BRENDA - Enzyme Database

Synthesis of 3,4-cis[3H]leucocyanidin and enzymic reduction to catechin

Tanner, G.J.; Kristiansen, K.N.; Anal. Biochem. 209, 274-277 (1993)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.17.1.3
only little loss of activity after freezing and thawing
Onobrychis viciifolia
1.17.1.3
unstable in desalted extracts
Hordeum vulgare
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.1.3
0.042
-
NADPH
pH 6.8, 30°C
Onobrychis viciifolia
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.1.3
2,3-trans-3,4-cis-leucocyanidin + NADPH
Hordeum vulgare
-
(2R,3S)-catechin + NADP+ + H2O
-
-
-
1.17.1.3
2,3-trans-3,4-cis-leucocyanidin + NADPH
Onobrychis viciifolia
-
(2R,3S)-catechin + NADP+ + H2O
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.17.1.3
Hordeum vulgare
-
barley
-
1.17.1.3
Onobrychis viciifolia
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.17.1.3
caryopsis
-
Hordeum vulgare
-
1.17.1.3
leaf
-
Onobrychis viciifolia
-
Storage Stability
EC Number
Storage Stability
Organism
1.17.1.3
4°C, desalted extract, 40% loss of activity after 90 min
Hordeum vulgare
1.17.1.3
4°C, desalted extract, only little loss of activity after 90 min
Onobrychis viciifolia
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.1.3
2,3-trans-3,4-cis-leucocyanidin + NADPH
-
636458
Hordeum vulgare
(2R,3S)-catechin + NADP+ + H2O
-
-
-
-
1.17.1.3
2,3-trans-3,4-cis-leucocyanidin + NADPH
-
636458
Hordeum vulgare
(2R,3S)-catechin + NADP+ + H2O
-
636458
Hordeum vulgare
?
1.17.1.3
2,3-trans-3,4-cis-leucocyanidin + NADPH
-
636458
Onobrychis viciifolia
(2R,3S)-catechin + NADP+ + H2O
-
-
-
-
1.17.1.3
2,3-trans-3,4-cis-leucocyanidin + NADPH
-
636458
Onobrychis viciifolia
(2R,3S)-catechin + NADP+ + H2O
-
636458
Onobrychis viciifolia
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.1.3
6.6
-
-
Hordeum vulgare
1.17.1.3
6.6
-
-
Onobrychis viciifolia
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.17.1.3
6
7.5
80% activity at pH 6 and pH 7.5
Hordeum vulgare
1.17.1.3
6
7.5
80% activity at pH 6 and pH 7.5
Onobrychis viciifolia
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.17.1.3
NADH
-
Hordeum vulgare
1.17.1.3
NADH
-
Onobrychis viciifolia
1.17.1.3
NADPH
preferred over NADH
Hordeum vulgare
1.17.1.3
NADPH
preferred over NADH
Onobrychis viciifolia
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.17.1.3
NADH
-
Hordeum vulgare
1.17.1.3
NADH
-
Onobrychis viciifolia
1.17.1.3
NADPH
preferred over NADH
Hordeum vulgare
1.17.1.3
NADPH
preferred over NADH
Onobrychis viciifolia
General Stability (protein specific)
EC Number
General Stability
Organism
1.17.1.3
only little loss of activity after freezing and thawing
Onobrychis viciifolia
1.17.1.3
unstable in desalted extracts
Hordeum vulgare
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.1.3
0.042
-
NADPH
pH 6.8, 30°C
Onobrychis viciifolia
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.1.3
2,3-trans-3,4-cis-leucocyanidin + NADPH
Hordeum vulgare
-
(2R,3S)-catechin + NADP+ + H2O
-
-
-
1.17.1.3
2,3-trans-3,4-cis-leucocyanidin + NADPH
Onobrychis viciifolia
-
(2R,3S)-catechin + NADP+ + H2O
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.17.1.3
caryopsis
-
Hordeum vulgare
-
1.17.1.3
leaf
-
Onobrychis viciifolia
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.17.1.3
4°C, desalted extract, 40% loss of activity after 90 min
Hordeum vulgare
1.17.1.3
4°C, desalted extract, only little loss of activity after 90 min
Onobrychis viciifolia
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.1.3
2,3-trans-3,4-cis-leucocyanidin + NADPH
-
636458
Hordeum vulgare
(2R,3S)-catechin + NADP+ + H2O
-
-
-
-
1.17.1.3
2,3-trans-3,4-cis-leucocyanidin + NADPH
-
636458
Hordeum vulgare
(2R,3S)-catechin + NADP+ + H2O
-
636458
Hordeum vulgare
?
1.17.1.3
2,3-trans-3,4-cis-leucocyanidin + NADPH
-
636458
Onobrychis viciifolia
(2R,3S)-catechin + NADP+ + H2O
-
-
-
-
1.17.1.3
2,3-trans-3,4-cis-leucocyanidin + NADPH
-
636458
Onobrychis viciifolia
(2R,3S)-catechin + NADP+ + H2O
-
636458
Onobrychis viciifolia
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.1.3
6.6
-
-
Hordeum vulgare
1.17.1.3
6.6
-
-
Onobrychis viciifolia
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.17.1.3
6
7.5
80% activity at pH 6 and pH 7.5
Hordeum vulgare
1.17.1.3
6
7.5
80% activity at pH 6 and pH 7.5
Onobrychis viciifolia