Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Takeuchi, F.; Otsuka, H.; Shibata, Y.
    Purification, characterization and identification of rat liver mitochondrial kynurenine aminotransferase with alpha-aminoadipate aminotransferase (1983), Biochim. Biophys. Acta, 743, 323-330.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.7 2-Aminoadipate
-
 Rattus norvegicus
2.6.1.7 2-oxoadipate
-
 Rattus norvegicus
2.6.1.7 2-oxoglutarate substrate inhibition Rattus norvegicus
2.6.1.7 4-chloromercuribenzoate
-
 Rattus norvegicus
2.6.1.7 adipate strong inhibition Rattus norvegicus
2.6.1.7 anthranilate weak inhibition Rattus norvegicus
2.6.1.7 Co2+
-
 Rattus norvegicus
2.6.1.7 Cu2+
-
 Rattus norvegicus
2.6.1.7 Fe3+
-
 Rattus norvegicus
2.6.1.7 glutamate weak inhibition Rattus norvegicus
2.6.1.7 Hg2+ strong inhibition Rattus norvegicus
2.6.1.7 Kynurenate weak inhibition Rattus norvegicus
2.6.1.7 L-kynurenine substrate inhibition above 6 mM Rattus norvegicus
2.6.1.7 additional information not inhibited by glycerol, DTT, up to 10%, tryptophan, 3-hydroxyanthranilate, picolinate, NAD+, NADH, NADP+, NADPH, serotonin, tryptamine, 5-hydroxytryptophan, indole-3-acetate, organic acids of tricarboxylic acid cycle, glyoxylate, pyruvate, glutamine, Mg2+, Ca2+, Mn2+, monovalent cations; not inhibited by quinolinate Rattus norvegicus
2.6.1.7 nicotinate weak inhibition Rattus norvegicus
2.6.1.7 xanthurenate weak inhibition Rattus norvegicus
2.6.1.7 Zn2+
-
 Rattus norvegicus
2.6.1.39 alpha-aminoadipate competitive inhibition against kynurenine or 3-hydroxykynurenine Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.7 0.01
-
 2-oxoadipate
-
 Rattus norvegicus
2.6.1.7 0.013
-
 2-oxoglutarate cosubstrate hydroxykynurenine Rattus norvegicus
2.6.1.7 0.02
-
 2-oxoglutarate cosubstrate L-kynurenine Rattus norvegicus
2.6.1.7 4.3
-
 L-kynurenine
-
 Rattus norvegicus
2.6.1.39 0.01
-
 2-oxoadipate pH 6.5, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.7 mitochondrial inner membrane
-
 Rattus norvegicus 5743
-
2.6.1.7 mitochondrion
-
 Rattus norvegicus 5739
-
2.6.1.39 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.7 49500
-
 2 * 49500, SDS-PAGE Rattus norvegicus
2.6.1.39 49500
-
 2 * 49500, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.7 Rattus norvegicus
-
 male Wistar
-
2.6.1.39 Rattus norvegicus
-
 Wistar
-
2.6.1.39 Rattus norvegicus Wistar
-
 Wistar
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.7
-
 Rattus norvegicus
2.6.1.39
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.7 liver
-
 Rattus norvegicus
-
2.6.1.39 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.7 0.13
-
 hydroxykynurenine Rattus norvegicus
2.6.1.7 1.5
-
 L-kynurenine Rattus norvegicus
2.6.1.39 1.5
-
 kynurenine aminotransferase activity Rattus norvegicus
2.6.1.39 6.3
-
 alpha-aminoadipate aminotransferase activity Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.6.1.7 -20°C, in 10 mM phosphate buffer, pH 7, 50% glycerol, at least 2 months Rattus norvegicus
2.6.1.39 -80°C, stable for more than 1 month Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.7 L-3-hydroxykynurenine + 2-oxoglutarate
-
 Rattus norvegicus xanthurenic acid + L-glutamate
-
 ir
2.6.1.7 L-kynurenine + 2-oxoglutarate best substrates Rattus norvegicus kynurenic acid + L-glutamate + H2O
-
 ir
2.6.1.7 L-kynurenine + 2-oxoglutarate best substrates Rattus norvegicus Wistar kynurenic acid + L-glutamate + H2O
-
 ir
2.6.1.7 L-kynurenine + 2-oxohexanedioate
-
 Rattus norvegicus kynurenic acid + 2-aminohexanedioate + H2O
-
 ?
2.6.1.7 L-kynurenine + 2-oxohexanedioate
-
 Rattus norvegicus Wistar kynurenic acid + 2-aminohexanedioate + H2O
-
 ?
2.6.1.7 L-kynurenine + glyoxylate poor substrate Rattus norvegicus kynurenic acid + glycine + H2O
-
 ?
2.6.1.7 L-kynurenine + glyoxylate poor substrate Rattus norvegicus Wistar kynurenic acid + glycine + H2O
-
 ?
2.6.1.7 L-kynurenine + oxaloacetate less effective than 2-oxoglutarate Rattus norvegicus kynurenic acid + L-aspartate + H2O
-
 ?
2.6.1.7 L-kynurenine + oxaloacetate less effective than 2-oxoglutarate Rattus norvegicus Wistar kynurenic acid + L-aspartate + H2O
-
 ?
2.6.1.7 L-kynurenine + pyruvate less effective than 2-oxoglutarate Rattus norvegicus kynurenic acid + L-alanine + H2O
-
 ?
2.6.1.7 L-kynurenine + pyruvate less effective than 2-oxoglutarate Rattus norvegicus Wistar kynurenic acid + L-alanine + H2O
-
 ?
2.6.1.39 additional information 2-aminoadipate aminotransferase and kynurenine aminotransferase, EC 2.6.1.7, activities are properties of a single protein Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.7 dimer 2 * 49500, SDS-PAGE Rattus norvegicus
2.6.1.39 dimer 2 * 49500, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.6.1.7 More enzyme from mitochondria may be identical with EC 2.6.1.39 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.7 37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.7 50
-
 in 20% glycerol, 0.1 mM DTT, 2 mM pyridoxal phosphate, 25 mM imidazole-HCl buffer, pH 7, 30 min stable Rattus norvegicus
2.6.1.7 70
-
 20 min, inactivation Rattus norvegicus
2.6.1.39 50 70 both activities are not changed by heating at 50°C for 30 min, activities show a parallel decrease with time at 60°C and 70°C, both are completely lost by incubation at 70°C for 20 min Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.7 6.5
-
-
 Rattus norvegicus
2.6.1.39 6.5
-
 kynurenine aminotransferase activity Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.7 pyridoxal 5'-phosphate
-
 Rattus norvegicus