BRENDA - Enzyme Database

Kynurenine aminotransferase from kidney supernatant

Hartline, R.A.; Methods Enzymol. 113, 664-672 (1985)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.7
mitochondrion
-
Rattus norvegicus
5739
-
2.6.1.7
soluble
-
Rattus norvegicus
-
-
2.6.1.39
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.7
45000
-
2 * 45000, SDS-PAGE in the absence of 2-mercaptoethanol
Rattus norvegicus
2.6.1.7
85000
-
-
Rattus norvegicus
2.6.1.39
45000
-
2 * 45000, SDS-PAGE
Rattus norvegicus
2.6.1.39
85000
-
-
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
Rattus norvegicus
-
2-oxoadipate + L-glutamate
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.7
Rattus norvegicus
-
adult albino; male
-
2.6.1.39
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.7
-
Rattus norvegicus
2.6.1.39
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.7
kidney
-
Rattus norvegicus
-
2.6.1.7
liver
-
Rattus norvegicus
-
2.6.1.39
kidney
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.7
2.65
-
-
Rattus norvegicus
2.6.1.39
2.65
-
kynurenine aminotransferase activity
Rattus norvegicus
2.6.1.39
26.1
-
alpha-aminoadipate aminotransferase activity
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-kynurenine + 2-oxoglutarate
broad specificity
636620
Rattus norvegicus
kynurenic acid + L-glutamate + H2O
-
636620
Rattus norvegicus
-
2.6.1.39
3,5-di-iodotyrosine + 2-oxoglutarate
-
636620
Rattus norvegicus
3,5-diiodophenylpyruvate + L-glutamate
-
-
-
r
2.6.1.39
DL-2-aminopimelic acid + 2-oxoglutarate
-
636620
Rattus norvegicus
2-oxoadipate + L-glutamate
-
-
-
r
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
-
636620
Rattus norvegicus
2-oxoadipate + L-glutamate
-
-
-
r
2.6.1.39
L-norleucine + 2-oxoglutarate
-
636620
Rattus norvegicus
2-oxohexanoate + L-glutamate
-
-
-
r
2.6.1.39
additional information
less than 2% activity with L-aspartate, L-alanine, L-alpha-aminobutyrate, glycine, L-valine, L-leucine, L-isoleucine, L-norvaline, L-epsilon-aminocaproate, L-threonine, L-serine, L-homoserine, L-cysteine, L-methionine, L-lysine, L-arginine, L-citrulline, L-ornithine, L-histidine, L-tyrosine, L-tryptophan, L-phenylalanine, L-proline and L-glutamate
636620
Rattus norvegicus
?
-
-
-
-
2.6.1.39
additional information
2-aminoadipate aminotransferase and kynurenine aminotransferase, EC 2.6.1.7, activities are properties of a single protein
636620
Rattus norvegicus
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.6.1.7
dimer
2 * 45000, SDS-PAGE in the absence of 2-mercaptoethanol
Rattus norvegicus
2.6.1.39
dimer
2 * 45000, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.7
37
-
assay at
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.7
additional information
-
pI: 6.56
Rattus norvegicus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.6.1.39
6.5
7.5
apoenzyme is most stable in this range during purification
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.7
mitochondrion
-
Rattus norvegicus
5739
-
2.6.1.7
soluble
-
Rattus norvegicus
-
-
2.6.1.39
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.7
45000
-
2 * 45000, SDS-PAGE in the absence of 2-mercaptoethanol
Rattus norvegicus
2.6.1.7
85000
-
-
Rattus norvegicus
2.6.1.39
45000
-
2 * 45000, SDS-PAGE
Rattus norvegicus
2.6.1.39
85000
-
-
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
Rattus norvegicus
-
2-oxoadipate + L-glutamate
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.7
-
Rattus norvegicus
2.6.1.39
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.7
kidney
-
Rattus norvegicus
-
2.6.1.7
liver
-
Rattus norvegicus
-
2.6.1.39
kidney
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.7
2.65
-
-
Rattus norvegicus
2.6.1.39
2.65
-
kynurenine aminotransferase activity
Rattus norvegicus
2.6.1.39
26.1
-
alpha-aminoadipate aminotransferase activity
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-kynurenine + 2-oxoglutarate
broad specificity
636620
Rattus norvegicus
kynurenic acid + L-glutamate + H2O
-
636620
Rattus norvegicus
-
2.6.1.39
3,5-di-iodotyrosine + 2-oxoglutarate
-
636620
Rattus norvegicus
3,5-diiodophenylpyruvate + L-glutamate
-
-
-
r
2.6.1.39
DL-2-aminopimelic acid + 2-oxoglutarate
-
636620
Rattus norvegicus
2-oxoadipate + L-glutamate
-
-
-
r
2.6.1.39
L-2-aminoadipate + 2-oxoglutarate
-
636620
Rattus norvegicus
2-oxoadipate + L-glutamate
-
-
-
r
2.6.1.39
L-norleucine + 2-oxoglutarate
-
636620
Rattus norvegicus
2-oxohexanoate + L-glutamate
-
-
-
r
2.6.1.39
additional information
less than 2% activity with L-aspartate, L-alanine, L-alpha-aminobutyrate, glycine, L-valine, L-leucine, L-isoleucine, L-norvaline, L-epsilon-aminocaproate, L-threonine, L-serine, L-homoserine, L-cysteine, L-methionine, L-lysine, L-arginine, L-citrulline, L-ornithine, L-histidine, L-tyrosine, L-tryptophan, L-phenylalanine, L-proline and L-glutamate
636620
Rattus norvegicus
?
-
-
-
-
2.6.1.39
additional information
2-aminoadipate aminotransferase and kynurenine aminotransferase, EC 2.6.1.7, activities are properties of a single protein
636620
Rattus norvegicus
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.6.1.7
dimer
2 * 45000, SDS-PAGE in the absence of 2-mercaptoethanol
Rattus norvegicus
2.6.1.39
dimer
2 * 45000, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.7
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.7
additional information
-
pI: 6.56
Rattus norvegicus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.6.1.39
6.5
7.5
apoenzyme is most stable in this range during purification
Rattus norvegicus