BRENDA - Enzyme Database

Deamination and gamma-addition reactions of vinylglycine catalyzed by yeast kynurenine aminotransferase, and suicidal inactivation of the enzyme during its processing

Asada, Y.; Tanizawa, K.; Yonaha, K.; Soda, K.; Agric. Biol. Chem. 52, 2873-2878 (1988)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.7
2.7
-
L-Vinylglycine
pH 8.0. 25°C
Hansenula schneggii
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.7
Hansenula schneggii
-
yeast
-
Reaction
EC Number
Reaction
Commentary
Organism
2.6.1.7
L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate
mechanism
Hansenula schneggii
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.7
0.17
-
-
Hansenula schneggii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636615
Hansenula schneggii
kynurenic acid + L-glutamate + H2O
-
636615
Hansenula schneggii
-
2.6.1.7
L-vinylglycine
deamination at 1% the rate of kynurenine, suicide substrate
636615
Hansenula schneggii
2-oxobutyrate + NH3
-
636615
Hansenula schneggii
?
2.6.1.7
L-vinylglycine + 1-propanethiol
gamma-addition reaction in the presence of alkanethiols
636615
Hansenula schneggii
S-n-propylhomocysteine
-
636615
Hansenula schneggii
?
2.6.1.7
L-vinylglycine + 2-oxobutyrate
-
636615
Hansenula schneggii
2-aminobutyrate + 2-oxo-3-butenoate
-
636615
Hansenula schneggii
?
2.6.1.7
L-vinylglycine + ethanethiol
gamma-addition reaction in the presence of alkanethiols
636615
Hansenula schneggii
ethionine
i.e. S-ethylhomocysteine
636615
Hansenula schneggii
?
2.6.1.7
L-vinylglycine + methanethiol
gamma-addition reaction in the presence of alkanethiols
636615
Hansenula schneggii
methionine
-
636615
Hansenula schneggii
?
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.7
2.7
-
L-Vinylglycine
pH 8.0. 25°C
Hansenula schneggii
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.7
0.17
-
-
Hansenula schneggii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636615
Hansenula schneggii
kynurenic acid + L-glutamate + H2O
-
636615
Hansenula schneggii
-
2.6.1.7
L-vinylglycine
deamination at 1% the rate of kynurenine, suicide substrate
636615
Hansenula schneggii
2-oxobutyrate + NH3
-
636615
Hansenula schneggii
?
2.6.1.7
L-vinylglycine + 1-propanethiol
gamma-addition reaction in the presence of alkanethiols
636615
Hansenula schneggii
S-n-propylhomocysteine
-
636615
Hansenula schneggii
?
2.6.1.7
L-vinylglycine + 2-oxobutyrate
-
636615
Hansenula schneggii
2-aminobutyrate + 2-oxo-3-butenoate
-
636615
Hansenula schneggii
?
2.6.1.7
L-vinylglycine + ethanethiol
gamma-addition reaction in the presence of alkanethiols
636615
Hansenula schneggii
ethionine
i.e. S-ethylhomocysteine
636615
Hansenula schneggii
?
2.6.1.7
L-vinylglycine + methanethiol
gamma-addition reaction in the presence of alkanethiols
636615
Hansenula schneggii
methionine
-
636615
Hansenula schneggii
?