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Literature summary extracted from

  • Fonnum, F.; Larsen, K.
    Purification and properties of dihydroxyphenylalanine transaminase from guinea pig brain (1965), J. Neurochem., 12, 589-598.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.6.1.49 pyridoxal phosphate a pyridoxal phosphate protein, Km: 0.016 mM Cavia porcellus

General Stability

EC Number General Stability Organism
2.6.1.49 pyridoxal phosphate, Ca2+, Mg2+ or Mn2+ protects against heat inactivation Cavia porcellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.49 iodoacetic acid 1 mM, 75% inhibition Cavia porcellus
2.6.1.49 KCN 1 mM, 93% inhibition, pyridoxal 5'-phosphate protects Cavia porcellus
2.6.1.49 p-chloromercuriphenyl sulfonic acid 0.02 mM, 92% inhibition, addition of 1 mM glutathione protects to 100% Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.49 0.48
-
 2-oxoglutarate pH 8.2, 37°C Cavia porcellus
2.6.1.49 41
-
 L-Dopa pH 8.2, 37°C Cavia porcellus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.49 Cavia porcellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.49 ammonium sulfate, 52°C, calcium phosphate gel, alumina C, Sephadex G-200 Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.49 brain
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.49 0.0366
-
-
 Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.49 3-(3,4-dihydroxyphenyl)-L-alanine + 2-oxoglutarate i.e. L-dopa Cavia porcellus 3-(3,4-dihydroxyphenyl)pyruvate + L-glutamate i.e. DOPP ?
2.6.1.49 3-(3,4-dihydroxyphenyl)-L-alanine + 2-oxoglutarate most effective amino acceptor Cavia porcellus 3-(3,4-dihydroxyphenyl)pyruvate + L-glutamate i.e. DOPP ?
2.6.1.49 3-(3,4-dihydroxyphenyl)-L-alanine + oxaloacetic acid i.e. L-Dopa, 10% of activity with 2-oxoglutarate Cavia porcellus 3-(3,4-dihydroxyphenyl)pyruvate + L-aspartate
-
 ?
2.6.1.49 L-tyrosine + 2-oxoglutarate 8% of activity with L-Dopa Cavia porcellus 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.49 37
-
 assay at Cavia porcellus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.49 additional information
-
 pyridoxal phosphate, Ca2+, Mg2+ or Mn2+ protects against heat inactivation Cavia porcellus
2.6.1.49 55
-
 6 min, pH 7.0, stable Cavia porcellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.49 8.2
-
-
 Cavia porcellus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.49 7 9 approx. 35% of maximal activity at pH 7.0, approx. 60% of activity at pH 9.0 Cavia porcellus