 2 mol pyridoxal phosphate per mol of enzyme Chlamydomonas reinhardtii