BRENDA - Enzyme Database

Purification and properties of kynurenine aminotransferase from rat kidney

Mawal, M.R.; Mukhopadhyay, A.; Deshmukh, D.R.; Biochem. J. 279, 595-599 (1991)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.7
adipate
-
Rattus norvegicus
2.6.1.7
azelaic acid
-
Rattus norvegicus
2.6.1.7
Decanoic acid
-
Rattus norvegicus
2.6.1.7
Diethylglutaric acid
-
Rattus norvegicus
2.6.1.7
dimethylglutaric acid
-
Rattus norvegicus
2.6.1.7
additional information
not inhibited by 2-aminoadipate; not inhibited by oxaloacetic acid, 3-methylglutaric acid
Rattus norvegicus
2.6.1.7
pimelate
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.7
0.02
-
2-oxoglutarate
cosubstrate L-kynurenine
Rattus norvegicus
2.6.1.7
4
-
L-kynurenine
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.7
soluble
-
Rattus norvegicus
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.7
additional information
-
amino acid composition
Rattus norvegicus
2.6.1.7
44000
-
2 * 44000, SDS-PAGE
Rattus norvegicus
2.6.1.7
91000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
Rattus norvegicus
lysine catabolic pathway
2-oxoadipate + L-glutamate
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.7
Rattus norvegicus
-
-
-
2.6.1.39
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.7
-
Rattus norvegicus
2.6.1.39
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.7
kidney
-
Rattus norvegicus
-
2.6.1.39
kidney
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.7
4.22
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-3-hydroxykynurenine + 2-oxoglutarate
-
636621
Rattus norvegicus
xanthurenic acid + L-glutamate
-
636621
Rattus norvegicus
-
2.6.1.7
L-histidine + 2-oxoglutarate
-
636621
Rattus norvegicus
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate
-
636621
Rattus norvegicus
?
2.6.1.7
L-kynurenine + 2-oxoglutarate
best substrates
636621
Rattus norvegicus
kynurenic acid + L-glutamate + H2O
-
636621
Rattus norvegicus
-
2.6.1.7
L-kynurenine + pyruvate
less effective than 2-oxoglutarate
636621
Rattus norvegicus
kynurenic acid + L-alanine + H2O
-
636621
Rattus norvegicus
?
2.6.1.7
L-tryptophan + 2-oxoglutarate
poor substrate
636621
Rattus norvegicus
3-indole-2-oxopropanoate + L-glutamate
-
636621
Rattus norvegicus
?
2.6.1.7
L-tyrosine + 2-oxoglutarate
-
636621
Rattus norvegicus
3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
636621
Rattus norvegicus
?
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
-
636621
Rattus norvegicus
2-oxoadipate + L-glutamate
-
-
-
r
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
lysine catabolic pathway
636621
Rattus norvegicus
2-oxoadipate + L-glutamate
-
-
-
r
2.6.1.39
additional information
KAT and AadAT activities are associated with 2 different proteins
636621
Rattus norvegicus
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.6.1.7
dimer
2 * 44000, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.7
37
-
assay at
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.7
additional information
-
pI: 5.9
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.7
adipate
-
Rattus norvegicus
2.6.1.7
azelaic acid
-
Rattus norvegicus
2.6.1.7
Decanoic acid
-
Rattus norvegicus
2.6.1.7
Diethylglutaric acid
-
Rattus norvegicus
2.6.1.7
dimethylglutaric acid
-
Rattus norvegicus
2.6.1.7
additional information
not inhibited by 2-aminoadipate; not inhibited by oxaloacetic acid, 3-methylglutaric acid
Rattus norvegicus
2.6.1.7
pimelate
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.7
0.02
-
2-oxoglutarate
cosubstrate L-kynurenine
Rattus norvegicus
2.6.1.7
4
-
L-kynurenine
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.7
soluble
-
Rattus norvegicus
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.7
additional information
-
amino acid composition
Rattus norvegicus
2.6.1.7
44000
-
2 * 44000, SDS-PAGE
Rattus norvegicus
2.6.1.7
91000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
Rattus norvegicus
lysine catabolic pathway
2-oxoadipate + L-glutamate
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.7
-
Rattus norvegicus
2.6.1.39
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.7
kidney
-
Rattus norvegicus
-
2.6.1.39
kidney
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.7
4.22
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-3-hydroxykynurenine + 2-oxoglutarate
-
636621
Rattus norvegicus
xanthurenic acid + L-glutamate
-
636621
Rattus norvegicus
-
2.6.1.7
L-histidine + 2-oxoglutarate
-
636621
Rattus norvegicus
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate
-
636621
Rattus norvegicus
?
2.6.1.7
L-kynurenine + 2-oxoglutarate
best substrates
636621
Rattus norvegicus
kynurenic acid + L-glutamate + H2O
-
636621
Rattus norvegicus
-
2.6.1.7
L-kynurenine + pyruvate
less effective than 2-oxoglutarate
636621
Rattus norvegicus
kynurenic acid + L-alanine + H2O
-
636621
Rattus norvegicus
?
2.6.1.7
L-tryptophan + 2-oxoglutarate
poor substrate
636621
Rattus norvegicus
3-indole-2-oxopropanoate + L-glutamate
-
636621
Rattus norvegicus
?
2.6.1.7
L-tyrosine + 2-oxoglutarate
-
636621
Rattus norvegicus
3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
636621
Rattus norvegicus
?
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
-
636621
Rattus norvegicus
2-oxoadipate + L-glutamate
-
-
-
r
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
lysine catabolic pathway
636621
Rattus norvegicus
2-oxoadipate + L-glutamate
-
-
-
r
2.6.1.39
additional information
KAT and AadAT activities are associated with 2 different proteins
636621
Rattus norvegicus
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.6.1.7
dimer
2 * 44000, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.7
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.7
additional information
-
pI: 5.9
Rattus norvegicus