BRENDA - Enzyme Database

Kynurenine-oxoglutarate aminotransferase from rat kidney

Tobes, M.C.; Methods Enzymol. 142, 217-224 (1987)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.6.1.7
2-mercaptoethanol
activation
Rattus norvegicus
General Stability
EC Number
General Stability
Organism
2.6.1.7
dialysis of the apoenzyme against Tris-HCl or imidazole-HCl buffer inactivates
Rattus norvegicus
2.6.1.7
stable in phosphate buffer supplemented with pyridoxal phosphate
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.7
2-Aminoadipate
-
Rattus norvegicus
2.6.1.7
2-oxoadipate
-
Rattus norvegicus
2.6.1.7
2-oxoglutarate
substrate inhibition
Rattus norvegicus
2.6.1.7
adipate
-
Rattus norvegicus
2.6.1.7
Co2+
-
Rattus norvegicus
2.6.1.7
Dicarboxylic acids
C-2 to C-10
Rattus norvegicus
2.6.1.7
Hg2+
-
Rattus norvegicus
2.6.1.7
hydroxylamine
complete inhibition
Rattus norvegicus
2.6.1.7
KCN
complete inhibition
Rattus norvegicus
2.6.1.7
L-kynurenine
substrate inhibition above 6 mM
Rattus norvegicus
2.6.1.7
additional information
not inhibited by L-3-hydroxykynurenine at higher concentrations
Rattus norvegicus
2.6.1.7
Ni2+
-
Rattus norvegicus
2.6.1.7
phosphate
-
Rattus norvegicus
2.6.1.7
Sn2+
-
Rattus norvegicus
2.6.1.7
sulfate
-
Rattus norvegicus
2.6.1.7
Sulfate or phosphate esters of steroids
-
Rattus norvegicus
2.6.1.7
Zn2+
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.7
0.00085
-
pyridoxal 5'-phosphate
pH 6.5, 37°C
Rattus norvegicus
2.6.1.7
1
-
2-oxoglutarate
pH 6.5, 37°C
Rattus norvegicus
2.6.1.7
1.4
-
L-kynurenine
pH 6.5, 37°C
Rattus norvegicus
2.6.1.7
2
-
L-3-hydroxykynurenine
pH 6.5, 37°C
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.7
mitochondrion
-
Rattus norvegicus
5739
-
2.6.1.7
soluble
-
Rattus norvegicus
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.7
45000
-
2 * 45000, SDS-PAGE in the absence of 2-mercaptoethanol
Rattus norvegicus
2.6.1.7
45500
-
2 * 45500, SDS-PAGE in the presence of 2-mercaptoethanol
Rattus norvegicus
2.6.1.7
85000
-
gel filtration, sucrose density gradient centrifugation
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.7
L-kynurenine + 2-oxoglutarate
Rattus norvegicus
inducible enzyme of tryptophan catabolism
kynurenic acid + L-glutamate + H2O
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.7
Rattus norvegicus
-
adult albino
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.7
acid precipitation, ammonium sulfate, DEAE-cellulose, hydroxylapatite
Rattus norvegicus
Reaction
EC Number
Reaction
Commentary
Organism
2.6.1.7
L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate
transamination of the amino acid to a transient keto acid intermediate which spontaneously undergoes ring closure
Rattus norvegicus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.7
2.65
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
3,5-diiodo-L-tyrosine + 2-oxoglutarate
slightly better than L-kynurenine
636608
Rattus norvegicus
3-(2,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
636608
Rattus norvegicus
?
2.6.1.7
L-2-aminoheptane-1,7-dioate + 2-oxoglutarate
at 39.7% of the activity with 2-aminohexane-1,6-dioate
636608
Rattus norvegicus
2-oxoheptane-1,7-dioate + L-glutamate
-
636608
Rattus norvegicus
?
2.6.1.7
L-3-hydroxykynurenine + 2-oxoglutarate
no activity with D-isomer
636608
Rattus norvegicus
xanthurenic acid + L-glutamate
-
636608
Rattus norvegicus
-
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636608
Rattus norvegicus
kynurenic acid + L-glutamate + H2O
-
636608
Rattus norvegicus
-
2.6.1.7
L-kynurenine + 2-oxoglutarate
inducible enzyme of tryptophan catabolism
636608
Rattus norvegicus
kynurenic acid + L-glutamate + H2O
-
-
-
-
2.6.1.7
L-kynurenine + 2-oxohexanedioate
-
636608
Rattus norvegicus
kynurenic acid + 2-aminohexanedioate + H2O
-
636608
Rattus norvegicus
?
