BRENDA - Enzyme Database

Purification and characterization of yeast L-kynurenine aminotransferase with broad substrate specificity

Asada, Y.; Sawa, Y.; Tanizawa, K.; Soda, K.; J. Biochem. 99, 1101-1110 (1986)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.6.1.7
pyridoxamine 5'-phosphate
activation
Hansenula schneggii
Crystallization (Commentary)
EC Number
Crystallization
Organism
2.6.1.7
-
Hansenula schneggii
General Stability
EC Number
General Stability
Organism
2.6.1.7
repeated freeze-thawing inactivates enzyme irriversible in dilute solution, below 0.1 mg/ml
Hansenula schneggii
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.7
3-Methyl-2-benzothiazolone hydrazone hydrochloride
0.1 mM, complete inhibition, reversible by pyridoxal phosphate
Hansenula schneggii
2.6.1.7
4-chloromercuribenzoate
1 mM, 78% inhibition
Hansenula schneggii
2.6.1.7
adipate
10 mM, 39% inhibition
Hansenula schneggii
2.6.1.7
Dicarboxylic acids
-
Hansenula schneggii
2.6.1.7
Hg2+
0.1 mM, 89% inhibition
Hansenula schneggii
2.6.1.7
hydroxylamine
0.1 mM, complete inhibition after 30 min
Hansenula schneggii
2.6.1.7
iodoacetate
1 mM, 64% inhibition
Hansenula schneggii
2.6.1.7
L-Cycloserine
0.1 mM, 88% inhibition, reversible by pyridoxal phosphate
Hansenula schneggii
2.6.1.7
L-Penicillamine
0.1 mM, 62% inhibition, reversible by pyridoxal phosphate
Hansenula schneggii
2.6.1.7
additional information
not inhibited by EDTA, 2,2'-dipyridyl
Hansenula schneggii
2.6.1.7
N-ethylmaleimide
-
Hansenula schneggii
2.6.1.7
phenylhydrazine
0.1 mM, 81% inhibition, reversible by pyridoxal phosphate
Hansenula schneggii
2.6.1.7
pimelate
10 mM, 53% inhibition
Hansenula schneggii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.7
0.0013
-
pyridoxal 5'-phosphate
pH 8.0, 37°C
Hansenula schneggii
2.6.1.7
0.0032
-
pyridoxamine 5'-phosphate
pH 8.0, 37°C
Hansenula schneggii
2.6.1.7
1
-
2-oxoisocaproate
pH 8.0, 37°C, cosubstrate L-kynurenine
Hansenula schneggii
2.6.1.7
1.1
-
2-oxoglutarate
pH 8.0, 37°C, cosubstrate L-kynurenine
Hansenula schneggii
2.6.1.7
1.2
-
beta-phenylpyruvate
pH 8.0, 37°C, cosubstrate L-kynurenine
Hansenula schneggii
2.6.1.7
1.9
-
3-(2-aminobenzoyl)-L-alanine
pH 8.0, 37°C
Hansenula schneggii
2.6.1.7
2.6
-
L-tryptophan
pH 8.0, 37°C, cosubstrate 2-oxoglutarate
Hansenula schneggii
2.6.1.7
5
-
L-leucine
pH 8.0, 37°C, cosubstrate 2-oxoglutarate
Hansenula schneggii
2.6.1.7
5
-
L-methionine
pH 8.0, 37°C, cosubstrate 2-oxoglutarate
Hansenula schneggii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.7
additional information
-
amino acid composition
Hansenula schneggii
2.6.1.7
52000
-
2 * 52000, SDS-PAGE
Hansenula schneggii
2.6.1.7
98000
-
