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Literature summary extracted from

  • Hartline, R.A.
    Kynurenine aminotransferase from kidney supernatant (1985), Methods Enzymol., 113, 664-672.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.7 mitochondrion
-
 Rattus norvegicus 5739
-
2.6.1.7 soluble
-
 Rattus norvegicus
-
-
2.6.1.39 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.7 45000
-
 2 * 45000, SDS-PAGE in the absence of 2-mercaptoethanol Rattus norvegicus
2.6.1.7 85000
-
-
 Rattus norvegicus
2.6.1.39 45000
-
 2 * 45000, SDS-PAGE Rattus norvegicus
2.6.1.39 85000
-
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.39 L-2-aminoadipate + 2-oxoglutarate Rattus norvegicus
-
 2-oxoadipate + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.7 Rattus norvegicus
-
 male
-
2.6.1.7 Rattus norvegicus
-
 adult albino
-
2.6.1.39 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.7
-
 Rattus norvegicus
2.6.1.39
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.7 kidney
-
 Rattus norvegicus
-
2.6.1.7 liver
-
 Rattus norvegicus
-
2.6.1.39 kidney
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.7 2.65
-
-
 Rattus norvegicus
2.6.1.39 2.65
-
 kynurenine aminotransferase activity Rattus norvegicus
2.6.1.39 26.1
-
 alpha-aminoadipate aminotransferase activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.7 L-kynurenine + 2-oxoglutarate broad specificity Rattus norvegicus kynurenic acid + L-glutamate + H2O
-
 ?
2.6.1.39 3,5-di-iodotyrosine + 2-oxoglutarate
-
 Rattus norvegicus 3,5-diiodophenylpyruvate + L-glutamate
-
 r
2.6.1.39 DL-2-aminopimelic acid + 2-oxoglutarate
-
 Rattus norvegicus 2-oxoadipate + L-glutamate
-
 r
2.6.1.39 L-2-aminoadipate + 2-oxoglutarate
-
 Rattus norvegicus 2-oxoadipate + L-glutamate
-
 r
2.6.1.39 L-norleucine + 2-oxoglutarate
-
 Rattus norvegicus 2-oxohexanoate + L-glutamate
-
 r
2.6.1.39 additional information less than 2% activity with L-aspartate, L-alanine, L-alpha-aminobutyrate, glycine, L-valine, L-leucine, L-isoleucine, L-norvaline, L-epsilon-aminocaproate, L-threonine, L-serine, L-homoserine, L-cysteine, L-methionine, L-lysine, L-arginine, L-citrulline, L-ornithine, L-histidine, L-tyrosine, L-tryptophan, L-phenylalanine, L-proline and L-glutamate Rattus norvegicus ?
-
 ?
2.6.1.39 additional information 2-aminoadipate aminotransferase and kynurenine aminotransferase, EC 2.6.1.7, activities are properties of a single protein Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.7 dimer 2 * 45000, SDS-PAGE in the absence of 2-mercaptoethanol Rattus norvegicus
2.6.1.39 dimer 2 * 45000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.6.1.39 halogenated tyrosine aminotransferase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.7 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.7 additional information
-
 pI: 6.56 Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.6.1.39 6.5 7.5 apoenzyme is most stable in this range during purification Rattus norvegicus