BRENDA - Enzyme Database

L-Kynurenine transaminase from Hansenula schneggii

Tanizawa, K.; Asada, Y.; Soda, K.; Methods Enzymol. 113, 90-95 (1985)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
2.6.1.7
-
Hansenula schneggii
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.7
4-chloromercuribenzoate
-
Hansenula schneggii
2.6.1.7
Dicarboxylic acids
-
Hansenula schneggii
2.6.1.7
Hg2+
-
Hansenula schneggii
2.6.1.7
iodoacetate
-
Hansenula schneggii
2.6.1.7
additional information
not inhibited by EDTA, 2,2'-dipyridyl
Hansenula schneggii
2.6.1.7
N-ethylmaleimide
-
Hansenula schneggii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.7
1
-
2-oxoisocaproate
-
Hansenula schneggii
2.6.1.7
1.1
-
2-oxoglutarate
-
Hansenula schneggii
2.6.1.7
1.2
-
beta-phenylpyruvate
-
Hansenula schneggii
2.6.1.7
2.6
-
L-tryptophan
-
Hansenula schneggii
2.6.1.7
5
-
L-leucine
-
Hansenula schneggii
2.6.1.7
5
-
L-methionine
-
Hansenula schneggii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.7
98000
-
sedimentation equilibrium
Hansenula schneggii
2.6.1.7
102000
-
native PAGE
Hansenula schneggii
2.6.1.7
106000
-
gel filtration
Hansenula schneggii
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.7
Cutaneotrichosporon cutaneum
-
low activity
-
2.6.1.7
Debaryomyces hansenii
-
yeast
-
2.6.1.7
Diutina rugosa
-
low activity
-
2.6.1.7
Hansenula schneggii
-
yeast
-
2.6.1.7
Kluyveromyces marxianus
-
-
-
2.6.1.7
Naganishia albida
-
low activity
-
2.6.1.7
Saccharomyces cerevisiae
-
-
-
2.6.1.7
Saccharomyces fragilis
-
low activity
-
2.6.1.7
Schwanniomyces occidentalis
-
low activity
-
2.6.1.7
Schwanniomyces polymorphus
-
yeast
-
2.6.1.7
Torulaspora globosa
-
low activity
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.7
-
Hansenula schneggii
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.7
16.9
-
-
Hansenula schneggii
Storage Stability
EC Number
Storage Stability
Organism
2.6.1.7
-20°C, at protein concentrations above 0.6 mg/ml, 0.02 mM pyridoxal phosphate, 0.01% mercaptoethanol, several weeks, no loss of activity
Hansenula schneggii
2.6.1.7
4°C, crystalline enzyme as suspension in 70% saturated ammonium sulfate, pH 7.2, more than 6 months
Hansenula schneggii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-2-aminohexane-1,6-dioate + 2-oxoglutarate
at about 60% the rate of L-kynurenine
636614
Hansenula schneggii
2-oxohexane-1,6-dioate + L-glutamate
-
636614
Hansenula schneggii
?
2.6.1.7
L-3-hydroxykynurenine + 2-oxoglutarate
at about 45% the rate of L-kynurenine
636614
Hansenula schneggii
xanthurenic acid + L-glutamate
-
636614
Hansenula schneggii
?
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636614
Saccharomyces fragilis
kynurenic acid + L-glutamate + H2O
-
636614
Saccharomyces fragilis
-
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636614
Cutaneotrichosporon cutaneum
kynurenic acid + L-glutamate + H2O
-
636614
Cutaneotrichosporon cutaneum
-
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636614
Schwanniomyces occidentalis
kynurenic acid + L-glutamate + H2O
-
636614
Schwanniomyces occidentalis
-
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636614
Diutina rugosa
kynurenic acid + L-glutamate + H2O
-
636614
Diutina rugosa
-
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636614
Naganishia albida
kynurenic acid + L-glutamate + H2O
-
636614
Naganishia albida
-
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636614
Torulaspora globosa
kynurenic acid + L-glutamate + H2O
-
636614
Torulaspora globosa
-
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636614
Saccharomyces cerevisiae
kynurenic acid + L-glutamate + H2O
via o-aminobenzoylpyruvic acid which is immediately converted to the intramolecularly dehydrated and cyclized form kynurenic acid
636614
Saccharomyces cerevisiae
?
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636614
Kluyveromyces marxianus
kynurenic acid + L-glutamate + H2O
via o-aminobenzoylpyruvic acid which is immediately converted to the intramolecularly dehydrated and cyclized form kynurenic acid
636614
Kluyveromyces marxianus
?