sedimentation equilibrium
Hansenula schneggii
2.6.1.7
106000
-
gel filtration
Hansenula schneggii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.7
L-kynurenine + 2-oxoglutarate
Hansenula schneggii
initial reaction in quinaldine pathway of kynurenine catabolism
kynurenic acid + L-glutamate + H2O
-
-
-
2.6.1.7
L-kynurenine + 2-oxoglutarate
Hansenula schneggii
inducible enzyme of tryptophan catabolism
kynurenic acid + L-glutamate + H2O
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.7
Hansenula schneggii
-
yeast
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.7
polyethyleneimine, DEAE-cellulose, Bio-Gel P-300, hydroxyapatite, crystallization
Hansenula schneggii
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.7
16.9
-
-
Hansenula schneggii
Storage Stability
EC Number
Storage Stability
Organism
2.6.1.7
-20°C, at protein concentrations above 0.6 mg/ml, 0.02 mM pyridoxal phosphate, 0.01% mercaptoethanol, several weeks, no loss of activity
Hansenula schneggii
2.6.1.7
4°C, crystalline enzyme as suspension in 70% saturated ammonium sulfate, pH 7.2, more than 6 months
Hansenula schneggii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-kynurenine + 2-oxobutyrate
less effective than 2-oxoglutarate
636613
Hansenula schneggii
kynurenic acid + 2-aminobutanoate + H2O
-
636613
Hansenula schneggii
?
2.6.1.7
L-kynurenine + 2-oxoglutarate
broad specificity
636613
Hansenula schneggii
kynurenic acid + L-glutamate + H2O
-
636613
Hansenula schneggii
ir
2.6.1.7
L-kynurenine + 2-oxoglutarate
best substrates
636613
Hansenula schneggii
kynurenic acid + L-glutamate + H2O
-
636613
Hansenula schneggii
ir
2.6.1.7
L-kynurenine + 2-oxoglutarate
initial reaction in quinaldine pathway of kynurenine catabolism
636613
Hansenula schneggii
kynurenic acid + L-glutamate + H2O
-
-
-
-
2.6.1.7
L-kynurenine + 2-oxoglutarate
inducible enzyme of tryptophan catabolism
636613
Hansenula schneggii
kynurenic acid + L-glutamate + H2O
-
-
-
-
2.6.1.7
L-kynurenine + 2-oxohexanoate
-
636613
Hansenula schneggii
kynurenic acid + L-norleucine + H2O
-
636613
Hansenula schneggii
?
2.6.1.7
L-kynurenine + 2-oxoisohexanoate
-
636613
Hansenula schneggii
kynurenic acid + 2-aminoisohexanoate + H2O
-
636613
Hansenula schneggii
?
2.6.1.7
L-kynurenine + 2-oxopentanoate
less effective than 2-oxoglutarate
636613
Hansenula schneggii
kynurenic acid + L-norvaline + H2O
-
636613
Hansenula schneggii
?
2.6.1.7
L-kynurenine + 3-phenyl-2-oxopropanoate
-
636613
Hansenula schneggii
kynurenic acid + L-phenylalanine + H2O
-
-
-
-
2.6.1.7
L-kynurenine + 4-methylsulfanyl-2-oxobutyrate
-
636613
Hansenula schneggii
kynurenic acid + L-methionine + H2O
-
636613
Hansenula schneggii
?
