Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Asada, Y.; Sawa, Y.; Tanizawa, K.; Soda, K.
    Purification and characterization of yeast L-kynurenine aminotransferase with broad substrate specificity (1986), J. Biochem., 99, 1101-1110.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.6.1.7 pyridoxamine 5'-phosphate activation Hansenula schneggii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.7
-
Hansenula schneggii

General Stability

EC Number General Stability Organism
2.6.1.7 repeated freeze-thawing inactivates enzyme irriversible in dilute solution, below 0.1 mg/ml Hansenula schneggii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.7 3-Methyl-2-benzothiazolone hydrazone hydrochloride 0.1 mM, complete inhibition, reversible by pyridoxal phosphate Hansenula schneggii
2.6.1.7 4-chloromercuribenzoate 1 mM, 78% inhibition Hansenula schneggii
2.6.1.7 adipate 10 mM, 39% inhibition Hansenula schneggii
2.6.1.7 Dicarboxylic acids
-
Hansenula schneggii
2.6.1.7 Hg2+ 0.1 mM, 89% inhibition Hansenula schneggii
2.6.1.7 hydroxylamine 0.1 mM, complete inhibition after 30 min Hansenula schneggii
2.6.1.7 iodoacetate 1 mM, 64% inhibition Hansenula schneggii
2.6.1.7 L-Cycloserine 0.1 mM, 88% inhibition, reversible by pyridoxal phosphate Hansenula schneggii
2.6.1.7 L-Penicillamine 0.1 mM, 62% inhibition, reversible by pyridoxal phosphate Hansenula schneggii
2.6.1.7 additional information not inhibited by EDTA, 2,2'-dipyridyl Hansenula schneggii
2.6.1.7 N-ethylmaleimide
-
Hansenula schneggii
2.6.1.7 phenylhydrazine 0.1 mM, 81% inhibition, reversible by pyridoxal phosphate Hansenula schneggii
2.6.1.7 pimelate 10 mM, 53% inhibition Hansenula schneggii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.7 0.0013
-
pyridoxal 5'-phosphate pH 8.0, 37°C Hansenula schneggii
2.6.1.7 0.0032
-
pyridoxamine 5'-phosphate pH 8.0, 37°C Hansenula schneggii
2.6.1.7 1
-
2-oxoisocaproate pH 8.0, 37°C, cosubstrate L-kynurenine Hansenula schneggii
2.6.1.7 1.1
-
2-oxoglutarate pH 8.0, 37°C, cosubstrate L-kynurenine Hansenula schneggii
2.6.1.7 1.2
-
beta-phenylpyruvate pH 8.0, 37°C, cosubstrate L-kynurenine Hansenula schneggii
2.6.1.7 1.9
-
3-(2-aminobenzoyl)-L-alanine pH 8.0, 37°C Hansenula schneggii
2.6.1.7 2.6
-
L-tryptophan pH 8.0, 37°C, cosubstrate 2-oxoglutarate Hansenula schneggii
2.6.1.7 5
-
L-methionine pH 8.0, 37°C, cosubstrate 2-oxoglutarate Hansenula schneggii
2.6.1.7 5
-
L-leucine pH 8.0, 37°C, cosubstrate 2-oxoglutarate Hansenula schneggii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.7 additional information
-
amino acid composition Hansenula schneggii
2.6.1.7 52000
-
2 * 52000, SDS-PAGE Hansenula schneggii
2.6.1.7 98000
-
sedimentation equilibrium Hansenula schneggii
2.6.1.7 106000
-
gel filtration Hansenula schneggii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.7 L-kynurenine + 2-oxoglutarate Hansenula schneggii initial reaction in quinaldine pathway of kynurenine catabolism kynurenic acid + L-glutamate + H2O
-
?
2.6.1.7 L-kynurenine + 2-oxoglutarate Hansenula schneggii inducible enzyme of tryptophan catabolism kynurenic acid + L-glutamate + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.7 Hansenula schneggii
-
yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.7 polyethyleneimine, DEAE-cellulose, Bio-Gel P-300, hydroxyapatite, crystallization Hansenula schneggii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.7 16.9
-
-
Hansenula schneggii

Storage Stability

EC Number Storage Stability Organism
2.6.1.7 -20°C, at protein concentrations above 0.6 mg/ml, 0.02 mM pyridoxal phosphate, 0.01% mercaptoethanol, several weeks, no loss of activity Hansenula schneggii
2.6.1.7 4°C, crystalline enzyme as suspension in 70% saturated ammonium sulfate, pH 7.2, more than 6 months Hansenula schneggii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.7 L-kynurenine + 2-oxobutyrate less effective than 2-oxoglutarate Hansenula schneggii kynurenic acid + 2-aminobutanoate + H2O
-
?
2.6.1.7 L-kynurenine + 2-oxoglutarate broad specificity Hansenula schneggii kynurenic acid + L-glutamate + H2O
-
ir
2.6.1.7 L-kynurenine + 2-oxoglutarate best substrates Hansenula schneggii kynurenic acid + L-glutamate + H2O
-
ir
2.6.1.7 L-kynurenine + 2-oxoglutarate initial reaction in quinaldine pathway of kynurenine catabolism Hansenula schneggii kynurenic acid + L-glutamate + H2O
-
?
2.6.1.7 L-kynurenine + 2-oxoglutarate inducible enzyme of tryptophan catabolism Hansenula schneggii kynurenic acid + L-glutamate + H2O
-
?
2.6.1.7 L-kynurenine + 2-oxohexanoate
-
Hansenula schneggii kynurenic acid + L-norleucine + H2O
-
?
2.6.1.7 L-kynurenine + 2-oxoisohexanoate
-
Hansenula schneggii kynurenic acid + 2-aminoisohexanoate + H2O
-
?
2.6.1.7 L-kynurenine + 2-oxopentanoate less effective than 2-oxoglutarate Hansenula schneggii kynurenic acid + L-norvaline + H2O
-
?
2.6.1.7 L-kynurenine + 3-phenyl-2-oxopropanoate
-
Hansenula schneggii kynurenic acid + L-phenylalanine + H2O
-
?
2.6.1.7 L-kynurenine + 4-methylsulfanyl-2-oxobutyrate
-
Hansenula schneggii kynurenic acid + L-methionine + H2O
-
?
2.6.1.7 L-leucine + 2-oxoglutarate
-
Hansenula schneggii 4-methyl-2-oxopentanoate + L-glutamate
-
?

Subunits

EC Number Subunits Comment Organism
2.6.1.7 dimer 2 * 52000, SDS-PAGE Hansenula schneggii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.7 60
-
-
Hansenula schneggii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.7 20 60 linear increase of activity Hansenula schneggii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.7 50
-
and below, 5 min stable between pH 6.0 and pH 10.0 Hansenula schneggii
2.6.1.7 65
-
above, rapid inactivation Hansenula schneggii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.7 additional information
-
pI: 4.95 Hansenula schneggii
2.6.1.7 8
-
-
Hansenula schneggii

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.7 pyridoxal 5'-phosphate Km-value: 0.0013 mM Hansenula schneggii
2.6.1.7 pyridoxal 5'-phosphate required for activity, 2 mol per mol holoenzyme Hansenula schneggii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.7 9.5
-
pimelate
-
Hansenula schneggii
2.6.1.7 13
-
adipate
-
Hansenula schneggii