BRENDA - Enzyme Database

Cloning and functional expression of a soluble form of kynurenine/alpha-aminoadipate aminotransferase from rat kidney

Buchli, R.; Alberati-Giani, D.; Malherbe, P.; Kohler, C.; Broger, C.; Cesura, A.M.; J. Biol. Chem. 270, 29330-29335 (1995)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
2.6.1.39
medicine
L-2-aminoadipate, the substrate for AadAT, is a well known astroglial-specific toxin, knowledge of the cerebral disposition of this compound is instrumental for the elucidation of its mechanism of toxicity and possible relevance in pathology
Rattus norvegicus
Cloned(Commentary)
EC Number
Commentary
Organism
2.6.1.7
transient expression in HEK-293 cells
Rattus norvegicus
2.6.1.39
kynurenine/alpha-aminoadipate aminotransferase cDNA cloned and expressed in HEK-293 cells
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.7
0.045
-
2-oxoglutarate
pH 8.0, 37°C, recombinant enzyme, cosubstrate L-kyrunenine
Rattus norvegicus
2.6.1.7
0.95
-
L-kynurenine
pH 8.0, 37°C, recombinant enzyme
Rattus norvegicus
2.6.1.7
1.36
-
L-3-hydroxykynurenine
pH 8.0, 37°C, recombinant enzyme
Rattus norvegicus
2.6.1.7
5.6
-
L-glutamate
pH 8.0, 37°C, alpha-aminoadipate aminotransferase activity
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.7
soluble
-
Rattus norvegicus
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.7
45000
-
2 * 45000, SDS-PAGE
Rattus norvegicus
2.6.1.7
90000
100000
gel filtration
Rattus norvegicus
2.6.1.39
45000
-
2 * 45000, SDS-PAGE
Rattus norvegicus
2.6.1.39
47789
-
2 * 47789, amino acid residues
Rattus norvegicus
2.6.1.39
100000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
Rattus norvegicus
lysine catabolic pathway
2-oxoadipate + L-glutamate
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.7
Rattus norvegicus
Q64602
-
-
2.6.1.39
Rattus norvegicus
Q64602
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.7
ammonium sulfate, DEAE-Sepharose, phenyl-Sepharose, Mono Q, Mono P
Rattus norvegicus
2.6.1.39
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.7
kidney
-
Rattus norvegicus
-
2.6.1.39
kidney
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.7
1.56
-
-
Rattus norvegicus
2.6.1.39
1.56
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-3-hydroxykynurenine + 2-oxoglutarate
-
636625
Rattus norvegicus
xanthurenic acid + L-glutamate
-
636625
Rattus norvegicus
-
2.6.1.7
L-kynurenine + 2-oxoadipate
91% of activity with 2-oxoglutarate
636625
Rattus norvegicus
kynurenic acid + 2-aminoadipate + H2O
-
636625
Rattus norvegicus
?
2.6.1.7
L-kynurenine + 2-oxobutyrate
28% of activity with 2-oxoglutarate
636625
Rattus norvegicus
kynurenic acid + 2-aminobutanoate + H2O
-
636625
Rattus norvegicus
?
2.6.1.7
L-kynurenine + 2-oxoglutarate
enzyme has both kynurenine aminotransferase and alpha-aminoadipate aminotransferase activity
636625
Rattus norvegicus
kynurenic acid + L-glutamate + H2O
-
636625
Rattus norvegicus
?
2.6.1.7
L-kynurenine + 4-methyl-2-oxovalerate
5.9% of activity with 2-oxoglutarate
636625
Rattus norvegicus
kynurenic acid + 2,4-dimethylvalerate + H2O
-
636625
Rattus norvegicus
?
2.6.1.7
L-kynurenine + phenylpyruvate
43% of activity with 2-oxoglutarate
636625
Rattus norvegicus
phenylalanine + L-glutamate + H2O
-
636625
Rattus norvegicus
?
2.6.1.7
L-kynurenine + pyruvate
3% of activity with 2-oxoglutarate
636625
Rattus norvegicus
kynurenic acid + L-alanine + H2O
-
636625
Rattus norvegicus
?
