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Literature summary extracted from

  • Owen, T.G.; Hochachka, P.W.
    Purification and properties of dolphin muscle aspartate and alanine transaminases and thier possible roles in the energy metabolism of diving mammals (1974), Biochem. J., 143, 541-553.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.1 2-oxoglutarate oxoglutarate production; substrate inhibition at high concentration dolphin
2.6.1.1 L-glutamate forward reaction, substrate L-aspartate dolphin
2.6.1.1 malate isozymes Asp-DEAE-1 and Asp-DEAE-2 dolphin
2.6.1.1 oxaloacetate substrate inhibition dolphin
2.6.1.1 succinate forward reaction dolphin

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.1 additional information
-
 additional information
-
 dolphin
2.6.1.2 0.45
-
 2-oxoglutarate pH 7.3, 37°C Lagenorhynchus obliquidens
2.6.1.2 0.87
-
 pyruvate pH 7.3, 37°C Lagenorhynchus obliquidens
2.6.1.2 8.2
-
 L-alanine pH 7.3, 37°C Lagenorhynchus obliquidens
2.6.1.2 15
-
 glutamate pH 7.3, 37°C Lagenorhynchus obliquidens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.1 cytosol cytosolic isoenzyme dolphin 5829
-
2.6.1.1 mitochondrion mitochondrial isoenzyme dolphin 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate dolphin role in energy metabolism of diving animals oxaloacetate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.1 dolphin
-
 cytosolic and mitochondrial isozyme
-
2.6.1.2 Lagenorhynchus obliquidens
-
 dolphin
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.1 cytosolic and mitochondrial isozymes dolphin
2.6.1.2
-
 Lagenorhynchus obliquidens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.1 muscle
-
 dolphin
-
2.6.1.2 muscle
-
 Lagenorhynchus obliquidens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.1 100
-
 purified cytosolic isozyme dolphin
2.6.1.1 228
-
 purified mitochondrial isozyme dolphin
2.6.1.2 35.5
-
-
 Lagenorhynchus obliquidens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 dolphin oxaloacetate + L-glutamate
-
 r
2.6.1.1 L-aspartate + 2-oxoglutarate role in energy metabolism of diving animals dolphin oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-glutamate + 2-oxoglutarate L-glutamate is best amino acid donor dolphin 2-oxoglutarate + L-glutamate
-
 ?
2.6.1.1 L-phenylalanine + 2-oxoglutarate
-
 dolphin 2-oxo-3-phenylpropionic acid + L-glutamate
-
 ir
2.6.1.1 L-tyrosine + 2-oxoglutarate
-
 dolphin 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate
-
 ir
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Lagenorhynchus obliquidens pyruvate + L-glutamate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.2 37
-
 assay at Lagenorhynchus obliquidens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.1 6.3 7.3 mitochondrial enzyme dolphin
2.6.1.1 7.3 8.3 cytosolic enzyme dolphin
2.6.1.2 7.2 7.8
-
 Lagenorhynchus obliquidens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.1 6.8 8.3 pH 6.8: 76% of activity maximum, pH 7.3-8.3: activity maximum, supernatant enzyme dolphin
2.6.1.2 6.3 7.8 approx. 40% of maximal activity at pH 6.3, 75% of maximal activity at pH 6.8 Lagenorhynchus obliquidens