BRENDA - Enzyme Database

Kynurenine transaminase of rat kidney: a study of coenzyme dissociation

Mason, M.; J. Biol. Chem. 227, 61-68 (1957)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.6.1.7
alcohol fractionation inactivates
Rattus norvegicus
2.6.1.7
ammonium sulfate fractionation inactivates
Rattus norvegicus
2.6.1.7
dialysis against phosphate buffer inactivates, 2-oxoglutarate protects to some extent
Rattus norvegicus
2.6.1.7
freeze-thawing inactivates
Rattus norvegicus
2.6.1.7
slightly acidic buffer solutions inactivate, pyridoxal phosphate or pyridoxamine phosphate restores activity, 2-oxoglutarate protects
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.7
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.7
partial purification of kynurenine apotransaminase
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636606
Rattus norvegicus
kynurenic acid + L-glutamate + H2O
-
636606
Rattus norvegicus
-
2.6.1.7
L-kynurenine + 2-oxoisohexanoate
less effective than 2-oxoglutarate
636606
Rattus norvegicus
kynurenic acid + 2-aminoisohexanoate + H2O
-
636606
Rattus norvegicus
?
2.6.1.7
L-kynurenine + oxaloacetate
-
636606
Rattus norvegicus
kynurenic acid + L-aspartate + H2O
-
636606
Rattus norvegicus
?
2.6.1.7
L-kynurenine + pyruvate
less effective than 2-oxoglutarate
636606
Rattus norvegicus
kynurenic acid + L-alanine + H2O
-
636606
Rattus norvegicus
?
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.6.1.7
37
-
phosphate buffer, pH 6.3, inactivation within a few min, pyridoxal phosphate restores activity, only slow inactivation at pH 7.4, 2-oxoglutarate protects
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.7
pyridoxal 5'-phosphate
-
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.7
pyridoxal 5'-phosphate
-
Rattus norvegicus
General Stability (protein specific)
EC Number
General Stability
Organism
2.6.1.7
alcohol fractionation inactivates
Rattus norvegicus
2.6.1.7
ammonium sulfate fractionation inactivates
Rattus norvegicus
2.6.1.7
dialysis against phosphate buffer inactivates, 2-oxoglutarate protects to some extent
Rattus norvegicus
2.6.1.7
freeze-thawing inactivates
Rattus norvegicus
2.6.1.7
slightly acidic buffer solutions inactivate, pyridoxal phosphate or pyridoxamine phosphate restores activity, 2-oxoglutarate protects
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.7
partial purification of kynurenine apotransaminase
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.7
L-kynurenine + 2-oxoglutarate
-
636606
Rattus norvegicus
kynurenic acid + L-glutamate + H2O
-
636606
Rattus norvegicus
-
2.6.1.7
L-kynurenine + 2-oxoisohexanoate
less effective than 2-oxoglutarate
636606
Rattus norvegicus
kynurenic acid + 2-aminoisohexanoate + H2O
-
636606
Rattus norvegicus
?
2.6.1.7
L-kynurenine + oxaloacetate
-
636606
Rattus norvegicus
kynurenic acid + L-aspartate + H2O
-
636606
Rattus norvegicus
?
2.6.1.7
L-kynurenine + pyruvate
less effective than 2-oxoglutarate
636606
Rattus norvegicus
kynurenic acid + L-alanine + H2O
-
636606
Rattus norvegicus
?
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.6.1.7
37
-
phosphate buffer, pH 6.3, inactivation within a few min, pyridoxal phosphate restores activity, only slow inactivation at pH 7.4, 2-oxoglutarate protects
Rattus norvegicus