BRENDA - Enzyme Database

Identification of a mitochondrial form of kynurenine aminotransferase/glutamine transaminase K from rat brain

Malherbe, P.; Alberati-Giani, D.; Koehler, C.; Cesura, A.M.; FEBS Lett. 367, 141-144 (1995)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.6.1.7
transient expression in HEK-293 cells
Rattus norvegicus
2.6.1.64
cDNA clone encoding KAT/GTK, transfection of HEK-293 cells
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.7
0.096
-
2-oxoglutarate
pH 8.0, 37C, recombinant mitochondrial enzyme
Rattus norvegicus
2.6.1.7
1.63
-
pyruvate
pH 8.0, 37C, recombinant mitochondrial enzyme
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.7
mitochondrion
cDNA encodes for a kynurenine aminotransferase that is identical to a soluble form but carries an additional stretch of 32 amino acids resembling a mitochondrial import sequence
Rattus norvegicus
5739
-
2.6.1.64
cytosol
-
Rattus norvegicus
5829
-
2.6.1.64
mitochondrion
-
Rattus norvegicus
5739
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.7
Rattus norvegicus
Q08415
brain KAT I
-
2.6.1.64
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.7
brain
-
Rattus norvegicus
-
2.6.1.64
brain
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-kynurenine + 2-oxoglutarate
enzyme has both kynurenine aminotransferase and glutamine transaminase K activity
636626
Rattus norvegicus
kynurenic acid + L-glutamate + H2O
-
636626
Rattus norvegicus
?
2.6.1.7
L-kynurenine + pyruvate
-
636626
Rattus norvegicus
kynurenic acid + L-alanine + H2O
-
636626
Rattus norvegicus
?
2.6.1.64
additional information
cysteine S-conjugate beta-lyase activity, identical with kynurenine pyruvate aminotransferase
636626
Rattus norvegicus
?
-
-
-
-
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.7
transient expression in HEK-293 cells
Rattus norvegicus
2.6.1.64
cDNA clone encoding KAT/GTK, transfection of HEK-293 cells
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.7
0.096
-
2-oxoglutarate
pH 8.0, 37C, recombinant mitochondrial enzyme
Rattus norvegicus
2.6.1.7
1.63
-
pyruvate
pH 8.0, 37C, recombinant mitochondrial enzyme
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.7
mitochondrion
cDNA encodes for a kynurenine aminotransferase that is identical to a soluble form but carries an additional stretch of 32 amino acids resembling a mitochondrial import sequence
Rattus norvegicus
5739
-
2.6.1.64
cytosol
-
Rattus norvegicus
5829
-
2.6.1.64
mitochondrion
-
Rattus norvegicus
5739
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.7
brain
-
Rattus norvegicus
-
2.6.1.64
brain
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-kynurenine + 2-oxoglutarate
enzyme has both kynurenine aminotransferase and glutamine transaminase K activity
636626
Rattus norvegicus
kynurenic acid + L-glutamate + H2O
-
636626
Rattus norvegicus
?
2.6.1.7
L-kynurenine + pyruvate
-
636626
Rattus norvegicus
kynurenic acid + L-alanine + H2O
-
636626
Rattus norvegicus
?
2.6.1.64
additional information
cysteine S-conjugate beta-lyase activity, identical with kynurenine pyruvate aminotransferase
636626
Rattus norvegicus
?
-
-
-
-