EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.1 | 2-mercaptoethanol | 0.1 mM, complete inhibition | Thermomicrobium roseum | |
1.14.18.1 | 5-hydroxy-2-(hydroxymethyl)-2H-pyran-4-one | 1 mM, 50% inhibition of L-dopa oxidation | Thermomicrobium roseum | |
1.14.18.1 | Cl- | 1 mM, 35% inhibition of L-dopa oxidation | Thermomicrobium roseum | |
1.14.18.1 | diethyldithiocarbamate | 1 mM, complete inhibition of L-dopa oxidation | Thermomicrobium roseum | |
1.14.18.1 | EDTA | 1 mM, 71% inhibition of L-dopa oxidation | Thermomicrobium roseum | |
1.14.18.1 | glutathione | 1 mM, 86% inhibition of L-dopa oxidation | Thermomicrobium roseum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.18.1 | 0.18 | - |
L-Dopa | - |
Thermomicrobium roseum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.1 | copper | 50% activation of L-dopa oxidation | Thermomicrobium roseum | |
1.14.18.1 | K+ | 50% activation of L-dopa oxidation | Thermomicrobium roseum | |
1.14.18.1 | Mg2+ | 33% activation of L-dopa oxidation | Thermomicrobium roseum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 43000 | - |
2 * 43000, SDS-PAGE | Thermomicrobium roseum |
1.14.18.1 | 90000 | - |
gel filtration | Thermomicrobium roseum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.18.1 | Thermomicrobium roseum | - |
ATCC 27502 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.18.1 | DEAE-Sephacel, Mono Q | Thermomicrobium roseum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 2.49 | - |
- |
Thermomicrobium roseum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.18.1 | (RS)-catechin + O2 | 83% of activity with L-dopa | Thermomicrobium roseum | ? | - |
? | |
1.14.18.1 | 4-hydroxybenzoic acid + O2 + AH2 | 50% of activity with L-dopa | Thermomicrobium roseum | 3,4-dihydroxybenzoic acid + H2O + A | - |
? | |
1.14.18.1 | caffeic acid + 1/2 O2 | 17% of activity with L-dopa | Thermomicrobium roseum | caffeoyl quinone + H2O | - |
? | |
1.14.18.1 | catechol + 1/2 O2 | 125% of activity with L-dopa | Thermomicrobium roseum | 1,2-benzoquinone + H2O | - |
? | |
1.14.18.1 | chlorogenic acid + O2 | 108% of activity with L-dopa | Thermomicrobium roseum | chlorogenoquinone + H2O | - |
? | |
1.14.18.1 | D-catechin + O2 | 67% of activity with L-dopa | Thermomicrobium roseum | ? | - |
? | |
1.14.18.1 | DL-epicatechin + O2 | 91% of activity with L-dopa | Thermomicrobium roseum | ? | - |
? | |
1.14.18.1 | dopa + 1/2 O2 | - |
Thermomicrobium roseum | dopaquinone + H2O | - |
? | |
1.14.18.1 | L-tyrosine + O2 + AH2 | 10% of activity with L-dopa | Thermomicrobium roseum | L-dopa + H2O + A | - |
? | |
1.14.18.1 | o-coumaric acid + O2 | 58% of activity with L-dopa | Thermomicrobium roseum | ? | - |
? | |
1.14.18.1 | orcin + O2 | 42% of activity with L-dopa | Thermomicrobium roseum | ? | - |
? | |
1.14.18.1 | phloroglucin + O2 | 87% of activity with L-dopa | Thermomicrobium roseum | ? | - |
? | |
1.14.18.1 | pyrogallol + O2 | 417% of activity with L-dopa | Thermomicrobium roseum | ? | - |
? | |
1.14.18.1 | resorcinol + O2 | 67% of activity with L-dopa | Thermomicrobium roseum | ? | - |
? | |
1.14.18.1 | tyramine + O2 | 67% of activity with L-dopa | Thermomicrobium roseum | 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.18.1 | dimer | 2 * 43000, SDS-PAGE | Thermomicrobium roseum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 9.5 | - |
- |
Thermomicrobium roseum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 7.5 | 10.5 | less than 50% activity below pH 7.5 and above pH 10.5, L-dopa oxidation at 50°C | Thermomicrobium roseum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 6 | - |
4°C, 20 h, about 75% loss of activity below | Thermomicrobium roseum |
1.14.18.1 | 8.5 | 10 | 4°C, 20 h, enzyme retains more than 70% of activity in the pH-range 8.5-10.0 | Thermomicrobium roseum |
1.14.18.1 | 11 | - |
4°C, 20 h, about 75% loss of activity above | Thermomicrobium roseum |