2.6.1.7
L-kynurenine + pyruvate
less effective than 2-oxoglutarate
636608
Rattus norvegicus
kynurenic acid + L-alanine + H2O
-
636608
Rattus norvegicus
?
2.6.1.7
L-methionine + 2-oxoglutarate
12% the rate of L-kynurenine
636608
Rattus norvegicus
4-methylsulfanyl-2-oxobutanoate + glutamate
-
636608
Rattus norvegicus
?
2.6.1.7
L-norleucine + 2-oxoglutarate
at about 30% the rate of L-kynurenine
636608
Rattus norvegicus
2-oxohexanoate + L-glutamate
-
636608
Rattus norvegicus
?
2.6.1.7
L-phenylalanine + 2-oxoglutarate
-
636608
Rattus norvegicus
phenylpyruvate + L-glutamate
-
636608
Rattus norvegicus
?
2.6.1.7
L-tryptophan + 2-oxoglutarate
16% the rate of L-kynurenine
636608
Rattus norvegicus
3-indole-2-oxopropanoate + L-glutamate
-
636608
Rattus norvegicus
?
2.6.1.7
L-tyrosine + 2-oxoglutarate
-
636608
Rattus norvegicus
3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
636608
Rattus norvegicus
?
Subunits
EC Number
Subunits
Commentary
Organism
2.6.1.7
dimer
2 * 45000, SDS-PAGE in the absence of 2-mercaptoethanol; 2 * 45500, SDS-PAGE in the presence of 2-mercaptoethanol
Rattus norvegicus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.7
37
-
assay at
Rattus norvegicus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.6.1.7
40
-
and above, 5 min, decreasing activity
Rattus norvegicus
2.6.1.7
70
-
5 min, complete inactivation
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.7
additional information
-
pI: 6.56
Rattus norvegicus
2.6.1.7
6.5
-
with L-kynurenine as substrate
Rattus norvegicus
2.6.1.7
7.5
-
with L-alpha-aminoadipate as substrate
Rattus norvegicus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.6.1.7
5
-
and below, inactivation even in the presence of pyridoxal phosphate
Rattus norvegicus
2.6.1.7
6.5
7.5
most stable
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.7
pyridoxal 5'-phosphate
Km-value: 0.00085 mM
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.6.1.7
2-mercaptoethanol
activation
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.7
pyridoxal 5'-phosphate
Km-value: 0.00085 mM
Rattus norvegicus
General Stability (protein specific)
EC Number
General Stability
Organism
2.6.1.7
dialysis of the apoenzyme against Tris-HCl or imidazole-HCl buffer inactivates
Rattus norvegicus
2.6.1.7
stable in phosphate buffer supplemented with pyridoxal phosphate
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.7
2-Aminoadipate
-
Rattus norvegicus
2.6.1.7
2-oxoadipate
-
Rattus norvegicus
2.6.1.7
2-oxoglutarate
substrate inhibition
Rattus norvegicus
2.6.1.7
adipate
-
Rattus norvegicus
2.6.1.7
Co2+
-
Rattus norvegicus
2.6.1.7
Dicarboxylic acids
C-2 to C-10
Rattus norvegicus
2.6.1.7
Hg2+
-
Rattus norvegicus
2.6.1.7
hydroxylamine
complete inhibition
Rattus norvegicus
2.6.1.7
KCN
complete inhibition
Rattus norvegicus
2.6.1.7
L-kynurenine
substrate inhibition above 6 mM
Rattus norvegicus
2.6.1.7
additional information
not inhibited by L-3-hydroxykynurenine at higher concentrations
Rattus norvegicus
2.6.1.7
Ni2+
-
Rattus norvegicus
2.6.1.7
phosphate
-
Rattus norvegicus
2.6.1.7
Sn2+
-
Rattus norvegicus
2.6.1.7
sulfate
-
Rattus norvegicus
2.6.1.7
Sulfate or phosphate esters of steroids
-
Rattus norvegicus
2.6.1.7
Zn2+
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.7
0.00085
-
pyridoxal 5'-phosphate
pH 6.5, 37°C
Rattus norvegicus
2.6.1.7
1
-
2-oxoglutarate
pH 6.5, 37°C
Rattus norvegicus
2.6.1.7
1.4
-
L-kynurenine
pH 6.5, 37°C
Rattus norvegicus
2.6.1.7
2
-
L-3-hydroxykynurenine
pH 6.5, 37°C
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.7
mitochondrion
-
Rattus norvegicus
5739
-
2.6.1.7
soluble
-
Rattus norvegicus
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.7
45000
-
2 * 45000, SDS-PAGE in the absence of 2-mercaptoethanol
Rattus norvegicus
2.6.1.7
45500
-
2 * 45500, SDS-PAGE in the presence of 2-mercaptoethanol
Rattus norvegicus
2.6.1.7
85000
-
gel filtration, sucrose density gradient centrifugation
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.7
L-kynurenine + 2-oxoglutarate
Rattus norvegicus
inducible enzyme of tryptophan catabolism
kynurenic acid + L-glutamate + H2O
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.7
acid precipitation, ammonium sulfate, DEAE-cellulose, hydroxylapatite
Rattus norvegicus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.7
2.65
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
3,5-diiodo-L-tyrosine + 2-oxoglutarate
slightly better than L-kynurenine
636608
Rattus norvegicus
3-(2,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
636608
Rattus norvegicus
?
