BRENDA - Enzyme Database

Purification and characterization of kynurenine-2-oxoglutarate aminotransferase from the liver, brain and small intestine of rats

Noguchi, T.; Minatogawa, Y.; Okuno, E.; Nakatani, M.; Morimoto, M.; Kido, R.; Biochem. J. 151, 399-406 (1975)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.7
additional information
not inhibited by aspartate, tyrosine, phenylalanine
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.7
mitochondrion
-
Rattus norvegicus
5739
-
2.6.1.7
soluble
-
Rattus norvegicus
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.7
100000
-
isoenzyme 2, sucrose density gradient centrifugation
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.7
Rattus norvegicus
-
Donryu; isoenzyme 2
-
2.6.1.7
Rattus norvegicus Donryu
-
Donryu
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.7
DEAE-cellulose, heat treatment, hydroxyapatite, CM-Sephadex C-25, Sephadex G-100
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.6.1.7
0.13
-
isoenzyme 2
Rattus norvegicus
2.6.1.7
7.8
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-kynurenine + 2-oxoglutarate
isoenzyme 2, no activity with L-tyrosine, L-phenylalanine, L-tryptophan, 5-hydroxy-L-tryptophan and L-aspartate
636610
Rattus norvegicus
kynurenic acid + L-glutamate + H2O
-
636610
Rattus norvegicus
-
2.6.1.7
L-kynurenine + 2-oxoglutarate
isoenzyme 2, no activity with L-tyrosine, L-phenylalanine, L-tryptophan, 5-hydroxy-L-tryptophan and L-aspartate
636610
Rattus norvegicus Donryu
kynurenic acid + L-glutamate + H2O
-
636610
Rattus norvegicus Donryu
-
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
2.6.1.7
37
-
assay at
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.7
6
6.5
-
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.7
pyridoxal 5'-phosphate
-
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.7
pyridoxal 5'-phosphate
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.7
additional information
not inhibited by aspartate, tyrosine, phenylalanine
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.7
mitochondrion
-
Rattus norvegicus
5739
-
2.6.1.7
soluble
-
Rattus norvegicus
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.7
100000
-
isoenzyme 2, sucrose density gradient centrifugation
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.7
DEAE-cellulose, heat treatment, hydroxyapatite, CM-Sephadex C-25, Sephadex G-100
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.6.1.7
0.13
-
isoenzyme 2
Rattus norvegicus
2.6.1.7
7.8
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-kynurenine + 2-oxoglutarate
isoenzyme 2, no activity with L-tyrosine, L-phenylalanine, L-tryptophan, 5-hydroxy-L-tryptophan and L-aspartate
636610
Rattus norvegicus
kynurenic acid + L-glutamate + H2O
-
636610
Rattus norvegicus
-
2.6.1.7
L-kynurenine + 2-oxoglutarate
isoenzyme 2, no activity with L-tyrosine, L-phenylalanine, L-tryptophan, 5-hydroxy-L-tryptophan and L-aspartate
636610
Rattus norvegicus Donryu
kynurenic acid + L-glutamate + H2O
-
636610
Rattus norvegicus Donryu
-
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
2.6.1.7
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.7
6
6.5
-
Rattus norvegicus