Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ito, M.; Oda, K.
    An organic solvent resistant tyrosinase from Streptomyces sp. REN-21: purification and characterization (2000), Biosci. Biotechnol. Biochem., 64, 261-267.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 5-hydroxy-2-(hydroxymethyl)-2H-pyran-4-one
-
 Streptomyces sp.
1.14.18.1 arbutin no inhibition by 1 mM arbutin Streptomyces sp.
1.14.18.1 azide no inhibition by sodium azide Streptomyces sp.
1.14.18.1 diethyldithiocarbamate
-
 Streptomyces sp.
1.14.18.1 DL-dithiothreitol
-
 Streptomyces sp.
1.14.18.1 EDTA no inhibition EDTA Streptomyces sp.
1.14.18.1 ethanol 50% ethanol, 56% inhibition Streptomyces sp.
1.14.18.1 L-cysteine
-
 Streptomyces sp.
1.14.18.1 methanol 50% inhibition in 50% methanol Streptomyces sp.
1.14.18.1 additional information not inhibited by 1 mM arbutin, or 0.2 mM Cu2+, Fe3+, Zn2+, Mg2+ and Ca2+ Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 1.25
-
 L-tyrosine
-
 Streptomyces sp.
1.14.18.1 1.64
-
 DL-tyrosine
-
 Streptomyces sp.
1.14.18.1 1.98
-
 D-tyrosine
-
 Streptomyces sp.
1.14.18.1 4.14
-
 L-Dopa
-
 Streptomyces sp.
1.14.18.1 9.56
-
 DL-dopa
-
 Streptomyces sp.
1.14.18.1 28.7
-
 D-Dopa
-
 Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 32000
-
 x * 32000, SDS-PAGE Streptomyces sp.

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.14.18.1 Acetone no loss of activity after 20 h at 30°C in 20% acetone Streptomyces sp.
1.14.18.1 dimethyl sulfoxide no loss of activity after 20 h at 30°C in 20% dimethylsulfoxide Streptomyces sp.
1.14.18.1 Ethanol 30% ethanol, 30°C, pH 7.0, 17% loss of activity after 20 h Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Mushroom
-
-
-
1.14.18.1 Streptomyces sp.
-
 REN-21, organic solvent resistant enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 ammonium sulfate, DEAE-cellulose, QAE-Sephadex A-50, Toyopearl HW-55 Streptomyces sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.1 130
-
-
 Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 D-dopa + 1/2 O2
-
 Streptomyces sp. dopaquinone + H2O
-
 r
1.14.18.1 D-dopa + O2
-
 Streptomyces sp. dopaquinone + H2O
-
 r
1.14.18.1 D-tyrosine + O2
-
 Streptomyces sp. dopaquinone + H2O
-
 r
1.14.18.1 D-tyrosine + O2
-
 Streptomyces sp. ?
-
 r
1.14.18.1 DL-dopa + 1/2 O2
-
 Streptomyces sp. dopaquinone + H2O
-
 r
1.14.18.1 DL-DOPA + O2
-
 Streptomyces sp. dopaquinone + H2O
-
 r
1.14.18.1 DL-tyrosine + O2
-
 Streptomyces sp. dopaquinone + H2O
-
 r
1.14.18.1 L-epicatechin + O2 155% of activity with L-dopa Streptomyces sp. ?
-
 ?
1.14.18.1 L-tyrosine + O2 + AH2
-
 Streptomyces sp. L-dopa + H2O + A
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.1 ?
-
 Mushroom
1.14.18.1 ? x * 32000, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.18.1 35
-
 with L-dopa Streptomyces sp.
1.14.18.1 40
-
-
 Mushroom

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.18.1 2.27
-
 D-tyrosine
-
 Streptomyces sp.
1.14.18.1 74.2
-
 DL-tyrosine
-
 Streptomyces sp.
1.14.18.1 81.4
-
 L-tyrosine
-
 Streptomyces sp.
1.14.18.1 117
-
 D-Dopa
-
 Streptomyces sp.
1.14.18.1 1120
-
 L-Dopa
-
 Streptomyces sp.
1.14.18.1 1170
-
 DL-dopa
-
 Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 7
-
 with L-dopa Streptomyces sp.