EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.59 | wild-type and mutant enzymes expressed in Escherichia coli | Saccharomyces cerevisiae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.59 | additional information | cal1-1 mutation preferentially affects Rho1p geranylgeranylation, and it can be suppressed by Rho1p overexpression, the cdc43-5 mutation in the same gene causes accumulation of Cdc42p in soluble form and can be suppressed by Cdc42p overproduction | Saccharomyces cerevisiae |
2.5.1.59 | additional information | seven different mutations in CAL1/CDC43 gene: cal1-1 and cdc43-2 to cdc43-7, all of mutants possess reduced activity and none show temperature-sensitive enzymatic activities, but all of them show temperature-sensitive growth phenotypes | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.59 | geranylgeranyl diphosphate + protein-cysteine | Saccharomyces cerevisiae | this prenylation is necessary for many proteins to interact with membrane localized at proper intracellular sites | S-geranylgeranyl-protein + diphosphate | - |
? | |
2.5.1.59 | geranylgeranyl diphosphate + protein-cysteine | Saccharomyces cerevisiae | yeast enzyme is essential for yeast cell growth | S-geranylgeranyl-protein + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.59 | Saccharomyces cerevisiae | - |
strains YOT433-2C, YOT434-2B, YOT435-1A, YOT436-3C, YOT437-2C | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.59 | recombinant wild-type and mutant enzymes | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.59 | geranylgeranyl diphosphate + protein-cysteine | prenylation, substrates are Rho, Rac, most trimeric G protein gamma subunits | Saccharomyces cerevisiae | S-geranylgeranyl-protein + diphosphate | - |
? | |
2.5.1.59 | geranylgeranyl diphosphate + protein-cysteine | this prenylation is necessary for many proteins to interact with membrane localized at proper intracellular sites | Saccharomyces cerevisiae | S-geranylgeranyl-protein + diphosphate | - |
? | |
2.5.1.59 | geranylgeranyl diphosphate + protein-cysteine | yeast enzyme is essential for yeast cell growth | Saccharomyces cerevisiae | S-geranylgeranyl-protein + diphosphate | - |
? |