2.6.1.7
L-2-aminoheptane-1,7-dioate + 2-oxoglutarate
at 39.7% of the activity with 2-aminohexane-1,6-dioate
636608
Rattus norvegicus
2-oxoheptane-1,7-dioate + L-glutamate
-
636608
Rattus norvegicus
?
2.6.1.7
L-3-hydroxykynurenine + 2-oxoglutarate
no activity with D-isomer
636608
Rattus norvegicus
xanthurenic acid + L-glutamate
-
636608
Rattus norvegicus
-
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636608
Rattus norvegicus
kynurenic acid + L-glutamate + H2O
-
636608
Rattus norvegicus
-
2.6.1.7
L-kynurenine + 2-oxoglutarate
inducible enzyme of tryptophan catabolism
636608
Rattus norvegicus
kynurenic acid + L-glutamate + H2O
-
-
-
-
2.6.1.7
L-kynurenine + 2-oxohexanedioate
-
636608
Rattus norvegicus
kynurenic acid + 2-aminohexanedioate + H2O
-
636608
Rattus norvegicus
?
2.6.1.7
L-kynurenine + pyruvate
less effective than 2-oxoglutarate
636608
Rattus norvegicus
kynurenic acid + L-alanine + H2O
-
636608
Rattus norvegicus
?
2.6.1.7
L-methionine + 2-oxoglutarate
12% the rate of L-kynurenine
636608
Rattus norvegicus
4-methylsulfanyl-2-oxobutanoate + glutamate
-
636608
Rattus norvegicus
?
2.6.1.7
L-norleucine + 2-oxoglutarate
at about 30% the rate of L-kynurenine
636608
Rattus norvegicus
2-oxohexanoate + L-glutamate
-
636608
Rattus norvegicus
?
2.6.1.7
L-phenylalanine + 2-oxoglutarate
-
636608
Rattus norvegicus
phenylpyruvate + L-glutamate
-
636608
Rattus norvegicus
?
2.6.1.7
L-tryptophan + 2-oxoglutarate
16% the rate of L-kynurenine
636608
Rattus norvegicus
3-indole-2-oxopropanoate + L-glutamate
-
636608
Rattus norvegicus
?
2.6.1.7
L-tyrosine + 2-oxoglutarate
-
636608
Rattus norvegicus
3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
636608
Rattus norvegicus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.6.1.7
dimer
2 * 45000, SDS-PAGE in the absence of 2-mercaptoethanol; 2 * 45500, SDS-PAGE in the presence of 2-mercaptoethanol
Rattus norvegicus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.7
37
-
assay at
Rattus norvegicus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.6.1.7
40
-
and above, 5 min, decreasing activity
Rattus norvegicus
2.6.1.7
70
-
5 min, complete inactivation
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.7
additional information
-
pI: 6.56
Rattus norvegicus
2.6.1.7
6.5
-
with L-kynurenine as substrate
Rattus norvegicus
2.6.1.7
7.5
-
with L-alpha-aminoadipate as substrate
Rattus norvegicus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.6.1.7
5
-
and below, inactivation even in the presence of pyridoxal phosphate
Rattus norvegicus
2.6.1.7
6.5
7.5
most stable
Rattus norvegicus