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636614
Debaryomyces hansenii
kynurenic acid + L-glutamate + H2O
via o-aminobenzoylpyruvic acid which is immediately converted to the intramolecularly dehydrated and cyclized form kynurenic acid
636614
Debaryomyces hansenii
?
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636614
Schwanniomyces polymorphus
kynurenic acid + L-glutamate + H2O
via o-aminobenzoylpyruvic acid which is immediately converted to the intramolecularly dehydrated and cyclized form kynurenic acid
636614
Schwanniomyces polymorphus
?
2.6.1.7
L-kynurenine + 2-oxoglutarate
best substrates
636614
Hansenula schneggii
kynurenic acid + L-glutamate + H2O
via o-aminobenzoylpyruvic acid which is immediately converted to the intramolecularly dehydrated and cyclized form kynurenic acid
636614
Hansenula schneggii
-
2.6.1.7
L-kynurenine + 2-oxohexanedioate
-
636614
Hansenula schneggii
kynurenic acid + 2-aminohexanedioate + H2O
-
636614
Hansenula schneggii
?
2.6.1.7
L-kynurenine + 2-oxoisohexanoate
-
636614
Hansenula schneggii
kynurenic acid + 2-aminoisohexanoate + H2O
-
636614
Hansenula schneggii
?
2.6.1.7
L-kynurenine + 3-indole-2-oxopropanoate
-
636614
Hansenula schneggii
kynurenic acid + L-tryptophan + H2O
-
636614
Hansenula schneggii
?
2.6.1.7
L-kynurenine + 3-phenyl-2-oxopropanoate
-
636614
Hansenula schneggii
kynurenic acid + L-phenylalanine + H2O
-
636614
Hansenula schneggii
?
2.6.1.7
L-kynurenine + 4-methylsulfanyl-2-oxobutyrate
-
636614
Hansenula schneggii
kynurenic acid + L-methionine + H2O
-
636614
Hansenula schneggii
?
2.6.1.7
L-leucine + 2-oxoglutarate
at about 85% the rate of L-kynurenine
636614
Hansenula schneggii
4-methyl-2-oxopentanoate + L-glutamate
-
636614
Hansenula schneggii
?
2.6.1.7
L-methionine + 2-oxoglutarate
at about 36% the rate of L-kynurenine
636614
Hansenula schneggii
4-methylsulfanyl-2-oxobutanoate + glutamate
-
636614
Hansenula schneggii
?
2.6.1.7
L-tryptophan + 2-oxoglutarate
at about 60% the rate of L-kynurenine
636614
Hansenula schneggii
3-indole-2-oxopropanoate + L-glutamate
-
636614
Hansenula schneggii
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.7
37
-
assay at
Hansenula schneggii
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.6.1.7
50
-
and below, 5 min stable between pH 6.0 and pH 10.0
Hansenula schneggii
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.7
pyridoxal 5'-phosphate
-
Hansenula schneggii
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.7
pyridoxal 5'-phosphate
-
Hansenula schneggii
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.6.1.7
-
Hansenula schneggii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.7
4-chloromercuribenzoate
-
Hansenula schneggii
2.6.1.7
Dicarboxylic acids
-
Hansenula schneggii
2.6.1.7
Hg2+
-
Hansenula schneggii
2.6.1.7
iodoacetate
-
Hansenula schneggii
2.6.1.7
additional information
not inhibited by EDTA, 2,2'-dipyridyl
Hansenula schneggii
2.6.1.7
N-ethylmaleimide
-
Hansenula schneggii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.7
1
-
2-oxoisocaproate
-
Hansenula schneggii
2.6.1.7
1.1
-
2-oxoglutarate
-
Hansenula schneggii
2.6.1.7
1.2
-
beta-phenylpyruvate
-
Hansenula schneggii
2.6.1.7
2.6
-
L-tryptophan
-
Hansenula schneggii
2.6.1.7
5
-
L-leucine
-
Hansenula schneggii
2.6.1.7
5
-
L-methionine
-
Hansenula schneggii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.7
98000
-
sedimentation equilibrium
Hansenula schneggii
2.6.1.7
102000
-
native PAGE
Hansenula schneggii
2.6.1.7
106000
-
gel filtration
Hansenula schneggii
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.7
-
Hansenula schneggii
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.7
16.9
-
-
Hansenula schneggii
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.6.1.7
-20°C, at protein concentrations above 0.6 mg/ml, 0.02 mM pyridoxal phosphate, 0.01% mercaptoethanol, several weeks, no loss of activity
Hansenula schneggii
2.6.1.7
4°C, crystalline enzyme as suspension in 70% saturated ammonium sulfate, pH 7.2, more than 6 months
Hansenula schneggii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-2-aminohexane-1,6-dioate + 2-oxoglutarate
at about 60% the rate of L-kynurenine
636614
Hansenula schneggii
2-oxohexane-1,6-dioate + L-glutamate
-
636614
Hansenula schneggii
?
