BRENDA - Enzyme Database

Characterization of rat brain kynurenine aminotransferases I and II

Guidetti, P.; Okuno, E.; Schwarcz, R.; J. Neurosci. Res. 50, 457-465 (1997)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.7
3-hydroxykynurenine
0.6 mM, 54% inhibition of KAT I, 88% inhibition of KAT II
Rattus norvegicus
2.6.1.7
alpha-aminoadipate
strong inhibition of KAT II
Rattus norvegicus
2.6.1.7
glutamine
strong inhibition of KAT I
Rattus norvegicus
2.6.1.7
L-phenylalanine
strong inhibition of KAT I
Rattus norvegicus
2.6.1.7
L-tryptophan
strong inhibition of KAT I
Rattus norvegicus
2.6.1.7
additional information
KAT I is not inhibited by quisqualate
Rattus norvegicus
2.6.1.7
quisqualate
0.52 mM, 50% inhibition of KAT II, 2 mM, 80% inhibition
Rattus norvegicus
2.6.1.7
S-dichlorovinyl-L-cysteine
2 mM, 90% inhibition of KAT I and KAT II
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.7
0.024
-
2-oxoglutarate
pH 9.5, 37°C, KAT I
Rattus norvegicus
2.6.1.7
0.053
-
pyruvate
pH 9.5, 37°C, KAT I
Rattus norvegicus
2.6.1.7
0.717
-
pyruvate
pH 7.0, 37°C, KAT II
Rattus norvegicus
2.6.1.7
0.879
-
2-oxoglutarate
pH 7.0, 37°C, KAT II
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.7
Rattus norvegicus
-
KAT I and KAT II
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.7
DEAE-Sepharose, ammonium sulfate, Sephadex G-25, partial purification of KAT I and KAT II
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.7
brain
KAT I and KAT II
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-kynurenine + 2-oxoglutarate
KAT II shows equal activity with 2-oxoglutarate and pyruvate, KAT I shows lower activity with 2-oxoglutarate than with pyruvate, ratio of KAT I to KAT II in normal brain is approx. 1/4
636632
Rattus norvegicus
kynurenic acid + L-glutamate + H2O
-
636632
Rattus norvegicus
-
2.6.1.7
L-kynurenine + pyruvate
-
636632
Rattus norvegicus
kynurenic acid + L-alanine + H2O
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.7
7
7.5
KAT II
Rattus norvegicus
2.6.1.7
9
9.5
KAT I
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.7
3-hydroxykynurenine
0.6 mM, 54% inhibition of KAT I, 88% inhibition of KAT II
Rattus norvegicus
2.6.1.7
alpha-aminoadipate
strong inhibition of KAT II
Rattus norvegicus
2.6.1.7
glutamine
strong inhibition of KAT I
Rattus norvegicus
2.6.1.7
L-phenylalanine
strong inhibition of KAT I
Rattus norvegicus
2.6.1.7
L-tryptophan
strong inhibition of KAT I
Rattus norvegicus
2.6.1.7
additional information
KAT I is not inhibited by quisqualate
Rattus norvegicus
2.6.1.7
quisqualate
0.52 mM, 50% inhibition of KAT II, 2 mM, 80% inhibition
Rattus norvegicus
2.6.1.7
S-dichlorovinyl-L-cysteine
2 mM, 90% inhibition of KAT I and KAT II
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.7
0.024
-
2-oxoglutarate
pH 9.5, 37°C, KAT I
Rattus norvegicus
2.6.1.7
0.053
-
pyruvate
pH 9.5, 37°C, KAT I
Rattus norvegicus
2.6.1.7
0.717
-
pyruvate
pH 7.0, 37°C, KAT II
Rattus norvegicus
2.6.1.7
0.879
-
2-oxoglutarate
pH 7.0, 37°C, KAT II
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.7
DEAE-Sepharose, ammonium sulfate, Sephadex G-25, partial purification of KAT I and KAT II
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.7
brain
KAT I and KAT II
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-kynurenine + 2-oxoglutarate
KAT II shows equal activity with 2-oxoglutarate and pyruvate, KAT I shows lower activity with 2-oxoglutarate than with pyruvate, ratio of KAT I to KAT II in normal brain is approx. 1/4
636632
Rattus norvegicus
kynurenic acid + L-glutamate + H2O
-
636632
Rattus norvegicus
-
2.6.1.7
L-kynurenine + pyruvate
-
636632
Rattus norvegicus
kynurenic acid + L-alanine + H2O
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.7
7
7.5
KAT II
Rattus norvegicus
2.6.1.7
9
9.5
KAT I
Rattus norvegicus