BRENDA - Enzyme Database

Purification, characterization and identification of rat liver mitochondrial kynurenine aminotransferase with alpha-aminoadipate aminotransferase

Takeuchi, F.; Otsuka, H.; Shibata, Y.; Biochim. Biophys. Acta 743, 323-330 (1983)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.7
2-Aminoadipate
-
Rattus norvegicus
2.6.1.7
2-oxoadipate
-
Rattus norvegicus
2.6.1.7
2-oxoglutarate
substrate inhibition
Rattus norvegicus
2.6.1.7
4-chloromercuribenzoate
-
Rattus norvegicus
2.6.1.7
adipate
strong inhibition
Rattus norvegicus
2.6.1.7
anthranilate
weak inhibition
Rattus norvegicus
2.6.1.7
Co2+
-
Rattus norvegicus
2.6.1.7
Cu2+
-
Rattus norvegicus
2.6.1.7
Fe3+
-
Rattus norvegicus
2.6.1.7
glutamate
weak inhibition
Rattus norvegicus
2.6.1.7
Hg2+
strong inhibition
Rattus norvegicus
2.6.1.7
Kynurenate
weak inhibition
Rattus norvegicus
2.6.1.7
L-kynurenine
substrate inhibition above 6 mM
Rattus norvegicus
2.6.1.7
additional information
not inhibited by glycerol, DTT, up to 10%, tryptophan, 3-hydroxyanthranilate, picolinate, NAD+, NADH, NADP+, NADPH, serotonin, tryptamine, 5-hydroxytryptophan, indole-3-acetate, organic acids of tricarboxylic acid cycle, glyoxylate, pyruvate, glutamine, Mg2+, Ca2+, Mn2+, monovalent cations; not inhibited by quinolinate
Rattus norvegicus
2.6.1.7
nicotinate
weak inhibition
Rattus norvegicus
2.6.1.7
xanthurenate
weak inhibition
Rattus norvegicus
2.6.1.7
Zn2+
-
Rattus norvegicus
2.6.1.39
alpha-aminoadipate
competitive inhibition against kynurenine or 3-hydroxykynurenine
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.7
0.01
-
2-oxoadipate
-
Rattus norvegicus
2.6.1.7
0.013
-
2-oxoglutarate
cosubstrate hydroxykynurenine
Rattus norvegicus
2.6.1.7
0.02
-
2-oxoglutarate
cosubstrate L-kynurenine
Rattus norvegicus
2.6.1.7
4.3
-
L-kynurenine
-
Rattus norvegicus
2.6.1.39
0.01
-
2-oxoadipate
pH 6.5, 37C
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.7
mitochondrial inner membrane
-
Rattus norvegicus
5743
-
2.6.1.7
mitochondrion
-
Rattus norvegicus
5739
-
2.6.1.39
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.7
49500
-
2 * 49500, SDS-PAGE
Rattus norvegicus
2.6.1.39
49500
-
2 * 49500, SDS-PAGE
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.7
Rattus norvegicus
-
male Wistar
-
2.6.1.39
Rattus norvegicus
-
Wistar
-
2.6.1.39
Rattus norvegicus Wistar
-
Wistar
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.7
-
Rattus norvegicus
2.6.1.39
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.7
liver
-
Rattus norvegicus
-
2.6.1.39
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.6.1.7
0.13
-
hydroxykynurenine
Rattus norvegicus
2.6.1.7
1.5
-
L-kynurenine
Rattus norvegicus
2.6.1.39
1.5
-
kynurenine aminotransferase activity
Rattus norvegicus
2.6.1.39
6.3
-
alpha-aminoadipate aminotransferase activity
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
2.6.1.7
-20C, in 10 mM phosphate buffer, pH 7, 50% glycerol, at least 2 months
Rattus norvegicus
2.6.1.39
-80C, stable for more than 1 month
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-3-hydroxykynurenine + 2-oxoglutarate
-
636623
Rattus norvegicus
xanthurenic acid + L-glutamate
-
636623
Rattus norvegicus
ir
2.6.1.7
L-kynurenine + 2-oxoglutarate
best substrates
636623
Rattus norvegicus
kynurenic acid + L-glutamate + H2O
-
636623
Rattus norvegicus
ir
2.6.1.7
L-kynurenine + 2-oxoglutarate
best substrates
636623
Rattus norvegicus Wistar
kynurenic acid + L-glutamate + H2O
-
636623
Rattus norvegicus Wistar
ir
2.6.1.7
L-kynurenine + 2-oxohexanedioate
-
636623
Rattus norvegicus
kynurenic acid + 2-aminohexanedioate + H2O
-
636623
Rattus norvegicus
?
