BRENDA - Enzyme Database

Purification and characterization of a highly stable tyrosinase from Thermomicrobium roseum

Kong, K.H.; Hong, M.P.; Choi, S.S.; Kim, Y.T.; Cho, S.H.; Biotechnol. Appl. Biochem. 31, 113-118 (2000)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.18.1
2-mercaptoethanol
0.1 mM, complete inhibition
Thermomicrobium roseum
1.14.18.1
5-hydroxy-2-(hydroxymethyl)-2H-pyran-4-one
1 mM, 50% inhibition of L-dopa oxidation
Thermomicrobium roseum
1.14.18.1
Cl-
1 mM, 35% inhibition of L-dopa oxidation
Thermomicrobium roseum
1.14.18.1
diethyldithiocarbamate
1 mM, complete inhibition of L-dopa oxidation
Thermomicrobium roseum
1.14.18.1
EDTA
1 mM, 71% inhibition of L-dopa oxidation
Thermomicrobium roseum
1.14.18.1
glutathione
1 mM, 86% inhibition of L-dopa oxidation
Thermomicrobium roseum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.18.1
0.18
-
L-Dopa
-
Thermomicrobium roseum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.18.1
copper
50% activation of L-dopa oxidation
Thermomicrobium roseum
1.14.18.1
K+
50% activation of L-dopa oxidation
Thermomicrobium roseum
1.14.18.1
Mg2+
33% activation of L-dopa oxidation
Thermomicrobium roseum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.18.1
43000
-
2 * 43000, SDS-PAGE
Thermomicrobium roseum
1.14.18.1
90000
-
gel filtration
Thermomicrobium roseum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.18.1
Thermomicrobium roseum
-
ATCC 27502
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.18.1
DEAE-Sephacel, Mono Q
Thermomicrobium roseum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.18.1
2.49
-
-
Thermomicrobium roseum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.18.1
(RS)-catechin + O2
83% of activity with L-dopa
636443
Thermomicrobium roseum
?
-
-
-
?
1.14.18.1
4-hydroxybenzoic acid + O2 + AH2
50% of activity with L-dopa
636443
Thermomicrobium roseum
3,4-dihydroxybenzoic acid + H2O + A
-
636443
Thermomicrobium roseum
?
1.14.18.1
caffeic acid + 1/2 O2
17% of activity with L-dopa
636443
Thermomicrobium roseum
caffeoyl quinone + H2O
-
636443
Thermomicrobium roseum
?
1.14.18.1
catechol + 1/2 O2
125% of activity with L-dopa
636443
Thermomicrobium roseum
1,2-benzoquinone + H2O
-
636443
Thermomicrobium roseum
?
1.14.18.1
chlorogenic acid + O2
108% of activity with L-dopa
636443
Thermomicrobium roseum
chlorogenoquinone + H2O
-
636443
Thermomicrobium roseum
?
1.14.18.1
D-catechin + O2
67% of activity with L-dopa
636443
Thermomicrobium roseum
?
-
-
-
?
1.14.18.1
DL-epicatechin + O2
91% of activity with L-dopa
636443
Thermomicrobium roseum
?
-
-
-
?
1.14.18.1
dopa + 1/2 O2
-
636443
Thermomicrobium roseum
dopaquinone + H2O
-
636443
Thermomicrobium roseum
?
1.14.18.1
L-tyrosine + O2 + AH2
10% of activity with L-dopa
636443
Thermomicrobium roseum
L-dopa + H2O + A
-
636443
Thermomicrobium roseum
?
1.14.18.1
o-coumaric acid + O2
58% of activity with L-dopa
636443
Thermomicrobium roseum
?
-
-
-
?
1.14.18.1
orcin + O2
42% of activity with L-dopa
636443
Thermomicrobium roseum
?
-
-
-
?
1.14.18.1
phloroglucin + O2
87% of activity with L-dopa
636443
Thermomicrobium roseum
?
-
-
-
?
1.14.18.1
pyrogallol + O2
417% of activity with L-dopa
636443
Thermomicrobium roseum
?
-
-
-
?
1.14.18.1
resorcinol + O2
67% of activity with L-dopa
636443
Thermomicrobium roseum
?
-
-
-
?