2.6.1.39
kynurenine + 2-oxoglutarate
-
636625
Rattus norvegicus
kynurenic acid + L-glutamate
-
-
-
ir
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
-
636625
Rattus norvegicus
2-oxoadipate + L-glutamate
-
-
-
r
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
lysine catabolic pathway
636625
Rattus norvegicus
2-oxoadipate + L-glutamate
-
-
-
r
2.6.1.39
additional information
2-aminoadipate aminotransferase and kynurenine aminotransferase, EC 2.6.1.7, activities are properties of a single protein
636625
Rattus norvegicus
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.6.1.7
dimer
2 * 45000, SDS-PAGE
Rattus norvegicus
2.6.1.39
dimer
2 * 45000, SDS-PAGE; 2 * 47789, amino acid residues
Rattus norvegicus
Application (protein specific)
EC Number
Application
Commentary
Organism
2.6.1.39
medicine
L-2-aminoadipate, the substrate for AadAT, is a well known astroglial-specific toxin, knowledge of the cerebral disposition of this compound is instrumental for the elucidation of its mechanism of toxicity and possible relevance in pathology
Rattus norvegicus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.7
transient expression in HEK-293 cells
Rattus norvegicus
2.6.1.39
kynurenine/alpha-aminoadipate aminotransferase cDNA cloned and expressed in HEK-293 cells
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.7
0.045
-
2-oxoglutarate
pH 8.0, 37°C, recombinant enzyme, cosubstrate L-kyrunenine
Rattus norvegicus
2.6.1.7
0.95
-
L-kynurenine
pH 8.0, 37°C, recombinant enzyme
Rattus norvegicus
2.6.1.7
1.36
-
L-3-hydroxykynurenine
pH 8.0, 37°C, recombinant enzyme
Rattus norvegicus
2.6.1.7
5.6
-
L-glutamate
pH 8.0, 37°C, alpha-aminoadipate aminotransferase activity
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.7
soluble
-
Rattus norvegicus
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.7
45000
-
2 * 45000, SDS-PAGE
Rattus norvegicus
2.6.1.7
90000
100000
gel filtration
Rattus norvegicus
2.6.1.39
45000
-
2 * 45000, SDS-PAGE
Rattus norvegicus
2.6.1.39
47789
-
2 * 47789, amino acid residues
Rattus norvegicus
2.6.1.39
100000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
Rattus norvegicus
lysine catabolic pathway
2-oxoadipate + L-glutamate
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.7
ammonium sulfate, DEAE-Sepharose, phenyl-Sepharose, Mono Q, Mono P
Rattus norvegicus
2.6.1.39
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.7
kidney
-
Rattus norvegicus
-
2.6.1.39
kidney
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.7
1.56
-
-
Rattus norvegicus
2.6.1.39
1.56
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-3-hydroxykynurenine + 2-oxoglutarate
-
636625
Rattus norvegicus
xanthurenic acid + L-glutamate
-
636625
Rattus norvegicus
-
2.6.1.7
L-kynurenine + 2-oxoadipate
91% of activity with 2-oxoglutarate
636625
Rattus norvegicus
kynurenic acid + 2-aminoadipate + H2O
-
636625
Rattus norvegicus
?
2.6.1.7
L-kynurenine + 2-oxobutyrate
28% of activity with 2-oxoglutarate
636625
Rattus norvegicus
kynurenic acid + 2-aminobutanoate + H2O
-
636625
Rattus norvegicus
?
2.6.1.7
L-kynurenine + 2-oxoglutarate
enzyme has both kynurenine aminotransferase and alpha-aminoadipate aminotransferase activity
636625
Rattus norvegicus
kynurenic acid + L-glutamate + H2O
-
636625
Rattus norvegicus
?
2.6.1.7
L-kynurenine + 4-methyl-2-oxovalerate
5.9% of activity with 2-oxoglutarate
636625
Rattus norvegicus
kynurenic acid + 2,4-dimethylvalerate + H2O
-
636625
Rattus norvegicus
?
2.6.1.7
L-kynurenine + phenylpyruvate
43% of activity with 2-oxoglutarate
636625
Rattus norvegicus
phenylalanine + L-glutamate + H2O
-
636625
Rattus norvegicus
?
2.6.1.7
L-kynurenine + pyruvate
3% of activity with 2-oxoglutarate
636625
Rattus norvegicus
kynurenic acid + L-alanine + H2O
-
636625
Rattus norvegicus
?
2.6.1.39
kynurenine + 2-oxoglutarate
-
636625
Rattus norvegicus
kynurenic acid + L-glutamate
-
-
-
ir
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
-
636625
Rattus norvegicus
2-oxoadipate + L-glutamate
-
-
-
r
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
lysine catabolic pathway
636625
Rattus norvegicus
2-oxoadipate + L-glutamate
-
-
-
r
2.6.1.39
additional information
2-aminoadipate aminotransferase and kynurenine aminotransferase, EC 2.6.1.7, activities are properties of a single protein
636625
Rattus norvegicus
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.6.1.7
dimer
2 * 45000, SDS-PAGE
Rattus norvegicus
2.6.1.39
dimer
2 * 45000, SDS-PAGE; 2 * 47789, amino acid residues
Rattus norvegicus