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Literature summary extracted from
- Synthesis of 3,4-cis[3H]leucocyanidin and enzymic reduction to catechin (1993), Anal. Biochem., 209, 274-277.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.17.1.3 | only little loss of activity after freezing and thawing | Onobrychis viciifolia |
| 1.17.1.3 | unstable in desalted extracts | Hordeum vulgare |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.17.1.3 | 0.042 | - |
NADPH | pH 6.8, 30°C | Onobrychis viciifolia |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.1.3 | 2,3-trans-3,4-cis-leucocyanidin + NADPH | Hordeum vulgare | - |
(2R,3S)-catechin + NADP+ + H2O | - |
? | |
| 1.17.1.3 | 2,3-trans-3,4-cis-leucocyanidin + NADPH | Onobrychis viciifolia | - |
(2R,3S)-catechin + NADP+ + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.17.1.3 | Hordeum vulgare | - |
barley | - |
| 1.17.1.3 | Onobrychis viciifolia | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.17.1.3 | caryopsis | - |
Hordeum vulgare | - |
| 1.17.1.3 | leaf | - |
Onobrychis viciifolia | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.17.1.3 | 4°C, desalted extract, 40% loss of activity after 90 min | Hordeum vulgare |
| 1.17.1.3 | 4°C, desalted extract, only little loss of activity after 90 min | Onobrychis viciifolia |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.1.3 | 2,3-trans-3,4-cis-leucocyanidin + NADPH | - |
Hordeum vulgare | (2R,3S)-catechin + NADP+ + H2O | - |
? | |
| 1.17.1.3 | 2,3-trans-3,4-cis-leucocyanidin + NADPH | - |
Onobrychis viciifolia | (2R,3S)-catechin + NADP+ + H2O | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.3 | 6.6 | - |
- |
Hordeum vulgare |
| 1.17.1.3 | 6.6 | - |
- |
Onobrychis viciifolia |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.3 | 6 | 7.5 | 80% activity at pH 6 and pH 7.5 | Hordeum vulgare |
| 1.17.1.3 | 6 | 7.5 | 80% activity at pH 6 and pH 7.5 | Onobrychis viciifolia |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.1.3 | NADH | - |
Hordeum vulgare | |
| 1.17.1.3 | NADH | - |
Onobrychis viciifolia | |
| 1.17.1.3 | NADPH | preferred over NADH | Hordeum vulgare | |
| 1.17.1.3 | NADPH | preferred over NADH | Onobrychis viciifolia | |
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Release 2023.1 (January 2023)
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