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Literature summary extracted from

  • Leutwein, C.; Heider, J.
    (R)-Benzylsuccinyl-CoA dehydrogenase of Thauera aromatica, an enzyme of the anaerobic toluene catabolic pathway (2002), Arch. Microbiol., 178, 517-524.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.8.3
-
 Thauera aromatica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.8.3 (S)-benzylsuccinyl-CoA complete inhibition Thauera aromatica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.3 0.11
-
 (R)-2-benzylsuccinyl-CoA
-
 Thauera aromatica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.8.3 45000
-
 alpha4, 4 * 45000, SDS-PAGE Thauera aromatica
1.3.8.3 200000
-
 gel filtration Thauera aromatica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein Thauera aromatica
-
 (E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.3 Aromatoleum tolulyticum
-
 grown on toluene
-
1.3.8.3 Aromatoleum tolulyticum B5
-
 grown on toluene
-
1.3.8.3 Azoarcus sp.
-
 strains T and M3, grown on toluene or m-xylene
-
1.3.8.3 Thauera aromatica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.3 Q-Sepharose, hydroxyapatite, butyl-Fractogel, Superdex HR200, recombinant enzyme Thauera aromatica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.8.3 6.4
-
 recombinant enzyme Thauera aromatica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein
-
 Thauera aromatica (E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
-
 ?
1.3.8.3 (R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein highly specific for (R)-benzylsuccinyl-CoA Thauera aromatica (E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
-
 ?
1.3.8.3 (R)-2-benzylsuccinyl-CoA + ferricenium hexafluorophosphate low activity with a mixture of phenazine methosulfate/dichlorophenol indophenol, no activity with NAD+, NADP+, dichlorophenolindophenol, ferricyanide or tetrazolium as electron acceptors, very low activity with valeryl-CoA and octanoyl-CoA Thauera aromatica (E)-2-benzylidenesuccinyl-CoA + ferrocenium hexafluorophosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.8.3 tetramer alpha4, 4 * 45000, SDS-PAGE Thauera aromatica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.8.3 50
-
-
 Thauera aromatica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.8.3 8
-
-
 Thauera aromatica

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.8.3 FAD no FMN or riboflavin detected Thauera aromatica