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Literature summary extracted from

  • Paris, C.G.; Magasanik, B.
    Purification and properties of aromatic amino acid aminotransferase from Klebsiella aerogenes (1981), J. Bacteriol., 145, 266-271.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.57 Aminooxyacetate
-
 Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.57 0.02
-
 phenylpyruvate cosubstrate glutamate Klebsiella aerogenes
2.6.1.57 0.08
-
 L-phenylalanine cosubstrate 2-oxoglutarate Klebsiella aerogenes
2.6.1.57 0.12
-
 oxaloacetate cosubstrate tryptophan Klebsiella aerogenes
2.6.1.57 0.6
-
 L-tryptophan cosubstrate 2-oxoglutarate Klebsiella aerogenes
2.6.1.57 2.08
-
 2-oxoglutarate cosubstrate tryptophan Klebsiella aerogenes
2.6.1.57 70
-
 L-histidine cosubstrate 2-oxoglutarate Klebsiella aerogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.57 42000
-
 2 * 42000, SDS-PAGE Klebsiella aerogenes
2.6.1.57 90000
-
 gel filtration Klebsiella aerogenes

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.57 Klebsiella aerogenes
-
 aromatic aminotransferase I
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.57 protamine, ammonium sulfate, DEAE-Sephadex, ultrogel AcA44, hydroxylapatite, isoelectric focusing Klebsiella aerogenes

Storage Stability

EC Number Storage Stability Organism
2.6.1.57 4°C, glycerol, 10% stabilizes during storage Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.57 L-histidine + 2-oxoglutarate
-
 Klebsiella aerogenes 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate
-
 ?
2.6.1.57 L-phenylalanine + 2-oxoglutarate
-
 Klebsiella aerogenes phenylpyruvate + L-glutamate
-
 ?
2.6.1.57 L-tryptophan + 2-oxoglutarate
-
 Klebsiella aerogenes 3-indole-2-oxopropanoate + L-glutamate
-
 ?
2.6.1.57 L-tryptophan + oxaloacetate
-
 Klebsiella aerogenes 3-indole-2-oxopropanoate + L-aspartate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.57 dimer 2 * 42000, SDS-PAGE Klebsiella aerogenes

Synonyms

EC Number Synonyms Comment Organism
2.6.1.57 aromatic amino transferase I
-
 Klebsiella aerogenes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.57 55
-
 10 min, 35% loss of activity Klebsiella aerogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.57 8
-
 with tryptophan Klebsiella aerogenes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.57 6 9.6 approx. 50% of maximal activity at pH 6.0 and pH 9.6 Klebsiella aerogenes

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.57 pyridoxal 5'-phosphate a pyridoxal phosphate protein Klebsiella aerogenes