2.6.1.7
L-kynurenine + 2-oxohexanedioate
-
636623
Rattus norvegicus Wistar
kynurenic acid + 2-aminohexanedioate + H2O
-
636623
Rattus norvegicus Wistar
?
2.6.1.7
L-kynurenine + glyoxylate
poor substrate
636623
Rattus norvegicus
kynurenic acid + glycine + H2O
-
636623
Rattus norvegicus
?
2.6.1.7
L-kynurenine + glyoxylate
poor substrate
636623
Rattus norvegicus Wistar
kynurenic acid + glycine + H2O
-
636623
Rattus norvegicus Wistar
?
2.6.1.7
L-kynurenine + oxaloacetate
less effective than 2-oxoglutarate
636623
Rattus norvegicus
kynurenic acid + L-aspartate + H2O
-
636623
Rattus norvegicus
?
2.6.1.7
L-kynurenine + oxaloacetate
less effective than 2-oxoglutarate
636623
Rattus norvegicus Wistar
kynurenic acid + L-aspartate + H2O
-
636623
Rattus norvegicus Wistar
?
2.6.1.7
L-kynurenine + pyruvate
less effective than 2-oxoglutarate
636623
Rattus norvegicus
kynurenic acid + L-alanine + H2O
-
636623
Rattus norvegicus
?
2.6.1.7
L-kynurenine + pyruvate
less effective than 2-oxoglutarate
636623
Rattus norvegicus Wistar
kynurenic acid + L-alanine + H2O
-
636623
Rattus norvegicus Wistar
?
2.6.1.39
additional information
2-aminoadipate aminotransferase and kynurenine aminotransferase, EC 2.6.1.7, activities are properties of a single protein
636623
Rattus norvegicus
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.6.1.7
dimer
2 * 49500, SDS-PAGE
Rattus norvegicus
2.6.1.39
dimer
2 * 49500, SDS-PAGE
Rattus norvegicus
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.6.1.7
37
-
assay at
Rattus norvegicus
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.6.1.7
50
-
in 20% glycerol, 0.1 mM DTT, 2 mM pyridoxal phosphate, 25 mM imidazole-HCl buffer, pH 7, 30 min stable
Rattus norvegicus
2.6.1.7
70
-
20 min, inactivation
Rattus norvegicus
2.6.1.39
50
70
both activities are not changed by heating at 50C for 30 min, activities show a parallel decrease with time at 60C and 70C, both are completely lost by incubation at 70C for 20 min
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.7
6.5
-
-
Rattus norvegicus
2.6.1.39
6.5
-
kynurenine aminotransferase activity
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.7
pyridoxal 5'-phosphate
-
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.7
pyridoxal 5'-phosphate
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.7
2-Aminoadipate
-
Rattus norvegicus
2.6.1.7
2-oxoadipate
-
Rattus norvegicus
2.6.1.7
2-oxoglutarate
substrate inhibition
Rattus norvegicus
2.6.1.7
4-chloromercuribenzoate
-
Rattus norvegicus
2.6.1.7
adipate
strong inhibition
Rattus norvegicus
2.6.1.7
anthranilate
weak inhibition
Rattus norvegicus
2.6.1.7
Co2+
-
Rattus norvegicus
2.6.1.7
Cu2+
-
Rattus norvegicus
2.6.1.7
Fe3+
-
Rattus norvegicus
2.6.1.7
glutamate
weak inhibition
Rattus norvegicus
2.6.1.7
Hg2+
strong inhibition
Rattus norvegicus
2.6.1.7
Kynurenate
weak inhibition
Rattus norvegicus
2.6.1.7
L-kynurenine
substrate inhibition above 6 mM
Rattus norvegicus
2.6.1.7
additional information
not inhibited by glycerol, DTT, up to 10%, tryptophan, 3-hydroxyanthranilate, picolinate, NAD+, NADH, NADP+, NADPH, serotonin, tryptamine, 5-hydroxytryptophan, indole-3-acetate, organic acids of tricarboxylic acid cycle, glyoxylate, pyruvate, glutamine, Mg2+, Ca2+, Mn2+, monovalent cations; not inhibited by quinolinate
Rattus norvegicus
2.6.1.7
nicotinate
weak inhibition
Rattus norvegicus
2.6.1.7
xanthurenate
weak inhibition
Rattus norvegicus
2.6.1.7
Zn2+
-
Rattus norvegicus
2.6.1.39
alpha-aminoadipate
competitive inhibition against kynurenine or 3-hydroxykynurenine
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.7
0.01
-
2-oxoadipate
-
Rattus norvegicus
2.6.1.7
0.013
-
2-oxoglutarate
cosubstrate hydroxykynurenine
Rattus norvegicus
2.6.1.7
0.02
-
2-oxoglutarate
cosubstrate L-kynurenine
Rattus norvegicus