2.6.1.7
L-leucine + 2-oxoglutarate
-
636613
Hansenula schneggii
4-methyl-2-oxopentanoate + L-glutamate
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.6.1.7
dimer
2 * 52000, SDS-PAGE
Hansenula schneggii
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.7
60
-
-
Hansenula schneggii
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.6.1.7
20
60
linear increase of activity
Hansenula schneggii
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.6.1.7
50
-
and below, 5 min stable between pH 6.0 and pH 10.0
Hansenula schneggii
2.6.1.7
65
-
above, rapid inactivation
Hansenula schneggii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.7
additional information
-
pI: 4.95
Hansenula schneggii
2.6.1.7
8
-
-
Hansenula schneggii
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.7
pyridoxal 5'-phosphate
Km-value: 0.0013 mM; required for activity, 2 mol per mol holoenzyme
Hansenula schneggii
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.6.1.7
9.5
-
pimelate
-
Hansenula schneggii
2.6.1.7
13
-
adipate
-
Hansenula schneggii
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.6.1.7
pyridoxamine 5'-phosphate
activation
Hansenula schneggii
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.7
pyridoxal 5'-phosphate
Km-value: 0.0013 mM; required for activity, 2 mol per mol holoenzyme
Hansenula schneggii
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.6.1.7
-
Hansenula schneggii
General Stability (protein specific)
EC Number
General Stability
Organism
2.6.1.7
repeated freeze-thawing inactivates enzyme irriversible in dilute solution, below 0.1 mg/ml
Hansenula schneggii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.7
3-Methyl-2-benzothiazolone hydrazone hydrochloride
0.1 mM, complete inhibition, reversible by pyridoxal phosphate
Hansenula schneggii
2.6.1.7
4-chloromercuribenzoate
1 mM, 78% inhibition
Hansenula schneggii
2.6.1.7
adipate
10 mM, 39% inhibition
Hansenula schneggii
2.6.1.7
Dicarboxylic acids
-
Hansenula schneggii
2.6.1.7
Hg2+
0.1 mM, 89% inhibition
Hansenula schneggii
2.6.1.7
hydroxylamine
0.1 mM, complete inhibition after 30 min
Hansenula schneggii
2.6.1.7
iodoacetate
1 mM, 64% inhibition
Hansenula schneggii
2.6.1.7
L-Cycloserine
0.1 mM, 88% inhibition, reversible by pyridoxal phosphate
Hansenula schneggii
2.6.1.7
L-Penicillamine
0.1 mM, 62% inhibition, reversible by pyridoxal phosphate
Hansenula schneggii
2.6.1.7
additional information
not inhibited by EDTA, 2,2'-dipyridyl
Hansenula schneggii
2.6.1.7
N-ethylmaleimide
-
Hansenula schneggii
2.6.1.7
phenylhydrazine
0.1 mM, 81% inhibition, reversible by pyridoxal phosphate
Hansenula schneggii
2.6.1.7
pimelate
10 mM, 53% inhibition
Hansenula schneggii
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.6.1.7
9.5
-
pimelate
-
Hansenula schneggii
2.6.1.7
13
-
adipate
-
Hansenula schneggii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.7
0.0013
-
pyridoxal 5'-phosphate
pH 8.0, 37°C
Hansenula schneggii
2.6.1.7
0.0032
-
pyridoxamine 5'-phosphate
pH 8.0, 37°C
Hansenula schneggii
2.6.1.7
1
-
2-oxoisocaproate
pH 8.0, 37°C, cosubstrate L-kynurenine
Hansenula schneggii
2.6.1.7
1.1
-
2-oxoglutarate
pH 8.0, 37°C, cosubstrate L-kynurenine
Hansenula schneggii
2.6.1.7
1.2
-
beta-phenylpyruvate
pH 8.0, 37°C, cosubstrate L-kynurenine
Hansenula schneggii
2.6.1.7
1.9
-
3-(2-aminobenzoyl)-L-alanine
pH 8.0, 37°C
Hansenula schneggii
2.6.1.7
2.6
-
L-tryptophan
pH 8.0, 37°C, cosubstrate 2-oxoglutarate
Hansenula schneggii
2.6.1.7
5
-
L-leucine
pH 8.0, 37°C, cosubstrate 2-oxoglutarate
Hansenula schneggii
2.6.1.7
5
-
L-methionine
