BRENDA - Enzyme Database

Kynurenine transaminase from Neurospora

Jakoby, W.B.; Bonner, D.M.; J. Biol. Chem. 221, 689-695 (1956)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.7
L-kynurenine
inhibition above 5 mM
Neurospora crassa
2.6.1.7
pyridoxal 5'-phosphate
at high concentrations
Neurospora crassa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.7
0.7
-
2-oxoglutarate
pH 7.5, 37°C, cosubstrate L-kynurenine
Neurospora crassa
2.6.1.7
0.7
-
pyruvate
pH 7.5, 37°C, cosubstrate L-kynurenine
Neurospora crassa
2.6.1.7
1.5
-
L-kynurenine
pH 7.5, 37°C
Neurospora crassa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.7
Neurospora crassa
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.7
1.5
-
L-kynurenine
Neurospora crassa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-3-hydroxykynurenine + 2-oxoglutarate
-
636607
Neurospora crassa
xanthurenic acid + L-glutamate
-
636607
Neurospora crassa
?
2.6.1.7
L-kynurenine + 2-oxobutyrate
less effective than 2-oxoglutarate
636607
Neurospora crassa
kynurenic acid + 2-aminobutanoate + H2O
-
636607
Neurospora crassa
?
2.6.1.7
L-kynurenine + 2-oxoglutarate
no activity with D-kynureinine
636607
Neurospora crassa
kynurenic acid + L-glutamate + H2O
-
636607
Neurospora crassa
?
2.6.1.7
L-kynurenine + 2-oxoisopentanoate
poor substrate
636607
Neurospora crassa
kynurenic acid + 2-aminoisopentanoate + H2O
-
636607
Neurospora crassa
?
2.6.1.7
L-kynurenine + 2-oxopentanoate
less effective than 2-oxoglutarate
636607
Neurospora crassa
kynurenic acid + L-norvaline + H2O
-
636607
Neurospora crassa
?
2.6.1.7
L-kynurenine + 3-indole-2-oxopropanoate
-
636607
Neurospora crassa
kynurenic acid + L-tryptophan + H2O
-
636607
Neurospora crassa
?
2.6.1.7
L-kynurenine + 3-methyl-2-oxopentanoate
poor substrate
636607
Neurospora crassa
kynurenic acid + L-isoleucine + H2O
-
636607
Neurospora crassa
?
2.6.1.7
L-kynurenine + 3-phenyl-2-oxopropanoate
-
636607
Neurospora crassa
kynurenic acid + L-phenylalanine + H2O
-
636607
Neurospora crassa
?
2.6.1.7
L-kynurenine + pyruvate
-
636607
Neurospora crassa
kynurenic acid + L-alanine + H2O
-
636607
Neurospora crassa
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.7
37
-
assay at
Neurospora crassa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.7
7.5
-
-
Neurospora crassa
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.6.1.7
6.2
9.2
approx. half-maximal activity at pH 6.2, approx. 60% of maximal activity at pH 9.2
Neurospora crassa
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.7
additional information
pyridoxamine 5'-phosphate is not a cofactor
Neurospora crassa
2.6.1.7
pyridoxal 5'-phosphate
Km-value: 0.001 mM
Neurospora crassa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.7
additional information
pyridoxamine 5'-phosphate is not a cofactor
Neurospora crassa
2.6.1.7
pyridoxal 5'-phosphate
Km-value: 0.001 mM
Neurospora crassa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.7
L-kynurenine
inhibition above 5 mM
Neurospora crassa
2.6.1.7
pyridoxal 5'-phosphate
at high concentrations
Neurospora crassa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.7
0.7
-
2-oxoglutarate
pH 7.5, 37°C, cosubstrate L-kynurenine
Neurospora crassa
2.6.1.7
0.7
-
pyruvate
pH 7.5, 37°C, cosubstrate L-kynurenine
Neurospora crassa
2.6.1.7
1.5
-
L-kynurenine
pH 7.5, 37°C
Neurospora crassa
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.7
1.5
-
L-kynurenine
Neurospora crassa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-3-hydroxykynurenine + 2-oxoglutarate
-
636607
Neurospora crassa
xanthurenic acid + L-glutamate
-
636607
Neurospora crassa
?
2.6.1.7
L-kynurenine + 2-oxobutyrate
less effective than 2-oxoglutarate
636607
Neurospora crassa
kynurenic acid + 2-aminobutanoate + H2O
-
636607
Neurospora crassa
?
2.6.1.7
L-kynurenine + 2-oxoglutarate
no activity with D-kynureinine
636607
Neurospora crassa
kynurenic acid + L-glutamate + H2O
-
636607
Neurospora crassa
?
2.6.1.7
L-kynurenine + 2-oxoisopentanoate
poor substrate
636607
Neurospora crassa
kynurenic acid + 2-aminoisopentanoate + H2O
-
636607
Neurospora crassa
?
2.6.1.7
L-kynurenine + 2-oxopentanoate
less effective than 2-oxoglutarate
636607
Neurospora crassa
kynurenic acid + L-norvaline + H2O
-
636607
Neurospora crassa
?
2.6.1.7
L-kynurenine + 3-indole-2-oxopropanoate
-
636607
Neurospora crassa
kynurenic acid + L-tryptophan + H2O
-
636607
Neurospora crassa
?
2.6.1.7
L-kynurenine + 3-methyl-2-oxopentanoate
poor substrate
636607
Neurospora crassa
kynurenic acid + L-isoleucine + H2O
-
636607
Neurospora crassa
?
2.6.1.7
L-kynurenine + 3-phenyl-2-oxopropanoate
-
636607
Neurospora crassa
kynurenic acid + L-phenylalanine + H2O
-
636607
Neurospora crassa
?
2.6.1.7
L-kynurenine + pyruvate
-
636607
Neurospora crassa
kynurenic acid + L-alanine + H2O
-
636607
Neurospora crassa
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.7
37
-
assay at
Neurospora crassa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.7
7.5
-
-
Neurospora crassa
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.6.1.7
6.2
9.2
approx. half-maximal activity at pH 6.2, approx. 60% of maximal activity at pH 9.2
Neurospora crassa