2.6.1.7
L-3-hydroxykynurenine + 2-oxoglutarate
at about 45% the rate of L-kynurenine
636614
Hansenula schneggii
xanthurenic acid + L-glutamate
-
636614
Hansenula schneggii
?
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636614
Saccharomyces fragilis
kynurenic acid + L-glutamate + H2O
-
636614
Saccharomyces fragilis
-
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636614
Cutaneotrichosporon cutaneum
kynurenic acid + L-glutamate + H2O
-
636614
Cutaneotrichosporon cutaneum
-
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636614
Schwanniomyces occidentalis
kynurenic acid + L-glutamate + H2O
-
636614
Schwanniomyces occidentalis
-
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636614
Diutina rugosa
kynurenic acid + L-glutamate + H2O
-
636614
Diutina rugosa
-
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636614
Naganishia albida
kynurenic acid + L-glutamate + H2O
-
636614
Naganishia albida
-
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636614
Torulaspora globosa
kynurenic acid + L-glutamate + H2O
-
636614
Torulaspora globosa
-
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636614
Saccharomyces cerevisiae
kynurenic acid + L-glutamate + H2O
via o-aminobenzoylpyruvic acid which is immediately converted to the intramolecularly dehydrated and cyclized form kynurenic acid
636614
Saccharomyces cerevisiae
?
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636614
Kluyveromyces marxianus
kynurenic acid + L-glutamate + H2O
via o-aminobenzoylpyruvic acid which is immediately converted to the intramolecularly dehydrated and cyclized form kynurenic acid
636614
Kluyveromyces marxianus
?
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636614
Debaryomyces hansenii
kynurenic acid + L-glutamate + H2O
via o-aminobenzoylpyruvic acid which is immediately converted to the intramolecularly dehydrated and cyclized form kynurenic acid
636614
Debaryomyces hansenii
?
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636614
Schwanniomyces polymorphus
kynurenic acid + L-glutamate + H2O
via o-aminobenzoylpyruvic acid which is immediately converted to the intramolecularly dehydrated and cyclized form kynurenic acid
636614
Schwanniomyces polymorphus
?
2.6.1.7
L-kynurenine + 2-oxoglutarate
best substrates
636614
Hansenula schneggii
kynurenic acid + L-glutamate + H2O
via o-aminobenzoylpyruvic acid which is immediately converted to the intramolecularly dehydrated and cyclized form kynurenic acid
636614
Hansenula schneggii
-
2.6.1.7
L-kynurenine + 2-oxohexanedioate
-
636614
Hansenula schneggii
kynurenic acid + 2-aminohexanedioate + H2O
-
636614
Hansenula schneggii
?
2.6.1.7
L-kynurenine + 2-oxoisohexanoate
-
636614
Hansenula schneggii
kynurenic acid + 2-aminoisohexanoate + H2O
-
636614
Hansenula schneggii
?
2.6.1.7
L-kynurenine + 3-indole-2-oxopropanoate
-
636614
Hansenula schneggii
kynurenic acid + L-tryptophan + H2O
-
636614
Hansenula schneggii
?
2.6.1.7
L-kynurenine + 3-phenyl-2-oxopropanoate
-
636614
Hansenula schneggii
kynurenic acid + L-phenylalanine + H2O
-
636614
Hansenula schneggii
?
2.6.1.7
L-kynurenine + 4-methylsulfanyl-2-oxobutyrate
-
636614
Hansenula schneggii
kynurenic acid + L-methionine + H2O
-
636614
Hansenula schneggii
?
2.6.1.7
L-leucine + 2-oxoglutarate
at about 85% the rate of L-kynurenine
636614
Hansenula schneggii
4-methyl-2-oxopentanoate + L-glutamate
-
636614
Hansenula schneggii
?
2.6.1.7
L-methionine + 2-oxoglutarate
at about 36% the rate of L-kynurenine
636614
Hansenula schneggii
4-methylsulfanyl-2-oxobutanoate + glutamate
-
636614
Hansenula schneggii
?
2.6.1.7
L-tryptophan + 2-oxoglutarate
at about 60% the rate of L-kynurenine
636614
Hansenula schneggii
3-indole-2-oxopropanoate + L-glutamate
-
636614
Hansenula schneggii
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.7
37
-
assay at
Hansenula schneggii
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.6.1.7
50
-
and below, 5 min stable between pH 6.0 and pH 10.0
Hansenula schneggii