BRENDA - Enzyme Database

Isolation, characterization, and functional expression of kynurenine aminotransferase cDNA from the yellow fever mosquito, Aedes aegypti(1)

Fang, J.; Han, Q.; Li, J.; Insect Biochem. Mol. Biol. 32, 943-950 (2002)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.6.1.7
expression of full length enzyme and truncated version lacking the mitochondrial leader sequence in Sf9 insect cells
Aedes aegypti
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.7
mitochondrion
deduced amino acid sequence contains a mitochondrial leader sequence
Aedes aegypti
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.7
48000
-
2 * 48000, truncated recombinant enzyme, SDS-PAGE
Aedes aegypti
2.6.1.7
53000
-
x * 53000, SDS-PAGE
Aedes aegypti
2.6.1.7
53490
-
x * 53490, deduced from nucleotide sequence
Aedes aegypti
2.6.1.7
97000
-
truncated recombinant enzyme, gel filtration
Aedes aegypti
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.7
Aedes aegypti
Q95VY4
yellow fever mosquito
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.7
truncated recombinant enzyme, DEAE-Sepharose, native PAGE
Aedes aegypti
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-kynurenine + 2-oxoadipate
25% of activity with pyruvate
636638
Aedes aegypti
kynurenic acid + 2-aminoadipate + H2O
-
636638
Aedes aegypti
?
2.6.1.7
L-kynurenine + 2-oxoglutarate
31% of activity with pyruvate
636638
Aedes aegypti
kynurenic acid + L-glutamate + H2O
-
636638
Aedes aegypti
?
2.6.1.7
L-kynurenine + oxaloacetate
90% of activity with pyruvate
636638
Aedes aegypti
kynurenic acid + L-aspartate + H2O
-
636638
Aedes aegypti
?
2.6.1.7
L-kynurenine + pyruvate
best amino acceptor
636638
Aedes aegypti
kynurenic acid + L-alanine + H2O
-
636638
Aedes aegypti
?
Subunits
EC Number
Subunits
Commentary
Organism
2.6.1.7
?
x * 53000, SDS-PAGE; x * 53490, deduced from nucleotide sequence
Aedes aegypti
2.6.1.7
dimer
2 * 48000, truncated recombinant enzyme, SDS-PAGE
Aedes aegypti
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.7
60
-
recombinant enzyme
Aedes aegypti
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.7
8.5
-
optima at pH 8.5 and 10.0
Aedes aegypti
2.6.1.7
10
-
optima at pH 8.5 and 10.0
Aedes aegypti
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.7
expression of full length enzyme and truncated version lacking the mitochondrial leader sequence in Sf9 insect cells
Aedes aegypti
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.7
mitochondrion
deduced amino acid sequence contains a mitochondrial leader sequence
Aedes aegypti
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.7
48000
-
2 * 48000, truncated recombinant enzyme, SDS-PAGE
Aedes aegypti
2.6.1.7
53000
-
x * 53000, SDS-PAGE
Aedes aegypti
2.6.1.7
53490
-
x * 53490, deduced from nucleotide sequence
Aedes aegypti
2.6.1.7
97000
-
truncated recombinant enzyme, gel filtration
Aedes aegypti
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.7
truncated recombinant enzyme, DEAE-Sepharose, native PAGE
Aedes aegypti
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-kynurenine + 2-oxoadipate
25% of activity with pyruvate
636638
Aedes aegypti
kynurenic acid + 2-aminoadipate + H2O
-
636638
Aedes aegypti
?
2.6.1.7
L-kynurenine + 2-oxoglutarate
31% of activity with pyruvate
636638
Aedes aegypti
kynurenic acid + L-glutamate + H2O
-
636638
Aedes aegypti
?
2.6.1.7
L-kynurenine + oxaloacetate
90% of activity with pyruvate
636638
Aedes aegypti
kynurenic acid + L-aspartate + H2O
-
636638
Aedes aegypti
?
2.6.1.7
L-kynurenine + pyruvate
best amino acceptor
636638
Aedes aegypti
kynurenic acid + L-alanine + H2O
-
636638
Aedes aegypti
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.6.1.7
?
x * 53000, SDS-PAGE; x * 53490, deduced from nucleotide sequence
Aedes aegypti
2.6.1.7
dimer
2 * 48000, truncated recombinant enzyme, SDS-PAGE
Aedes aegypti
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.7
60
-
recombinant enzyme
Aedes aegypti
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.7
8.5
-
optima at pH 8.5 and 10.0
Aedes aegypti
2.6.1.7
10
-
optima at pH 8.5 and 10.0
Aedes aegypti