Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Abola, A.P.; Willits, M.G.; Wang, R.C.; Long, S.R.
    Reduction of adenosine-5'-phosphosulfate instead of 3'-phosphoadenosine-5'-phosphosulfate in cysteine biosynthesis by Rhizobium meliloti and other members of the family Rhizobiaceae (1999), J. Bacteriol., 181, 5280-5287.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.10 epression of His-tagged enzyme in Escherichia coli Sinorhizobium meliloti

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.4.10 5'-AMP
-
 Sinorhizobium meliloti
1.8.4.10 additional information not inhibited by 3'-AMP Sinorhizobium meliloti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.4.10 0.003 0.004 adenosine-5'-phosphosulfate
-
 Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.10 5'-adenylyl sulfate + thioredoxin Rhizobium sp. no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin Agrobacterium tumefaciens no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin Sinorhizobium fredii no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin Rhizobium leguminosarum no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin Sinorhizobium meliloti no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin Rhizobium sp. involved in assimilatory sulfate reduction AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin Agrobacterium tumefaciens involved in assimilatory sulfate reduction AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin Sinorhizobium fredii involved in assimilatory sulfate reduction AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin Rhizobium leguminosarum involved in assimilatory sulfate reduction AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin Sinorhizobium meliloti involved in assimilatory sulfate reduction AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin Rhizobium sp. NGR234 no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin Rhizobium sp. NGR234 involved in assimilatory sulfate reduction AMP + sulfite + thioredoxin disulfide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.10 Agrobacterium tumefaciens
-
-
-
1.8.4.10 Rhizobium leguminosarum
-
-
-
1.8.4.10 Rhizobium sp.
-
-
-
1.8.4.10 Rhizobium sp. NGR234
-
-
-
1.8.4.10 Sinorhizobium fredii
-
 also known as Sinorhizobium fredii
-
1.8.4.10 Sinorhizobium meliloti P56891 also known as Sinorhizobium meliloti
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.10 recombinant His-tagged enzyme, Ni-nitrilotriacetic acid column Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.10 5'-adenylyl sulfate + thioredoxin no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS Rhizobium sp. AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS Agrobacterium tumefaciens AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS Sinorhizobium fredii AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS Rhizobium leguminosarum AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS Sinorhizobium meliloti AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin involved in assimilatory sulfate reduction Rhizobium sp. AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin involved in assimilatory sulfate reduction Agrobacterium tumefaciens AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin involved in assimilatory sulfate reduction Sinorhizobium fredii AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin involved in assimilatory sulfate reduction Rhizobium leguminosarum AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin involved in assimilatory sulfate reduction Sinorhizobium meliloti AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS Rhizobium sp. NGR234 AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.4.10 5'-adenylyl sulfate + thioredoxin involved in assimilatory sulfate reduction Rhizobium sp. NGR234 AMP + sulfite + thioredoxin disulfide
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.10 thioredoxin
-
 Rhizobium sp.
1.8.4.10 thioredoxin
-
 Agrobacterium tumefaciens
1.8.4.10 thioredoxin
-
 Sinorhizobium fredii
1.8.4.10 thioredoxin
-
 Rhizobium leguminosarum
1.8.4.10 thioredoxin activity drops approx. 5fold in the absence of thioredoxin, glutathione can not replace thioredoxin Sinorhizobium meliloti