EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.59 | 1-phosphono-(E,E,E)-geranylgeraniol | competitive to geranylgeranyl diphosphate, noncompetitive to GST-CDC42 | Bos taurus | |
2.5.1.59 | NPFREKKFFCAIL | biotin-gamma6, substrate inhibition at high peptide concentration | Bos taurus | |
2.5.1.59 | PD-083176 | noncompetitive to geranylgeranyl diphosphate, competitive to GST-CDC42, modest inhibitor | Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.59 | additional information | - |
additional information | comparison of Km of CAAX-substrates with X is Leu, Phe or Ser | Bos taurus | |
2.5.1.59 | 0.00027 | - |
(biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu | pH 7.0, 30°C, prenyl donor: geranylgeranyl diphosphate | Bos taurus | |
2.5.1.59 | 0.0078 | - |
(biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu | pH 7.0, 30°C, prenyl donor: farnesyl diphosphate | Bos taurus | |
2.5.1.59 | 0.016 | - |
GST-CDC42 | pH 7.0, 30°C, prenyl donor: geranylgeranyl diphosphate | Bos taurus | |
2.5.1.59 | 0.025 | - |
(biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu | pH 7.0, 30°C, prenyl donor: geranylgeranyl diphosphate | Bos taurus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.59 | Cd2+ | the zinc in enzyme can be replaced by Cd2+ | Bos taurus | |
2.5.1.59 | Cd2+ | 80% of maximal activity in the presence of 0.5 mM alone | Bos taurus | |
2.5.1.59 | Mg2+ | 20% of maximal activity in the presence of 0.5 mM alone | Bos taurus | |
2.5.1.59 | Mn2+ | 60% of maximal activity in the presence of 0.5 mM alone | Bos taurus | |
2.5.1.59 | additional information | not: Co2+ | Bos taurus | |
2.5.1.59 | additional information | metals not needed for complex formation between enzyme and geranylgeranyl diphosphate, but addition of prenyl acceptor only results in prenyl transfer in presence of Mg2+ and Zn2+ | Bos taurus | |
2.5.1.59 | Zn2+ | required, but no activity in presence of Zn2+ alone, only when either 0.1 mM Mg2+, Mn2+ or Cd2+ is additionally present | Bos taurus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.59 | geranylgeranyl diphosphate + protein-cysteine | Bos taurus | enzyme catalyses the transfer of the 20-carbon prenyl group from geranylgeranyl diphosphate to the cysteine residue near the C-termini of a variety of eukaryotic proteins | S-geranylgeranyl-protein + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.59 | Bos taurus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.59 | - |
Bos taurus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.5.1.59 | geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate | random sequential mechanism | Bos taurus | |
2.5.1.59 | geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate | enzyme and geranylgeranyl diphosphate produce a stable binary complex and the tightly bound geranylgeranyl diphosphate is transferred to a peptide acceptor, without the equilibration of enzyme-bound geranylgeranyl diphosphate with free geranylgeranyl diphosphate | Bos taurus | |
2.5.1.59 | geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate | this enzyme, along with protein farnesyltransferase, EC 2.5.1.58 and protein geranylgeranyltransferase type II, EC 2.5.1.60, constitutes the protein prenyltransferase family of enzymes | Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.5.1.59 | brain | - |
Bos taurus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.59 | farnesyl diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu | biotin-gamma6 | Bos taurus | diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFF-S-farnesyl-CAI-Leu | - |
? | |
2.5.1.59 | geranylgeranyl diphosphate + (biotin-CONH-(CH2)5-CO-)-ALEPPETEPKRKCCIF | - |
Bos taurus | diphosphate + (biotin-CONH-(CH2)5-CO-)-ALEPPETEPKRK-S-geranylgeranyl-CCIF | - |
? | |
2.5.1.59 | geranylgeranyl diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu | biotin-gamma6, preferred substrate | Bos taurus | diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFF-S-geranylgeranyl-CAI-Leu | - |
? | |
2.5.1.59 | geranylgeranyl diphosphate + GST-CDC42 | recombinant glutathione S-transferase fusion protein of CDC42Hs with a C-terminal Cys-Cys-Ile-Phe sequence | Bos taurus | diphosphate + GST-S-geranylgeranyl-CDC42 | - |
? | |
2.5.1.59 | geranylgeranyl diphosphate + protein-cysteine | enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X. X is Leu, Phe or Ser, preferred substrate: Leu | Bos taurus | S-geranylgeranyl-protein + diphosphate | - |
? | |
2.5.1.59 | geranylgeranyl diphosphate + protein-cysteine | enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X. X may be Leu, Phe, or Ser, with high concentrations of peptide substrates this specificity is lost | Bos taurus | S-geranylgeranyl-protein + diphosphate | - |
? | |
2.5.1.59 | geranylgeranyl diphosphate + protein-cysteine | enzyme catalyses the transfer of the 20-carbon prenyl group from geranylgeranyl diphosphate to the cysteine residue near the C-termini of a variety of eukaryotic proteins | Bos taurus | S-geranylgeranyl-protein + diphosphate | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.59 | additional information | - |
additional information | comparison of kcat of CAAX-substrates with X is Leu, Phe or Ser | Bos taurus | |
2.5.1.59 | 0.0057 | - |
GST-CDC42 | prenyl donor: geranylgeranyl diphosphate | Bos taurus | |
2.5.1.59 | 0.028 | - |
(biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu | prenyl donor: geranylgeranyl diphosphate | Bos taurus | |
2.5.1.59 | 0.1 | - |
(biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu | prenyl donor: farnesyl diphosphate | Bos taurus | |
2.5.1.59 | 0.12 | - |
(biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu | prenyl donor: geranylgeranyl diphosphate | Bos taurus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.59 | 0.00072 | - |
1-phosphono-(E,E,E)-geranylgeraniol | with respect to geranylgeranyl diphosphate | Bos taurus | |
2.5.1.59 | 0.00086 | - |
1-P-GGOH | with respect to GST-CDC42 | Bos taurus | |
2.5.1.59 | 0.0082 | - |
PD-083176 | with respect to GST-CDC42 | Bos taurus | |
2.5.1.59 | 0.0087 | - |
PD-083176 | with respect to geranylgeranyl diphosphate | Bos taurus |