pH 8.0, 37°C, cosubstrate 2-oxoglutarate
Hansenula schneggii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.7
additional information
-
amino acid composition
Hansenula schneggii
2.6.1.7
52000
-
2 * 52000, SDS-PAGE
Hansenula schneggii
2.6.1.7
98000
-
sedimentation equilibrium
Hansenula schneggii
2.6.1.7
106000
-
gel filtration
Hansenula schneggii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.7
L-kynurenine + 2-oxoglutarate
Hansenula schneggii
initial reaction in quinaldine pathway of kynurenine catabolism
kynurenic acid + L-glutamate + H2O
-
-
-
2.6.1.7
L-kynurenine + 2-oxoglutarate
Hansenula schneggii
inducible enzyme of tryptophan catabolism
kynurenic acid + L-glutamate + H2O
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.7
polyethyleneimine, DEAE-cellulose, Bio-Gel P-300, hydroxyapatite, crystallization
Hansenula schneggii
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.7
16.9
-
-
Hansenula schneggii
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.6.1.7
-20°C, at protein concentrations above 0.6 mg/ml, 0.02 mM pyridoxal phosphate, 0.01% mercaptoethanol, several weeks, no loss of activity
Hansenula schneggii
2.6.1.7
4°C, crystalline enzyme as suspension in 70% saturated ammonium sulfate, pH 7.2, more than 6 months
Hansenula schneggii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-kynurenine + 2-oxobutyrate
less effective than 2-oxoglutarate
636613
Hansenula schneggii
kynurenic acid + 2-aminobutanoate + H2O
-
636613
Hansenula schneggii
?
2.6.1.7
L-kynurenine + 2-oxoglutarate
broad specificity
636613
Hansenula schneggii
kynurenic acid + L-glutamate + H2O
-
636613
Hansenula schneggii
ir
2.6.1.7
L-kynurenine + 2-oxoglutarate
best substrates
636613
Hansenula schneggii
kynurenic acid + L-glutamate + H2O
-
636613
Hansenula schneggii
ir
2.6.1.7
L-kynurenine + 2-oxoglutarate
initial reaction in quinaldine pathway of kynurenine catabolism
636613
Hansenula schneggii
kynurenic acid + L-glutamate + H2O
-
-
-
-
2.6.1.7
L-kynurenine + 2-oxoglutarate
inducible enzyme of tryptophan catabolism
636613
Hansenula schneggii
kynurenic acid + L-glutamate + H2O
-
-
-
-
2.6.1.7
L-kynurenine + 2-oxohexanoate
-
636613
Hansenula schneggii
kynurenic acid + L-norleucine + H2O
-
636613
Hansenula schneggii
?
2.6.1.7
L-kynurenine + 2-oxoisohexanoate
-
636613
Hansenula schneggii
kynurenic acid + 2-aminoisohexanoate + H2O
-
636613
Hansenula schneggii
?
2.6.1.7
L-kynurenine + 2-oxopentanoate
less effective than 2-oxoglutarate
636613
Hansenula schneggii
kynurenic acid + L-norvaline + H2O
-
636613
Hansenula schneggii
?
2.6.1.7
L-kynurenine + 3-phenyl-2-oxopropanoate
-
636613
Hansenula schneggii
kynurenic acid + L-phenylalanine + H2O
-
-
-
-
2.6.1.7
L-kynurenine + 4-methylsulfanyl-2-oxobutyrate
-
636613
Hansenula schneggii
kynurenic acid + L-methionine + H2O
-
636613
Hansenula schneggii
?
2.6.1.7
L-leucine + 2-oxoglutarate
-
636613
Hansenula schneggii
4-methyl-2-oxopentanoate + L-glutamate
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.6.1.7
dimer
2 * 52000, SDS-PAGE
Hansenula schneggii
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.7
60
-
-
Hansenula schneggii
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.6.1.7
20
60
linear increase of activity
Hansenula schneggii
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.6.1.7
50
-
and below, 5 min stable between pH 6.0 and pH 10.0
Hansenula schneggii
2.6.1.7
65
-
above, rapid inactivation
Hansenula schneggii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.7
additional information
-
pI: 4.95
Hansenula schneggii
2.6.1.7
8
-
-
Hansenula schneggii