2.6.1.7
4.3
-
L-kynurenine
-
Rattus norvegicus
2.6.1.39
0.01
-
2-oxoadipate
pH 6.5, 37C
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.7
mitochondrial inner membrane
-
Rattus norvegicus
5743
-
2.6.1.7
mitochondrion
-
Rattus norvegicus
5739
-
2.6.1.39
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.7
49500
-
2 * 49500, SDS-PAGE
Rattus norvegicus
2.6.1.39
49500
-
2 * 49500, SDS-PAGE
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.7
-
Rattus norvegicus
2.6.1.39
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.7
liver
-
Rattus norvegicus
-
2.6.1.39
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.6.1.7
0.13
-
hydroxykynurenine
Rattus norvegicus
2.6.1.7
1.5
-
L-kynurenine
Rattus norvegicus
2.6.1.39
1.5
-
kynurenine aminotransferase activity
Rattus norvegicus
2.6.1.39
6.3
-
alpha-aminoadipate aminotransferase activity
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.6.1.7
-20C, in 10 mM phosphate buffer, pH 7, 50% glycerol, at least 2 months
Rattus norvegicus
2.6.1.39
-80C, stable for more than 1 month
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-3-hydroxykynurenine + 2-oxoglutarate
-
636623
Rattus norvegicus
xanthurenic acid + L-glutamate
-
636623
Rattus norvegicus
ir
2.6.1.7
L-kynurenine + 2-oxoglutarate
best substrates
636623
Rattus norvegicus
kynurenic acid + L-glutamate + H2O
-
636623
Rattus norvegicus
ir
2.6.1.7
L-kynurenine + 2-oxoglutarate
best substrates
636623
Rattus norvegicus Wistar
kynurenic acid + L-glutamate + H2O
-
636623
Rattus norvegicus Wistar
ir
2.6.1.7
L-kynurenine + 2-oxohexanedioate
-
636623
Rattus norvegicus
kynurenic acid + 2-aminohexanedioate + H2O
-
636623
Rattus norvegicus
?
2.6.1.7
L-kynurenine + 2-oxohexanedioate
-
636623
Rattus norvegicus Wistar
kynurenic acid + 2-aminohexanedioate + H2O
-
636623
Rattus norvegicus Wistar
?
2.6.1.7
L-kynurenine + glyoxylate
poor substrate
636623
Rattus norvegicus
kynurenic acid + glycine + H2O
-
636623
Rattus norvegicus
?
2.6.1.7
L-kynurenine + glyoxylate
poor substrate
636623
Rattus norvegicus Wistar
kynurenic acid + glycine + H2O
-
636623
Rattus norvegicus Wistar
?
2.6.1.7
L-kynurenine + oxaloacetate
less effective than 2-oxoglutarate
636623
Rattus norvegicus
kynurenic acid + L-aspartate + H2O
-
636623
Rattus norvegicus
?
2.6.1.7
L-kynurenine + oxaloacetate
less effective than 2-oxoglutarate
636623
Rattus norvegicus Wistar
kynurenic acid + L-aspartate + H2O
-
636623
Rattus norvegicus Wistar
?
2.6.1.7
L-kynurenine + pyruvate
less effective than 2-oxoglutarate
636623
Rattus norvegicus
kynurenic acid + L-alanine + H2O
-
636623
Rattus norvegicus
?
2.6.1.7
L-kynurenine + pyruvate
less effective than 2-oxoglutarate
636623
Rattus norvegicus Wistar
kynurenic acid + L-alanine + H2O
-
636623
Rattus norvegicus Wistar
?
2.6.1.39
additional information
2-aminoadipate aminotransferase and kynurenine aminotransferase, EC 2.6.1.7, activities are properties of a single protein
636623
Rattus norvegicus
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.6.1.7
dimer
2 * 49500, SDS-PAGE
Rattus norvegicus
2.6.1.39
dimer
2 * 49500, SDS-PAGE
Rattus norvegicus
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.6.1.7
37
-
assay at
Rattus norvegicus
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.6.1.7
50
-
in 20% glycerol, 0.1 mM DTT, 2 mM pyridoxal phosphate, 25 mM imidazole-HCl buffer, pH 7, 30 min stable
Rattus norvegicus
2.6.1.7
70
-
20 min, inactivation
Rattus norvegicus
2.6.1.39
50
70
both activities are not changed by heating at 50C for 30 min, activities show a parallel decrease with time at 60C and 70C, both are completely lost by incubation at 70C for 20 min
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.7
6.5
-
-
Rattus norvegicus
2.6.1.39
6.5
-
kynurenine aminotransferase activity
Rattus norvegicus