1.14.18.1
tyramine + O2
67% of activity with L-dopa
636443
Thermomicrobium roseum
4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O
-
636443
Thermomicrobium roseum
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.18.1
dimer
2 * 43000, SDS-PAGE
Thermomicrobium roseum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.18.1
9.5
-
-
Thermomicrobium roseum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.14.18.1
7.5
10.5
less than 50% activity below pH 7.5 and above pH 10.5, L-dopa oxidation at 50°C
Thermomicrobium roseum
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.14.18.1
6
-
4°C, 20 h, about 75% loss of activity below
Thermomicrobium roseum
1.14.18.1
8.5
10
4°C, 20 h, enzyme retains more than 70% of activity in the pH-range 8.5-10.0
Thermomicrobium roseum
1.14.18.1
11
-
4°C, 20 h, about 75% loss of activity above
Thermomicrobium roseum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.18.1
2-mercaptoethanol
0.1 mM, complete inhibition
Thermomicrobium roseum
1.14.18.1
5-hydroxy-2-(hydroxymethyl)-2H-pyran-4-one
1 mM, 50% inhibition of L-dopa oxidation
Thermomicrobium roseum
1.14.18.1
Cl-
1 mM, 35% inhibition of L-dopa oxidation
Thermomicrobium roseum
1.14.18.1
diethyldithiocarbamate
1 mM, complete inhibition of L-dopa oxidation
Thermomicrobium roseum
1.14.18.1
EDTA
1 mM, 71% inhibition of L-dopa oxidation
Thermomicrobium roseum
1.14.18.1
glutathione
1 mM, 86% inhibition of L-dopa oxidation
Thermomicrobium roseum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.18.1
0.18
-
L-Dopa
-
Thermomicrobium roseum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.18.1
copper
50% activation of L-dopa oxidation
Thermomicrobium roseum
1.14.18.1
K+
50% activation of L-dopa oxidation
Thermomicrobium roseum
1.14.18.1
Mg2+
33% activation of L-dopa oxidation
Thermomicrobium roseum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.18.1
43000
-
2 * 43000, SDS-PAGE
Thermomicrobium roseum
1.14.18.1
90000
-
gel filtration
Thermomicrobium roseum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.18.1
DEAE-Sephacel, Mono Q
Thermomicrobium roseum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.18.1
2.49
-
-
Thermomicrobium roseum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.18.1
(RS)-catechin + O2
83% of activity with L-dopa
636443
Thermomicrobium roseum
?
-
-
-
?
1.14.18.1
4-hydroxybenzoic acid + O2 + AH2
50% of activity with L-dopa
636443
Thermomicrobium roseum
3,4-dihydroxybenzoic acid + H2O + A
-
636443
Thermomicrobium roseum
?
1.14.18.1
caffeic acid + 1/2 O2
17% of activity with L-dopa
636443
Thermomicrobium roseum
caffeoyl quinone + H2O
-
636443
Thermomicrobium roseum
?
1.14.18.1
catechol + 1/2 O2
125% of activity with L-dopa
636443
Thermomicrobium roseum
1,2-benzoquinone + H2O
-
636443
Thermomicrobium roseum
?
1.14.18.1
chlorogenic acid + O2
108% of activity with L-dopa
636443
Thermomicrobium roseum
chlorogenoquinone + H2O
-
636443
Thermomicrobium roseum
?
1.14.18.1
D-catechin + O2
67% of activity with L-dopa
636443
Thermomicrobium roseum
?
-
-
-
?
1.14.18.1
DL-epicatechin + O2
91% of activity with L-dopa
636443
Thermomicrobium roseum
?
-
-
-
?
1.14.18.1
dopa + 1/2 O2
-
636443
Thermomicrobium roseum
dopaquinone + H2O
-
636443
Thermomicrobium roseum
?
1.14.18.1
L-tyrosine + O2 + AH2
10% of activity with L-dopa
636443
Thermomicrobium roseum
L-dopa + H2O + A
-
636443
Thermomicrobium roseum
?
1.14.18.1
o-coumaric acid + O2
58% of activity with L-dopa
636443
Thermomicrobium roseum
?
-
-
-
?
1.14.18.1
orcin + O2
42% of activity with L-dopa
636443
Thermomicrobium roseum
?
-
-
-
?
1.14.18.1
phloroglucin + O2
87% of activity with L-dopa
636443
Thermomicrobium roseum
?
-
-
-
?
1.14.18.1
pyrogallol + O2
417% of activity with L-dopa
636443
Thermomicrobium roseum
?
-
-
-
?
1.14.18.1
resorcinol + O2
67% of activity with L-dopa
636443
Thermomicrobium roseum
?
-
-
-
?
1.14.18.1
tyramine + O2
67% of activity with L-dopa
636443
Thermomicrobium roseum
4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O
-
636443
Thermomicrobium roseum
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.18.1
dimer
2 * 43000, SDS-PAGE
Thermomicrobium roseum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.18.1
9.5
-
-
Thermomicrobium roseum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.14.18.1
7.5
10.5
less than 50% activity below pH 7.5 and above pH 10.5, L-dopa oxidation at 50°C
Thermomicrobium roseum
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.14.18.1
6
-
4°C, 20 h, about 75% loss of activity below
Thermomicrobium roseum
1.14.18.1
8.5
10
4°C, 20 h, enzyme retains more than 70% of activity in the pH-range 8.5-10.0
Thermomicrobium roseum
1.14.18.1
11
-
4°C, 20 h, about 75% loss of activity above
Thermomicrobium roseum