Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Izumi, Y.; Yoshida, T.; Yamada, H.
    Purification and characterization of serine-glyoxylate aminotransferase from a serine-producing methylotroph, Hyphomicrobium methylovorum GM2 (1990), Eur. J. Biochem., 190, 285-290.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.45 3-chloro-L-alanine
-
 Hyphomicrobium methylovorum
2.6.1.45 AgNO3 strong inhibition Hyphomicrobium methylovorum
2.6.1.45 cycloserine weak inhibition Hyphomicrobium methylovorum
2.6.1.45 HgCl2 strong inhibition Hyphomicrobium methylovorum
2.6.1.45 hydroxylamine
-
 Hyphomicrobium methylovorum
2.6.1.45 iodoacetamide
-
 Hyphomicrobium methylovorum
2.6.1.45 KCN weak inhibition Hyphomicrobium methylovorum
2.6.1.45 MgCl2 weak inhibition Hyphomicrobium methylovorum
2.6.1.45 additional information not inhibited by NaBH4, 3-chloro-D-alanine, 1,10-phenanthroline, 2,2'-dipyridyl, 8-hydroxyquinoline, EDTA, iodoacetate, FeCl3, AlCl3, CdCl2, CoCl2, NiCl2, CaCl2, NaN3, ZnCl2, PbCl2, LiCl, CuCl2, BaCl2, ascorbate, cysteamine, N-ethylmaleimide Hyphomicrobium methylovorum
2.6.1.45 NaF weak inhibition Hyphomicrobium methylovorum
2.6.1.45 p-chloromercuribenzoate
-
 Hyphomicrobium methylovorum
2.6.1.45 Penicillamine weak inhibition Hyphomicrobium methylovorum
2.6.1.45 phenylhydrazine weak inhibition Hyphomicrobium methylovorum
2.6.1.45 Semicarbazide
-
 Hyphomicrobium methylovorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.45 0.23
-
 glyoxylate pH 7.0, 30°C Hyphomicrobium methylovorum
2.6.1.45 4.98
-
 L-serine
-
 Hyphomicrobium methylovorum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.45 soluble
-
 Hyphomicrobium methylovorum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.45 40000
-
 4 * 40000, SDS-PAGE Hyphomicrobium methylovorum
2.6.1.45 140000
-
 HPLC, gel filtration Hyphomicrobium methylovorum
2.6.1.45 150000
-
 Sephacryl S-200, gel filtration Hyphomicrobium methylovorum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.45 L-serine + glyoxylate Hyphomicrobium methylovorum plays essential role in methanol assimilation through serine pathway 3-hydroxypyruvate + glycine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.45 Hyphomicrobium methylovorum
-
 GM2, glycine-resistant mutant derived from parent strain KM146
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.45 ammonium sulfate, DEAE-Sephacel, phenyl-Sepharose, CM-toyopearl, Cellulofine Hyphomicrobium methylovorum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.45 L-serine + glyoxylate = 3-hydroxypyruvate + glycine ping-pong reaction mechanism Hyphomicrobium methylovorum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.45 69.5
-
-
 Hyphomicrobium methylovorum

Storage Stability

EC Number Storage Stability Organism
2.6.1.45 -20°C, 10 mM phosphate buffer pH 7, 0.1 mM pyridoxal phosphate, 0.1 mM dithiothreitol, 45% v/v glycerol, at least 5 months Hyphomicrobium methylovorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.45 L-serine + 2-oxomalonate
-
 Hyphomicrobium methylovorum 3-hydroxypyruvate + 2-aminomalonate
-
 ?
2.6.1.45 L-serine + glyoxylate highly specific Hyphomicrobium methylovorum 3-hydroxypyruvate + glycine
-
 ir
2.6.1.45 L-serine + glyoxylate no activity with Phe, Arg, Val, Trp, Thr, Met Hyphomicrobium methylovorum 3-hydroxypyruvate + glycine
-
 ir
2.6.1.45 L-serine + glyoxylate nearly irreversible, trace amounts of L-serine Hyphomicrobium methylovorum 3-hydroxypyruvate + glycine
-
 ir
2.6.1.45 L-serine + glyoxylate no activity with D-serine Hyphomicrobium methylovorum 3-hydroxypyruvate + glycine
-
 ir
2.6.1.45 L-serine + glyoxylate no or trace activity with oxaloacetate, 2-oxoglutarate Hyphomicrobium methylovorum 3-hydroxypyruvate + glycine
-
 ir
2.6.1.45 L-serine + glyoxylate no activity with Ala, Glu, Gln, His, oxamate, 2-oxo-n-butanoate, 3-methyl-2-oxo-butanoate, 2-methyl-DL-serine, L-serine-O-sulfate, DL-serine hydroxamate, O-phospho-L-serine Hyphomicrobium methylovorum 3-hydroxypyruvate + glycine
-
 ir
2.6.1.45 L-serine + glyoxylate plays essential role in methanol assimilation through serine pathway Hyphomicrobium methylovorum 3-hydroxypyruvate + glycine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.45 tetramer 4 * 40000, SDS-PAGE Hyphomicrobium methylovorum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.45 40
-
-
 Hyphomicrobium methylovorum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.45 35
-
 and below, 30 min stable Hyphomicrobium methylovorum
2.6.1.45 40
-
 30 min, 16% loss of activity Hyphomicrobium methylovorum
2.6.1.45 45
-
 52% loss of activity after 30 min Hyphomicrobium methylovorum
2.6.1.45 50
-
 30 min, 93% loss of activity Hyphomicrobium methylovorum
2.6.1.45 55
-
 30 min, inactivation Hyphomicrobium methylovorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.45 additional information
-
 pI: 6.9 Hyphomicrobium methylovorum
2.6.1.45 8
-
-
 Hyphomicrobium methylovorum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.6.1.45 6 11 stable for 30 min at 30°C Hyphomicrobium methylovorum

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.45 pyridoxal 5'-phosphate required for activity Hyphomicrobium methylovorum
2.6.1.45 pyridoxal 5'-phosphate a pyridoxal phosphate protein, 4 mol per mol enzyme Hyphomicrobium methylovorum