Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Oue, S.; Okamoto, A.; Nakai, Y.; Nakahira, M.; Shibatani, T.; Hayashi, H.; Kagamiyama, H.
    Paracoccus denitrificans aromatic amino acid aminotransferase: a model enzyme for the study of dual substrate recognition mechanism (1997), J. Biochem., 121, 161-171.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.57 expression in Escherichia coli Paracoccus denitrificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.57 0.059
-
 3-(4-hydroxyphenyl)pyruvate pH 8.0, 25°C, cosubstrate aspartate Paracoccus denitrificans
2.6.1.57 0.083
-
 phenylpyruvate pH 8.0, 25°C, cosubstrate aspartate Paracoccus denitrificans
2.6.1.57 0.14
-
 2-oxoglutarate pH 8.0, 25°C, cosubstrate tryptophan Paracoccus denitrificans
2.6.1.57 0.59
-
 2-oxoglutarate pH 8.0, 25°C, cosubstrate aspartate Paracoccus denitrificans
2.6.1.57 1.4
-
 L-tryptophan pH 8.0, 25°C, cosubstrate 2-oxoglutarate Paracoccus denitrificans
2.6.1.57 1.8
-
 L-aspartate pH 8.0, 25°C, cosubstrate 2-oxoglutarate Paracoccus denitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.57 42653
-
 2 * 42653, mass spectrometry Paracoccus denitrificans
2.6.1.57 42731
-
 2 * 42731, deduced from nucleotide sequence Paracoccus denitrificans
2.6.1.57 83000
-
 gel filtration Paracoccus denitrificans

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.57 Paracoccus denitrificans P95468
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.57 ammonium sulfate, phenyl-Toyopearl, hydroxyapatite, Sephacryl S-200, recombinant enzyme Paracoccus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.57 aspartate + 3-phenylpyruvate
-
 Paracoccus denitrificans oxaloacetate + L-phenylalanine
-
 r
2.6.1.57 L-aspartate + 2-oxoglutarate
-
 Paracoccus denitrificans oxaloacetate + L-glutamate
-
 r
2.6.1.57 L-aspartate + 3-(4-hydroxyphenyl)pyruvate
-
 Paracoccus denitrificans L-tyrosine + oxaloacetate
-
 r
2.6.1.57 L-phenylalanine + 2-oxoglutarate enzyme also reacts with 2-aminooctanoate and 2-aminoheptanoate Paracoccus denitrificans phenylpyruvate + L-glutamate
-
 r
2.6.1.57 L-tryptophan + 2-oxoglutarate
-
 Paracoccus denitrificans 3-indole-2-oxopropanoate + L-glutamate
-
 r
2.6.1.57 L-tyrosine + 2-oxoglutarate
-
 Paracoccus denitrificans p-hydroxyphenylpyruvate + L-glutamate
-
 r

Subunits

EC Number Subunits Comment Organism
2.6.1.57 dimer 2 * 42731, deduced from nucleotide sequence Paracoccus denitrificans
2.6.1.57 dimer 2 * 42653, mass spectrometry Paracoccus denitrificans

Synonyms

EC Number Synonyms Comment Organism
2.6.1.57 pdArAT
-
 Paracoccus denitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.57 pyridoxal 5'-phosphate 1 pyridoxal 5'-phosphate per subunit Paracoccus denitrificans