EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.6.1.57 | R292A | very low activity with aspartate, 5-10fold increase in Km for aromatic amino acids | Escherichia coli |
2.6.1.57 | R292K | very low activity with aspartate, 10-100fold increase in Km for aromatic amino acids | Escherichia coli |
2.6.1.57 | R292L | very low activity with aspartate, 5-10fold increase in Km for aromatic amino acids | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.57 | 0.68 | - |
L-tryptophan | pH 8.0, 25°C, single-turnover, wild-type enzyme | Escherichia coli | |
2.6.1.57 | 0.83 | - |
L-tyrosine | pH 8.0, 25°C, single-turnover, wild-type enzyme | Escherichia coli | |
2.6.1.57 | 1 | - |
L-phenylalanine | pH 8.0, 25°C, single-turnover, wild-type enzyme | Escherichia coli | |
2.6.1.57 | 5 | - |
L-aspartate | pH 8.0, 25°C, single-turnover, wild-type enzyme | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.57 | Escherichia coli | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.57 | aspartate + 2-oxoglutarate | - |
Escherichia coli | oxaloacetate + L-glutamate | - |
r | |
2.6.1.57 | L-phenylalanine + 2-oxoglutarate | - |
Escherichia coli | phenylpyruvate + L-glutamate | - |
? | |
2.6.1.57 | L-tryptophan + 2-oxoglutarate | - |
Escherichia coli | 3-indole-2-oxopropanoate + L-glutamate | - |
? | |
2.6.1.57 | L-tyrosine + 2-oxoglutarate | - |
Escherichia coli | p-hydroxyphenylpyruvate + L-glutamate | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.57 | 290 | - |
L-aspartate | pH 8.0, 25°C, single-turnover, wild-type enzyme | Escherichia coli | |
2.6.1.57 | 350 | - |
L-tryptophan | pH 8.0, 25°C, single-turnover, wild-type enzyme | Escherichia coli | |
2.6.1.57 | 500 | - |
L-tyrosine | pH 8.0, 25°C, single-turnover, wild-type enzyme | Escherichia coli | |
2.6.1.57 | 1200 | - |
L-phenylalanine | pH 8.0, 25°C, single-turnover, wild-type enzyme | Escherichia coli |