EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.45 | 3-chloro-L-alanine | - |
Hyphomicrobium methylovorum | |
2.6.1.45 | AgNO3 | strong inhibition | Hyphomicrobium methylovorum | |
2.6.1.45 | cycloserine | weak inhibition | Hyphomicrobium methylovorum | |
2.6.1.45 | HgCl2 | strong inhibition | Hyphomicrobium methylovorum | |
2.6.1.45 | hydroxylamine | - |
Hyphomicrobium methylovorum | |
2.6.1.45 | iodoacetamide | - |
Hyphomicrobium methylovorum | |
2.6.1.45 | KCN | weak inhibition | Hyphomicrobium methylovorum | |
2.6.1.45 | MgCl2 | weak inhibition | Hyphomicrobium methylovorum | |
2.6.1.45 | additional information | not inhibited by NaBH4, 3-chloro-D-alanine, 1,10-phenanthroline, 2,2'-dipyridyl, 8-hydroxyquinoline, EDTA, iodoacetate, FeCl3, AlCl3, CdCl2, CoCl2, NiCl2, CaCl2, NaN3, ZnCl2, PbCl2, LiCl, CuCl2, BaCl2, ascorbate, cysteamine, N-ethylmaleimide | Hyphomicrobium methylovorum | |
2.6.1.45 | NaF | weak inhibition | Hyphomicrobium methylovorum | |
2.6.1.45 | p-chloromercuribenzoate | - |
Hyphomicrobium methylovorum | |
2.6.1.45 | Penicillamine | weak inhibition | Hyphomicrobium methylovorum | |
2.6.1.45 | phenylhydrazine | weak inhibition | Hyphomicrobium methylovorum | |
2.6.1.45 | Semicarbazide | - |
Hyphomicrobium methylovorum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.45 | 0.23 | - |
glyoxylate | pH 7.0, 30°C | Hyphomicrobium methylovorum | |
2.6.1.45 | 4.98 | - |
L-serine | - |
Hyphomicrobium methylovorum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.6.1.45 | soluble | - |
Hyphomicrobium methylovorum | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.6.1.45 | 40000 | - |
4 * 40000, SDS-PAGE | Hyphomicrobium methylovorum |
2.6.1.45 | 140000 | - |
HPLC, gel filtration | Hyphomicrobium methylovorum |
2.6.1.45 | 150000 | - |
Sephacryl S-200, gel filtration | Hyphomicrobium methylovorum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.45 | L-serine + glyoxylate | Hyphomicrobium methylovorum | plays essential role in methanol assimilation through serine pathway | 3-hydroxypyruvate + glycine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.45 | Hyphomicrobium methylovorum | - |
GM2, glycine-resistant mutant derived from parent strain KM146 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.45 | ammonium sulfate, DEAE-Sephacel, phenyl-Sepharose, CM-toyopearl, Cellulofine | Hyphomicrobium methylovorum |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.6.1.45 | L-serine + glyoxylate = 3-hydroxypyruvate + glycine | ping-pong reaction mechanism | Hyphomicrobium methylovorum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.6.1.45 | 69.5 | - |
- |
Hyphomicrobium methylovorum |
EC Number | Storage Stability | Organism |
---|---|---|
2.6.1.45 | -20°C, 10 mM phosphate buffer pH 7, 0.1 mM pyridoxal phosphate, 0.1 mM dithiothreitol, 45% v/v glycerol, at least 5 months | Hyphomicrobium methylovorum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.45 | L-serine + 2-oxomalonate | - |
Hyphomicrobium methylovorum | 3-hydroxypyruvate + 2-aminomalonate | - |
? | |
2.6.1.45 | L-serine + glyoxylate | highly specific | Hyphomicrobium methylovorum | 3-hydroxypyruvate + glycine | - |
ir | |
2.6.1.45 | L-serine + glyoxylate | no activity with Phe, Arg, Val, Trp, Thr, Met | Hyphomicrobium methylovorum | 3-hydroxypyruvate + glycine | - |
ir | |
2.6.1.45 | L-serine + glyoxylate | nearly irreversible, trace amounts of L-serine | Hyphomicrobium methylovorum | 3-hydroxypyruvate + glycine | - |
ir | |
2.6.1.45 | L-serine + glyoxylate | no activity with D-serine | Hyphomicrobium methylovorum | 3-hydroxypyruvate + glycine | - |
ir | |
2.6.1.45 | L-serine + glyoxylate | no or trace activity with oxaloacetate, 2-oxoglutarate | Hyphomicrobium methylovorum | 3-hydroxypyruvate + glycine | - |
ir | |
2.6.1.45 | L-serine + glyoxylate | no activity with Ala, Glu, Gln, His, oxamate, 2-oxo-n-butanoate, 3-methyl-2-oxo-butanoate, 2-methyl-DL-serine, L-serine-O-sulfate, DL-serine hydroxamate, O-phospho-L-serine | Hyphomicrobium methylovorum | 3-hydroxypyruvate + glycine | - |
ir | |
2.6.1.45 | L-serine + glyoxylate | plays essential role in methanol assimilation through serine pathway | Hyphomicrobium methylovorum | 3-hydroxypyruvate + glycine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.6.1.45 | tetramer | 4 * 40000, SDS-PAGE | Hyphomicrobium methylovorum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.45 | 40 | - |
- |
Hyphomicrobium methylovorum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.45 | 35 | - |
and below, 30 min stable | Hyphomicrobium methylovorum |
2.6.1.45 | 40 | - |
30 min, 16% loss of activity | Hyphomicrobium methylovorum |
2.6.1.45 | 45 | - |
52% loss of activity after 30 min | Hyphomicrobium methylovorum |
2.6.1.45 | 50 | - |
30 min, 93% loss of activity | Hyphomicrobium methylovorum |
2.6.1.45 | 55 | - |
30 min, inactivation | Hyphomicrobium methylovorum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.45 | additional information | - |
pI: 6.9 | Hyphomicrobium methylovorum |
2.6.1.45 | 8 | - |
- |
Hyphomicrobium methylovorum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.45 | 6 | 11 | stable for 30 min at 30°C | Hyphomicrobium methylovorum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.45 | pyridoxal 5'-phosphate | required for activity | Hyphomicrobium methylovorum | |
2.6.1.45 | pyridoxal 5'-phosphate | a pyridoxal phosphate protein, 4 mol per mol enzyme | Hyphomicrobium methylovorum |