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Literature summary extracted from

  • Nakamura, Y.; Ogihara, S.; Ohtaki, S.
    Activation by ATP of calcium-dependent NADPH-oxidase generating hydrogen peroxide in thyroid plasma membranes (1987), J. Biochem., 102, 1121-1132.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.6.3.1 2,6-dichlorophenolindophenol
-
Sus scrofa
1.6.3.1 ATP 2fold activation Sus scrofa
1.6.3.1 cytochrome c
-
Sus scrofa
1.6.3.1 menadione
-
Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.3.1 Cd2+
-
Sus scrofa
1.6.3.1 Mn2+
-
Sus scrofa
1.6.3.1 additional information inhibition by addition of hexokinase and glucose to remove ATP Sus scrofa
1.6.3.1 p-chloromercuribenzoate
-
Sus scrofa
1.6.3.1 sodium deoxycholate no activity at 5 mg/ml Sus scrofa
1.6.3.1 Triton X-100 no activity at 2 mg/ml Sus scrofa
1.6.3.1 Zn2+
-
Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.3.1 0.023
-
NADPH pH 7, 37°C, 1.1 mM Ca2+ Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.3.1 apical plasma membrane
-
Sus scrofa 16324
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.6.3.1 Ba2+ very low activity Sus scrofa
1.6.3.1 Ca2+ required for full activity Sus scrofa
1.6.3.1 Ca2+ regulates enzyme activity Sus scrofa
1.6.3.1 Ca2+ half maximal activity with 0.0001 mM Ca2+ Sus scrofa
1.6.3.1 Cd2+ inhibitory Sus scrofa
1.6.3.1 additional information Mg2+ has no effect Sus scrofa
1.6.3.1 Sr2+ can replace for Ca2+ Sus scrofa
1.6.3.1 Zn2+ inhibitory Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.3.1 NAD(P)H + H+ + O2 Sus scrofa production of hydrogen peroxide in thyroid gland that is required for thyroid hormone synthesis NAD(P)+ + H2O2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.6.3.1 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.3.1 partial Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.3.1 thyroid gland
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.3.1 0.0007
-
pH 7., 37°C, 1.1 mM Ca2+ Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.3.1 NAD(P)H + H+ + O2
-
Sus scrofa NAD(P)+ + H2O2
-
?
1.6.3.1 NAD(P)H + H+ + O2 production of hydrogen peroxide in thyroid gland that is required for thyroid hormone synthesis Sus scrofa NAD(P)+ + H2O2
-
?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.6.3.1 0.0002
-
p-chloromercuribenzoate
-
Sus scrofa